The invention provides a method for measuring urotropine content of rice and flour and a rice-flour product. The method comprises the following steps: 1, making a product to be measured into a sample;2, weighing the sample to a centrifuging tube, adding
ammonium acetate for performing ultrasonic extraction and centrifuging, and placing supernatant into a
volumetric flask; 3, continuously adding the
ammonium acetate into the centrifuging tube, performing ultrasonic extraction and centrifuging, placing the supernatant into the
volumetric flask used in step 2, and metering the volume of the supernatant to 10 mL with the
ammonium acetate to obtain a clear sample liquid; 4, performing filtering with a water-phase
microfiltration membrane to obtain a sample liquid to be measured; 5, measuring peak areas of a urotropine standard working liquid and the sample liquid to be measured with an efficient
liquid phase chromatograph to obtain a
standard curve equation; 6, calculating the urotropine content in the product to be measured. The method has the characteristics of high urotropine extraction efficiency, easiness in operation, high accuracy, high stability and high
repeatability, so thata stable and reliable method for detecting urotropine in the rice-flour product is provided.