137results about How to "Improve growth characteristics" patented technology

Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate dehydrogenase (GDH) gene is introduced into a plant together with a regulator sequence suitable for over expressing the sequence encoding GDH gene in plant cells.

Compositions and methods are provided for the optimized expansion and implantation of mesenchymal stem cells into a patient in need thereof. Autologous mesenchymal stem cells (MSCs) to a patient in need of MSCs are harvested, expanded within novel growth parameters under the influence of autologous growth factors located on the patient's platelets.

Methods and associated apparatus for automatically growing agricultural crops vertically and/or in a continuous fashion throughout each year (Vertical Farm) in combination with contiguous and co-located production of biofuel, food, biomass for the purpose of carbon sequestering (carbon credits), and biomass electric power generation. A process that incorporates vast arrays of continuous-loop conveyors, towering upon vertical framework, which allow potted plants to be transported throughout all stages of maturity in a manner which substantially multiplies yield per acre, allows production to proceed in both natural and artificial light, allows production and harvesting to be automated, and allows production to proceed in conditions which are highly favorable to plants but unfavorable to humans. The entire apparatus can be constructed of lightweight, cost-effective materials which afford mass-production and mass-array into vast automatic growing operations.

A method is provided for raising poultry, such as chickens, for food production. In a first preferred embodiment, the method includes the steps of: providing a facility for housing the poultry, providing at least one light-absorbing ventilation fan associated with the facility for ventilating the facility, exposing an interior of the facility to natural light cycles of an outside environment for a first period and regulating light cycles of the interior for a second period, thereby mimicking daylight duration variation representative of seasonal changes for stimulating sexual development of the poultry. In a second preferred embodiment, the method includes the steps of: providing a facility for housing the poultry, providing at least one light-absorbing ventilation fan associated with the facility for ventilating the facility, limiting exposure of an interior of the facility to light to produce a brown-out effect therein for enhancing physical development of the poultry.

The invention discloses a method for sequential culture of human umbilical cord blood mesenchymal stem cells by using two culture media. A lymphocyte separation medium density gradient centrifugation method is used for separating umbilical cord blood mononuclear cells, a dulbecco modified eagle medium (DMEM)/F12 is used for primary culture of the mesenchymal stem cells of the human umbilical cord blood, and the method increases adherence of the cells, reduces growth of osteoclast like cells remarkably, facilitates formation of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) colonies, and greatly improves successful rate of culture of the hUCB-MSCs. The DMEM/F12 is used continuously for subculturing to a P2 generation, the method is combined by a using enzymatic digestion and differential velocity adherent method simultaneously, and the method facilitates purification of P1-P2 generation cells remarkably. A P3 generation and the following generations are cultured by using an Oricell human umbilical cord mesenchymal stem cell culture medium, marker proteins and good morphological characteristics and growth characteristics of the hUCB-MSCs are maintained, and multilineage differentiation potential of the hUCB-MSCs is maintained. In addition, costs of the culture media adopted by the method are reduced remarkably.

The invention relates to a special rhodococcus ruber (CGMCC NO. 17012) fermentation method, a preparation process of adjuvants with different dosage forms and an application as an adjuvant in animalvaccines. Rhodococcus ruber strains are separated from a farm and obtained through single colony cloning purification and identification, the invention provides a special fermentation process where the strain inactivation product is used as an animal vaccine adjuvant, and provides a preparation process of different dosage forms of adjuvants containing the strain and the product. Animal experimentsprove that when the adjuvant product is used for univalent or multivalent animal vaccines, particularly Newcastle disease inactivated vaccine, avian influenza inactivated vaccine and swine fever livevaccine, definite non-specific immune enhancement effect can be provided, specifically, the antibody peak value level induced by the animal vaccine is obviously improved, the time for producing the protective antibody level is advanced, the antibody maintenance period is prolonged, and the immune effect of the animal vaccines is enhanced.

The invention discloses a drug substrate fertilizer particle agent to prevent cotton seeding pest, which is characterized by the following: adopting nitrogen, phosphor, potassium or composite fertilizer as carrier; allocating imidacloprid, thiram, triazole ketone and adjuvant according to certain proportion; making the drug particle agent; modifying the nourishing condition and seeding growing environment; reinforcing pest-proof and growing property.

The invention discloses a doping gadolinium yttrium scandium gallium garnet, a gadolinium yttrium scandium gallium aluminium garnet and a method of the fused mass normal crystal for preparing the gadolinium yttrium scandium gallium garnet and the gadolinium yttrium scandium gallium aluminium garnet crystal or other corresponding compound for preparing; mixing the prepared raw material fully and pressing forming, and becoming initial raw material for crystal growth after high temperature calcination or non high temperature calcination; adding the growth initial raw material into a pot for heating fully to melt and becoming an initial melt of crystal growth with melt method, the obtained crystal can be used as a solid laser working substance.

The invention relates to a Newcastle disease chimeric virus marker vaccine strain as well as a construction method and application thereof, and belongs to the field of rescue and application of Newcastle disease chimeric vaccine strains. A Newcastle disease virus reverse genetic operation platform is utilized to mutate an F protein cleavage site of a Newcastle disease gene GVII type strain into acleavage site of an attenuated strain, F and HN genes of a mutated gene VII type Newcastle disease strain and NP, P, M and L of a gene II type NDV La Sota strain construct a full-length chimeric cDNAsequence, and a 18bp nucleotide marker sequence is inserted into a non-coding region between P and M. A Newcastle disease chimeric virus NDV DC strain is obtained through transfection cell rescue. Theconstructed chimeric virus can reach relatively high culture titer in chick embryos and cells. The chimeric strain contains envelope surface glycoprotein of the gene VII type Newcastle disease strain, contains a skeleton of the gene II type strain, and has immunogenicity of the Newcastle disease gene VII type strain and high reproduction and high safety characteristics of the gene II type La Sotastrain.

A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.

The invention belongs to the technical field of plant tissue culture and discloses a dendrobenthemia japonica tissue culture method. The dendrobenthemia japonica tissue culture method comprises the steps of pretreating, performing primary culture, enrichment culture and strong seedling culture, planting in a rooting medium and transplanting, wherein in the culturing process, the external conditions are as follows: pH is 5.8-6.0, the culture temperature is 23-25 DEG C, the illumination period is 8-10h/d, and the illumination intensity is 1800-2000lux. The culture method has the benefits that 1. with the adoption of the culture method, a growth coefficient is high, a rooting rate is high, and a cultured plant has excellent growth characteristic; and 2. large scale production can be achieved by the culture method, and the reproduction efficiency is high.

A fertilizer product for delivering starter fertilizer to crops in soils as an alternative to traditional phosphorus applications to enhance rapid early plant development. Specifically, the fertilizer product comprises a synergistic mixture of:

from about 0.5 to about 1 part Mg: from about 2.4 to about 2.8 parts P₂O₅: from about 1.4 to about 2.8 parts Zn.

The invention provides a vaccine composition for infectious rhinitis of chicken. The vaccine composition comprises an immunizing dose of Avibacterium paragallinarum type A antigen and/or Avibacterium paragallinarum type C antigen and a carrier. The invention also provides a preparation method and application of the vaccine composition. The immune effect of the vaccine composition provided by the invention is superior to the immune effect of vaccines prepared from conventional strains; the content of the antigen in the vaccine composition provided by the invention can be substantially decreased, and the vaccine composition still has good immune effect; so production cost is greatly reduced.

The invention relates to the field of chemical fertilizers, in particular to a compound controlled-release fertilizer special for spring corns and a production method of the compound controlled-release fertilizer. The compound controlled-release fertilizer special for spring corns is composed of the following components in parts by weight: 300-500 parts of nitrogenous fertilizer, 100-170 parts of phosphate fertilizer, 120-235 parts of potassic fertilizer, and 3-15 parts of a slow controlled release long-acting agent. The nutrient supply, the efficiency and the time of the compound controlled-release fertilizer special for spring corns are synchronized with the crop growth requirements, the special proportion of nitrogen, phosphorus and potassium of the special fertilizer in the soil for crops can be enhanced and the formula balanced fertilizing is achieved.

The invention discloses feed with a function of improving goat meat quality and belongs to the technical field of feed. The feed is prepared from substances as follows: soybean meal, cottonseed cakes, wheat bran, corn flour, sweet potato flour, vinegar residues, salt, alfalfa, shrimp shell powder, bone meal, olive oil, mountain flour additives and nutrition additives. The prepared feed is good in palatability, rich in nutritional ingredients and reasonable in nutritional ingredient proportion and can effectively improve organism immunity and growth characteristics of goats, thereby improving nutritive value and edible quality of goat meat very well, meat yield can be increased by about 25%, and the economic benefits are higher.

The invention discloses pollution-free special pesticide for broccoli. The pollution-free special pesticide is prepared from the following substances: serissa serissoide, pericarpium zanthoxyli, golden cypress, melia azedarach leaves, menthe leaves, grape seeds, radix polygalae, radix sanguisorbae, chrysanthemum leaves, modified attapulgite and modified bentonite. Traditional Chinese medicine extracting liquid, the modified bentonite and the modified attapulgite are compounded to obtain finished-product pesticide; all the components interact, so that the pollution-free special pesticide has a very good healing effect on common downy mildew, soft rot, damping off, sclerotinia stem rot and the like of the broccoli; the healing rate reaches up to 97.5 percent or more and the diseases do not easily recur; drug resistance is not easy to generate and the stability of the pesticide is better; beneficial components are not easy to decompose and the quality guarantee period can be prolonged to be about three times; after the pollution-free special pesticide is applied, growth properties of broccoli plants are improved better; the content of flavonoids in the cultured broccolis is improved by 30 percent to 40 percent and the content of iron and zinc elements is improved by 10 percent to 20 percent, so that the broccoli has very good edible value.

The invention relates to transgenic plants exhibiting enhanced growth rates, seed and fruit yields, and overall biomass yields, as well as methods for generating growth-enhanced transgenic plants. In one embodiment, transgenic plants engineered to over-express glutamine phenylpyruvate transaminase (GPT) are provided.