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Dendrobenthemia japonica tissue culture method

A Hong Kong four-photo flowering and tissue culture technology, which is applied in the field of plant tissue culture, can solve the problems of affecting seedling quality, seedling variation, and low reproductive efficiency, and achieve excellent growth characteristics, high multiplication coefficient, and high reproductive efficiency.

Inactive Publication Date: 2015-06-24
JIANGSU BIYUNTIAN AGRI & FORESTRY TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Seed propagation seedlings in the prior art will produce variation, cannot maintain good properties and seedlings are not neat, affecting the quality of seedlings;
[0009] Although cutting and tillering propagation in the prior art belongs to asexual propagation and can maintain excellent traits, the reproductive efficiency is low and the speed is slow, which is not conducive to the mass propagation and promotion of excellent varieties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The primary medium includes: mother medium + sucrose 20000mg / L + carrageenan 6500mg / L + 6-BA 3.2mg / L + NAA 0.15mg / L;

[0040] The subculture medium includes: mother culture medium + sucrose 20000mg / L + carrageenan 6500mg / L + 6-BA 2.5mg / L + NAA 0.061mg / L,

[0041] The strong seedling medium includes: mother medium + sucrose 20000mg / L + carrageenan 6500mg / L + 6-BA 1.5mg / L + NAA 0.2mg / L,

[0042] The rooting medium includes: mother medium + sucrose 20000mg / L + carrageenan 6500mg / L + IBA 0.5mg / L + NAA 0.2mg / L.

[0043] The macroelement composition of the primary culture medium is: potassium nitrate 3600mg / L, ammonium nitrate 3000mg / L, magnesium sulfate heptahydrate 320mg / L, potassium dihydrogen sulfate 160mg / L, calcium chloride dihydrate 440mg / L.

[0044] The composition of the macroelements in the subculture proliferation medium and strong seedling medium is: potassium nitrate 1800mg / L, ammonium nitrate 1500mg / L, magnesium sulfate heptahydrate 320mg / L, potassium dihydroge...

Embodiment 2

[0050] The primary medium includes: mother medium + sucrose 25000mg / L + carrageenan 6000mg / L + 6-BA 3mg / L + NAA 0.1mg / L;

[0051] The subculture medium includes: mother culture medium + sucrose 25000mg / L + carrageenan 6000mg / L + 6-BA 2mg / L + NAA 0.06mg / L,

[0052] The strong seedling medium includes: mother medium + sucrose 25000mg / L + carrageenan 6000mg / L + 6-BA 1mg / L + NAA 0.1mg / L,

[0053] The rooting medium includes: mother medium + sucrose 25000mg / L + carrageenan 6000mg / L + IBA 0.2mg / L + NAA 0.1mg / L.

[0054] The major element composition in the primary culture medium is: potassium nitrate 1600mg / L, ammonium nitrate 1400mg / L, magnesium sulfate heptahydrate 300mg / L, potassium dihydrogen sulfate 150mg / L, calcium chloride dihydrate 200mg / L;

[0055] The composition of macroelements in subculture medium and strong seedling medium is: Potassium nitrate 2000mg / L, Ammonium nitrate 1400mg / L, Magnesium sulfate heptahydrate 340mg / L, Potassium dihydrogen sulfate 180mg / L, Calcium ch...

Embodiment 3

[0061] The primary medium includes: mother medium + sucrose 35000mg / L + carrageenan 7000mg / L + 6-BA 4mg / L + NAA 0.2mg / L;

[0062] The subculture medium includes: mother culture medium + sucrose 35000mg / L + carrageenan 7000mg / L + 6-BA 3mg / L + NAA 0.12mg / L,

[0063] The strong seedling medium includes: mother medium + sucrose 35000mg / L + carrageenan 7000mg / L + 6-BA 2mg / L + NAA 0.3mg / L,

[0064] The rooting medium includes: mother medium + sucrose 35000mg / L + carrageenan 7000mg / L + IBA 1mg / L + NAA 0.3mg / L.

[0065] The major element composition in the primary culture medium is: potassium nitrate 4000mg / L, ammonium nitrate 3200mg / L, magnesium sulfate heptahydrate 340mg / L, potassium dihydrogen sulfate 180mg / L, calcium chloride dihydrate 480mg / L;

[0066] The composition of macroelements in the subculture proliferation medium and strong seedling medium is: potassium nitrate 3500mg / L, ammonium nitrate 3000mg / L, magnesium sulfate heptahydrate 340mg / L, potassium dihydrogen sulfate 180...

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Abstract

The invention belongs to the technical field of plant tissue culture and discloses a dendrobenthemia japonica tissue culture method. The dendrobenthemia japonica tissue culture method comprises the steps of pretreating, performing primary culture, enrichment culture and strong seedling culture, planting in a rooting medium and transplanting, wherein in the culturing process, the external conditions are as follows: pH is 5.8-6.0, the culture temperature is 23-25 DEG C, the illumination period is 8-10h / d, and the illumination intensity is 1800-2000lux. The culture method has the benefits that 1. with the adoption of the culture method, a growth coefficient is high, a rooting rate is high, and a cultured plant has excellent growth characteristic; and 2. large scale production can be achieved by the culture method, and the reproduction efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a Hong Kong Sizhaohua tissue culture method. Background technique [0002] Dendrobenthemia japonica (Dendrobenthemia japonica), also known as litchi and vatica, is an evergreen tree of the genus Dendrobenthemia in the family Cornaceae. Young branches and young leaves sparsely covered with brown fine hairs. Because there are 2 pairs of large yellow-white petal-shaped bracts outside the inflorescence; the fruit is ripe red or purple-red like strawberry and lychee, so it is named mountain lychee. Polyflower fruit spherical red. The drupe is aggregated into a spherical aggregate fruit pulp, matures from September to October, and turns purple after ripening. The fruit tastes sweet and can be eaten and used for wine making. [0003] The Hong Kong Sizhaohua tree is round and umbrella-shaped, with bright leaves and graceful tree posture. The bracts of the inf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李波郑国强姜继新余旭峰徐惠芳宋苗
Owner JIANGSU BIYUNTIAN AGRI & FORESTRY TECH
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