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Plants having improved growth characteristics and method for making the same

A technology for growth characteristics, plants, applied in the field of molecular biology, which can solve problems such as loss-of-function phenotypes

Inactive Publication Date: 2008-05-28
CROPDESIGN NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It is known in the art that expression of truncated receptor-like kinases often results in a loss-of-function phenotype

Method used

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  • Plants having improved growth characteristics and method for making the same
  • Plants having improved growth characteristics and method for making the same
  • Plants having improved growth characteristics and method for making the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0155] Example 1: Identification of Nucleic Acid Sequence Related Sequences Used in the Method of the Present Invention

[0156] Using database sequence search tools such as the Basic Local Alignment Tool (BLAST) (Altschul et al. (1990) J. Mol. Biol. 215:403-410; and Altschul et al. (1997) Nucleic Acids Res. 25:3389-3402), at Identify related sequences (full-length cDNA, EST or Genome). The BLAST program is used to find local similarities between sequences by comparing nucleic acid or polypeptide sequences to sequence databases and by calculating the statistical significance of the matches. For example, the TBLASTN algorithm is applied to polypeptides encoded by nucleic acids of the present invention, using default settings, with a filter turned on to ignore sequences of low complexity. Analysis output windows are pairwise comparisons and are ordered according to probability scores (E-values), which reflect the probability that a particular alignment will occur by chance (th...

Embodiment 2

[0160] Embodiment 2: gene cloning

[0161] Arabidopsis RKS11 (internal code CDS3142, SEQ ID NO: 1 ) was amplified by PCR using an Arabidopsis seedling cDNA library (Invitrogen, Paisley, UK) as a template. After the RNA extracted from the seedlings was reverse transcribed, the cDNA was cloned into pCMV Sport6.0. The library has an average insert size of 1.5 kb and an original clone number of 1.59 × 10 7 cfu. in 6×10 11 After the first amplification of cfu / ml, the original titer was determined to be 9.6 × 10 5 cfu / ml. After plasmid extraction, 200 ng template was used in 50 μl PCR mix. The primers used for PCR amplification of the RKS11 coding sequence are Prm06771 (SEQ ID NO 3, sense) and Prm06772 (SEQ ID NO 4, reverse complement), which include the AttB site of Gateway recombination. PCR was performed using Hifi Taq DNA polymerase under standard conditions. The 2020 bp RKS11 PCR fragment (with attB site) was also amplified and purified by standard methods. Then carry o...

Embodiment 3

[0162] Example 3: Vector construction and rice transformation

[0163] Next, an LR reaction was performed using the entry clone p424 together with the designated vector p0831 for rice transformation. This vector contains the following as functional elements within the T-DNA borders: a plant selectable marker; a visible marker expression cassette; a Gateway expression cassette designed for LR in vivo recombination with the sequence of interest already cloned into the entry clone. The rice promoter (SEQ ID NO: 19) for embryo- and aleurone-specific expression was located upstream of this Gateway cassette.

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PUM

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Abstract

The present invention concerns a method for improving the growth characteristics of plants by increasing expression of at least part of a Leucine Rich Repeat Receptor-Like Kinase (RKS11, RKS4 or an orthologue of these). One such method comprises introducing into a plant a nucleic acid encoding at least part of a Leucine Rich Repeat Receptor-Like Kinase (RKS11 or RKS4 or an orthologue thereof). The invention also relates to transgenic plants having introduced therein a nucleic acid or variant thereof encoding at least part of a Leucine Rich Repeat Receptor-Like Kinase (RKS11 or RKS4 or an orthologue thereof), which plants have improved growth characteristics relative to corresponding wild type plants. The present invention also concerns constructs useful in the methods of the invention.

Description

field of invention [0001] The present invention relates generally to the field of molecular biology, and to methods of improving plant growth characteristics. More specifically, the present invention relates to improving plant growth by increasing expression in plants of at least part of a Leucine Rich Repeat Receptor-Like Kinase (RKS11 or RKS4 or their orthologs) Features especially yield methods. The present invention also relates to plants having increased expression of at least part of the leucine-rich repeat receptor-like kinase (RKS11 or RKS4 or their orthologs), which plants have improved growth characteristics relative to corresponding wild-type plants . The invention also provides constructs useful in the methods of the invention. Background of the invention [0002] Given the ever-increasing world population and the dwindling area of ​​land arable for agriculture, research into improving agricultural efficiency has been stimulated. A conventional approach to cr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/02C12N15/54
CPCC12N9/1205C12N15/8261Y02A40/146
Inventor C·勒佐
Owner CROPDESIGN NV
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