41results about How to "Guaranteed normal proliferation" patented technology

The invention relates to chimeric antigen receptors (CAR), particularly relates to dual-signal independent chimeric antigen receptors (dsCAR), and also relates to immune response cells of the dual-signal independent chimeric antigen receptors (dsCAR) and uses of the immune response cells in preparation of drugs for treatment of malignant tumor and virus infected diseases. In detail, the dual-signal independent chimeric antigen receptors (dsCAR) can respectively identify two different family antigens of tumor cells and can respectively transmit two T-cell-activation related signals. One of the CAR can transmit a first T-cell-activation related signal by combing a ligand of a tumor specific antigen or a tumor-associated antigen to decide T-cell killing specificity, and the other CAR can transmit a second T-cell-activation related signal by combing a ligand of a membrane receptor (such as EGFR (epidermal growth factor receptor) family protein) widely expressed by the tumor cells to promote T cell activation, proliferation and survival. The dual-signal independent chimeric antigen receptors (dsCAR) can avoid the potential safety problems on the basis of maintaining curative effects of second generation and third generation CAR.

The invention provides a method for detecting toxicity of antibiotic-type substances by utilizing luminous bacteria. The method comprises the following step of: prolonging the action time of antibiotic-type substances and the luminous bacteria to12-36 hours, preferably 24 hours. In the technical scheme provided by the invention, the action time of the antibiotic-type substances and the luminous bacteria is prolonged, so that the proliferation of the luminous bacteria during the period is ensured; and through the method, good timeliness of the accurate characterization of the biotoxicity of antibiotic can be realized, the detection result is more accurate on the basis of guaranteeing the timeliness, and the detection of toxicity delay of antibiotic-type pollutants is realized.

The invention discloses a multi-potent stem cell culture medium. The multi-potent stem cell culture medium comprises a basic culture medium and following added components: L-ascorbic acid-2-magnesium phosphate, sodium selenite, human insulin, ferric citrate, an alkaline basic fibroblast growth factor and a transforming growth factor beta1. The sodium selenite is successfully introduced into a multi-potent stem cell culture medium system to replace human apo-transferrin, so that the production cost of the multi-potent stem cell culture medium can be reduced; exogenous pathogenic risks are reduced; the multi-potent stem cell culture medium can guarantee normal proliferation of cells in a multi-potent stem cell culture process and the multi-potent property of the stem cells can also be maintained.

The invention discloses a long-term multiplication and storage method for embryonic callus of sweet potatoes. The method comprises the processes of selection of successive embryonic callus, successive multiplication of the embryonic callus, and induction of the embryonic callus to mature embryo regeneration. The method has the advantages that: the process flow is simple, the multiplication rate of the embryonic callus is high, and the successive time is long; the technical problem that the long-term utilization of the embryonic callus is limited because the materials are limited by seasons and the embryonic retaining time is short and the like in the sweet potato tissue culture process is solved; a good receptor material is provided for the transgene technology of the sweet potatoes; and a large amount of efficient regenerated material is provided for annually producing the tissue culture seedlings of the sweet potatoes.

The invention provides a method of manufacturing tissue engineering skin out of the body with epithelial stem cells. The method includes the following steps: (1) preparation of the epithelial stem cells: epithelial cells are made into cell suspension liquid, adhered and screened by IV collagen, and cultivated with epithelial stem cell media; (2) preparation of deepitheliarizing derma; (3) construction of the tissue engineering skin: epithelial stem cells for 2nd to 5th generation cultivation are selected, Dispase enzyme is adopted to digest the bossy cell patches in the size of rice to soya beam, the patches are inoculated to the deepitheliarizing derma, the tissue media is added, to cultivate the tissue engineering skin underwater or on the water. The tissue engineering skin constructed by the method of the invention is more suitable for the biological characteristics of human skin.

The invention relates to the field of biological medicine, and particularly provides an injection preparation of an anti-PD-L1 monoclonal antibody, which comprises the anti-PD-L1 monoclonal antibody with the protein content of 10-60 mg / ml; a buffer agent having a concentration of 10-60 mM; a stabilizer with the concentration of 100 to 150 mM; an osmotic pressure regulator with the concentration of10-100 mM; and 0.005%-0.05% (w / v) of a surfactant; wherein a pH value of the injection preparation is 5.8 to 6.3. The anti-PD-L1 monoclonal antibody disclosed by the invention has relatively high affinity and good biological activity; meanwhile, different auxiliary materials are selected according to the characteristics of the anti-PD-L1 monoclonal antibody, so that the safety and effectiveness of the monoclonal antibody in the preservation process can be guaranteed, and the physical stability, chemical stability and biological stability of the anti-PD-L1 monoclonal antibody can be maintained.

The invention discloses a tissue engineering urethra stent and a preparation technology thereof, and relates to the technical field of high molecular materials. Specifically, through the preparation technology, a tissue engineering urethra stent which is suitable for different individuals can be prepared, and through the preparation technology, large-scale batch production can be facilitated. Secondly, the tissue engineering urethra stent prepared through the preparation technology has favorable biocompatibility, favorable biodegradability and favorable bio-mechanical properties; and through the microcosmic porous structure of the tissue engineering urethra stent, adhesion and proliferation of seed cells can be guaranteed, and the tissue engineering urethra stent can carry the seed cells of large enough quantity, is suitable for urethra damage of different forms, and is particularly suitable for repair of long-segment urethra damage.

The present invention discloses a skin care product with the function of preventing air pollution and aims at providing a skin care product which can prevent various pollutions in the air from damaging the skin and keep the skin health. According to the weight percentage, the skin care product consists of 4 percent to 16 percent of composite protective active component and 84 percent to 93 percent of regular cosmetic basal component. The composite protective active component consists of anti-pollution factor, dustproof factor and ultraviolet absorption resistant agent and contains 3 percent to 8 percent of the anti-pollution factor, 0.5 percent to 3 percent of the dustproof factor and 0.5 percent to 5 percent of the ultraviolet absorption resistant agent according to the weight percentage. The composite protective active component of the skin care product of the present invention can bring the coordinate effect-increasing action into play well to achieve the purposes of face beautification and skin care by reasonable coordination and the selection of the dosage. The present invention can insulate the ultraviolet and at the same time can prevent and reduce the damage towards the skin caused by air pollution, which resists various kinds of pollution sources in the air completely to avoid using various kinds of protection articles synchronously and is convenient for using.

The invention discloses a culture method capable of promoting cyclocarya paliurus callus growth and secondary metabolite accumulation. The culture method includes: taking materials, and disinfecting;selecting semi-lignified stems with buds and top-end leaflets of cyclocarya paliurus, and disinfecting; performing callus induced culture: culturing stems and leaves in an induced culture medium to generate callus, wherein culture conditions are continuous dark treatment, and culture temperature is set at 20 DEG C; performing callus multiplication culture: transferring the callus into a transgenerational multiplication culture medium for transgenerational multiplication culture, and using a sealing film to sea, and putting into a culture chamber for culture, wherein culture conditions are continuous dark treatment, and culture temperature is set at 20 DEG C. Induction and multiplication culture of the callus are performed at temperature of 20 DEG C, so that generation of phenolic substances is inhibited while normal growth of cyclocarya paliurus is ensured, and callus growth and secondary metabolite accumulation are promoted at low temperature.

The invention provides a composition for oocyte and skin adult stem cell culture and applications thereof. The composition can improve the in-vitro maturation rate of oocyte, cleavage rate of ovum, and rate that embryo grows into mulberry and blastocyst; and thus the growth from in-vitro fertilized ovum to embryo is greatly promoted. At the same time, the differentiation from skin adult stem cells to fibroblast is effectively prohibited by the composition, or the self renewing and regeneration of skin adult stem cells are maintained. The composition comprises cysteamine, leukemia inhibitory factor, and Rho kinase inhibitor Y27632.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat microglial cells. The microglial cells are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat microglial cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat microglial cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat microglial cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention belongs to the technical field of biology, and particularly relates to endothelial cell culture solution. The endothelial cell culture solution comprises a basic culture medium DMEM (dulbecco modified eagle medium), human platelet lysate, L-glutamine, vitamin C, laminin 10, laminin 11, fibronectin, penicillin and streptomycin. The endothelial cell culture solution has the advantages that a pH (potential of hydrogen) value of the endothelial cell culture solution is 6.8-7.0, and the osmotic pressure of the endothelial cell culture solution is 310 mOsm / kg; endothelial cells cultured by the endothelial cell culture solution are high in adherence and proliferation speed, purity, migration capacity and lumen formation rate, invasion effects of the endothelial cells can be inhibited, and accordingly the endothelial cell culture solution is a perfect endothelial cell culture system.

The invention discloses an acipenser dabryanus spermatogonium culture solution and application thereof. According to the culture solution, a low-serum culture medium which does not have fetal calf serum, adopts various growth factors, nonessential amino acid, sodium pyruvate and other additives as substitutes and is assisted by a small amount of male acipenser dabryanus serum is provided so as to guarantee mass reproduction of spermatogonium and low reproduction rate of a spermatic cell; and the spermatic cell 13d which is cultured to the 13rd day are doped with 25mu M BrdU and is marked as a newly reproduced cell. 24h immunohistochemical experiments indicate that the ratio of germ cell marked cells in newly reproduced cells after BrdU is doped for 24 hours is 85.78 percent. According to the culture solution, the current problem that the acipenser dabryanus spermatogonium passage cannot be massively cultured can be solved, and an effective cell platform is provided to acipenser dabryanus reproductive development researches.

The invention discloses a multi-stage temperature-control ventilation production method of multi-microorganism high-temperature bacterial fragrant koji, and belongs to the technical field of wine brewing. The method comprises the following steps: separately culturing high-temperature pure bacterial liquids, carrying out two-stage amplification culture to obtain a multi-microorganism high-temperature bacterial liquid, then carrying out multi-stage temperature control continuous ventilation or intermittent ventilation culture, and carrying out drying to obtain the multi-microorganism high-temperature bacterial fragrant koji. Advanced multi-microorganism high-temperature bacteria are heated actively and quickly in a brand-new temperature control mode, microbial contamination is effectively avoided, meanwhile, the multi-stage gradient heating and ventilation mode ensures the temperature rise adaptability of effective microbial communities, and proliferation of bacteria and generation and accumulation of metabolic flavor substances are ensured; the produced multi-microorganism high-temperature bacterial fragrant koji is rich in Maotai-flavour and scorch aroma and typical in aroma, and can be used for producing soft and other types of liquor seasoning wines, and the economic benefit is improved.

The invention relates to the field of biological medicine, and particularly provides an injection preparation of an anti-PD-L1 monoclonal antibody, which comprises the anti-PD-L1 monoclonal antibody with the protein content of 10-60 mg / ml; a buffer agent having a concentration of 10-60 mM; a stabilizer with the concentration of 100 to 150 mM; an osmotic pressure regulator with the concentration of10-100 mM; and 0.005%-0.05% (w / v) of a surfactant; wherein a pH value of the injection preparation is 5.8 to 6.3. The anti-PD-L1 monoclonal antibody disclosed by the invention has relatively high affinity and good biological activity; meanwhile, different auxiliary materials are selected according to the characteristics of the anti-PD-L1 monoclonal antibody, so that the safety and effectiveness of the monoclonal antibody in the preservation process can be guaranteed, and the physical stability, chemical stability and biological stability of the anti-PD-L1 monoclonal antibody can be maintained.

The invention discloses a bionic three-dimensional DPCs independent co-culture system and a construction method thereof. The construction method comprises the following steps: preparing single-cell suspensions of DPCs, DSCs and HGMCs; providing Matrigel, adding the DPCs single-cell suspension into the Matrigel, and carrying out resuspension to form a Matrigel-DP cell suspension; enabling the Matrigel-DP cell suspension to be adhered to a microporous membrane of a Transwell cell, so as to prepare a three-dimensional DPCs culture system; inoculating HGMCs in an upper chamber of the Transwell cell of the three-dimensional DPCs culture system, simultaneously inoculating DSCs in a lower chamber, and then adding a culture medium to prepare the bionic three-dimensional DPCs independent co-culturesystem. The in-vivo microenvironment of the DPCs can be effectively simulated, the difference between in-vitro cultured DPCs and in-vivo normal DPCs is reduced, and in-vitro long-time maintenance ofbiological functions of the DPCs is realized.

The invention discloses a brewing method of originally brewed low-alcohol raspberry beer. According to the brewing method of the raspberry beer, provided by the invention, raw materials including raspberry juice and original wheat juice are adopted, and the beer is brewed by fermenting the raw materials by using Leid yeast; the alcoholic strength of the raspberry beer is smaller than or equal to 2.5% vol, the sugar content of the raspberry juice is larger than or equal to 300 g / L, and the sugar content of the original wort is 6.0 + / -0.5 degree P. According to the method, the alcoholic strength of the raspberry beer can be effectively controlled, the sour-sweet balance degree of the finished wine is adjusted, and the foam retention of the product is guaranteed. 100L large-scale production verifies that the raspberry beer obtained by the invention has the alcoholic strength of less than or equal to 2.5% vol, and is clear and transparent in appearance, rich in raspberry fragrance, moderate in taste, harmonious in mouth, sour and refreshing in mouth closing, free of other foreign flavors and suitable for being eaten together with rice or bread.

The invention discloses a brewing method for brewing originally brewed low-alcohol blackcurrant beer. According to the method for brewing the blackcurrant beer, blackcurrant juice and mixed wort are used as raw materials, and the beer is brewed by fermenting the blackcurrant juice and the mixed wort by using saccharomyces. The alcoholic strength of the blackcurrant beer is smaller than or equal to 2.5% vol, the sugar content of the blackcurrant juice is larger than or equal to 300 g / L, and the sugar content of the barley wort is 5.5-6.5 DEG P. The blackcurrant beer brewed by the method disclosed by the invention is standard purple black in appearance, strong in blackcurrant fragrance, strong in taste, harmonious in mouth feel, moderate in alcohol warm feeling, typical in blackcurrant herbal fragrance, free of other foreign flavors and suitable for being eaten together with rice or bread.

The invention discloses a skin-care product with an air pollution prevention function and aims at providing a skin-care product which can prevent injuries, caused by various pollution in the air, to skin and can keep the skin healthy. The skin-care product is prepared from the following components in percentage by weight: 4 percent to 16 percent of a compound protective active ingredient and 84 percent to 93 percent of a conventional cosmetic basic component, wherein the compound protective active ingredient is prepared from the components in percentage by weight: 3 percent to 8 percent of anti-pollution factor, 0.5 percent to 3 percent of dustproof factor and 0.5 percent to 5 percent of anti-ultraviolet absorbent. According to the skin-care product disclosed by the invention, the compound protective active ingredient is reasonably matched and the dosage is reasonably selected, so that the synergistic interaction effect can be extremely expressed and the aims of beautifying and protecting skin are realized. When the skin-care product is used for isolating ultraviolet rays, the injuries, caused by air pollution, to the skin can be prevented and reduced and various pollution sources in the air are comprehensively resisted; a condition that various types of protection articles are used simultaneously is avoided and the skin-care product is convenient to use.

The invention discloses a double-layer artificial skin culture device and a use method thereof. The device comprises a culture box and a sealing cover, wherein the culture box is internally provided with a telescopic rod and a culture stand; one end of the telescopic rod is mounted on the bottom plate of the culture box; and the other end of the telescopic rod is mounted on the culture stand. Theuse method of the double-layer artificial skin culture device comprises the following steps: compressing the pressing rod to enable the culture stand to fit to the bottom plate of the culture box; placing a layer of a nylon membrane on the culture stand; adding a layer of a cell-free collagen on the nylon membrane; inoculating dermal fibroblasts, adding a culture liquid, covering the components bya sealing cover, and performing culture so as to form a dermis layer; opening the sealing cover, inoculating epidermal cells on the dermis layer, covering the components by the sealing cover, and culturing the epidermal cells so as to form an epidermal layer; inserting a fixing column into a fixing hole in the bottom of the culture box, jacking up the culture stand, enabling the top of the epidermal layer to be in contact with the air, and cutinizing the epidermal cells, so as to obtain double-layer artificial skin.

The invention belongs to the technical field of citrus processing, and relates to a preparation method of a citrus lactobacillus capsule with high flavone utilization degree, the method comprises the following steps: (1) raw material treatment: crushing a dried citrus peel raw material, adding soaked rice, stirring uniformly, steaming in a waterproof manner, and cooling; (2) adding koji for saccharification, namely adding sweet wine koji into the citrus peel raw material cooled to the saccharification temperature while the citrus peel raw material is hot, uniformly stirring, and saccharifying at constant temperature; (3) heating for enzyme deactivation: after saccharification is finished, heating the citrus peel saccharified substance to 65 DEG C or above, and cooling after enzyme deactivation; (4) adding lactobacillus: adding the activated lactobacillus in the RMS liquid culture medium into the orange peel saccharified substance cooled to the room temperature, uniformly stirring, and performing semi-solid constant-temperature fermentation in a sealed state; (5) squeezing and separating; and (6) post-treatment. According to the preparation method of the citrus lactobacillus capsule with high flavone availability, provided by the invention, the bioavailability of a citrus flavone product is improved, and meanwhile, an intestinal health-care effect is taken into account.

The invention belongs to the field of culture mediums, and particularly relates to a serum-free chemical component definition culture medium for EB66 cell strain suspension growth. The culture mediumcomprises an additive A including, according to the concentrations of the substances in the culture medium, 1-10 mg / L of insulin, 5-10 microgram / L of IGF-1, 0.5-2 mg of putrescine, 0.5-5 mg / L of glutathione, 1-5 mg / L of neovaricaine, 10-50 micromole of beta-mercaptoethanol, 1-5 mg / L of lecithin, 10-50 mg / L of vitamin C, 50-100 mg / L of taurine, 2-5 mg / L of cholesterol and 3-6 millimole of glutamine, wherein 1000-3000 mg of an amino acid mixed component is added to per liter of the culture medium. The culture medium supports rapid proliferation of EB66 cells under the serum-free and full-suspension conditions, and the doubling time is short. The cells are high in density. Besides, the invention further provides a preparation method of the culture medium.

A method for inducing embryonic calluses of large-fruit camellia chekiangoleosa comprises the following steps of (1) selecting tender shoots on newly-drawn branches in the same year as explants, carrying out disinfection treatment, and cutting off old wounds for later use; 2) inoculating the explant subjected to disinfection treatment in the step 1) into an induction culture medium for induction culture for 3-4 weeks to obtain the embryonic calluses; and 3) transferring the embryogenic callus obtained in the step 2) into a proliferation culture medium for proliferation culture, and performingsubculture once every three weeks to obtain a large number of embryogenic tissues. The method for inducing is convenient and simple to operate, can quickly achieve induction and proliferation to obtain the large number of embryogenic calluses, and provides a basis for industrial production of the large-fruit camellia chekiangoleosa.

The invention discloses a long-term multiplication and storage method for embryonic callus of sweet potatoes. The method comprises the processes of selection of successive embryonic callus, successive multiplication of the embryonic callus, and induction of the embryonic callus to mature embryo regeneration. The method has the advantages that: the process flow is simple, the multiplication rate of the embryonic callus is high, and the successive time is long; the technical problem that the long-term utilization of the embryonic callus is limited because the materials are limited by seasons and the embryonic retaining time is short and the like in the sweet potato tissue culture process is solved; a good receptor material is provided for the transgene technology of the sweet potatoes; and a large amount of efficient regenerated material is provided for annually producing the tissue culture seedlings of the sweet potatoes.

The present invention relates to a chimeric antigen receptor (CAR), especially a dual-signal independent chimeric antigen receptor, and also relates to an immune response cell expressing the CAR, and the immune response cell is used for preparing and treating malignant tumors and Use in medicine for viral infectious diseases. Specifically, the dual-signal-independent CAR recognizes antigens from two different families of tumor cells, respectively, and transmits two signals related to T cell activation. One of the CARs transmits the first signal related to T cell activation by binding to tumor-specific antigens or ligands of tumor-associated antigens, which determines the killing specificity of T cells; the other CAR binds to membrane receptors widely expressed by tumor cells (such as The ligand of EGFR family member protein) transmits the second signal related to T cell activation and promotes T cell activation, proliferation and survival. The present invention can avoid the potential safety problems on the basis of maintaining the curative effect of the second-generation and third-generation CARs.

The invention provides a nitrogen and phosphorus treatment drainage channel structure for agricultural non-point source pollution treatment, and relates to the field of drainage channels. According to the nitrogen and phosphorus treatment drainage channel structure for agricultural non-point source pollution treatment, the nitrogen and phosphorus treatment drainage channel structure comprises a first section and a second section, a filter box is arranged between the first section and the second section, a prefabricated bottom plate is laid at the bottom of the nitrogen and phosphorus treatment drainage channel structure, and cement baffles are arranged on the two sides of the prefabricated bottom plate; grass planting bricks are laid on side slopes on the two sides of the drainage channel structure for nitrogen and phosphorus treatment, a first porous medium is laid between the two cement baffles in the first section, cobblestones are laid on the first porous medium, ceramsite is further laid on the second section on the basis of the first section, and the first porous medium and the second porous medium are laid on the basis of the second section. Second porous media are laid on the lower portion of the side slope of the drainage channel structure for nitrogen and phosphorus treatment and the two sides of the cobblestones. By adopting the modes of plant planting and microbiological treatment, the water channel has the purification capacity.

The invention discloses a multifunctional stem cell culture medium, and belongs to the technical field of bioengineering. The multifunctional stem cell culture medium comprises a basic culture mediumand the following added components: 150-200 microliters / cm<2> of Kangning BD Matrigel, 10-20 nanograms / mL of sodium selenite, 20-200 nanograms / mL of recombinant transferrin, 20-30 micrograms / mL of insulin, 400-600 micrograms / mL of allicin and 40-60 micrograms / mL of streptomycin. Due to adoption of the Kangning BD Matrigel, the Matrigel is polymerized to form a three-dimensional matrix with biological activity, the structure, the composition, the physical properties and the functions of an in-vivo cell basement membrane are simulated, and culture and differentiation of in-vitro cells are facilitated; under the action of the recombinant transferrin, the insulin and the like, normal proliferation of cells can be ensured, and the possibility of pathogen contamination is reduced; and due to combined use of the allicin and the streptomycin, the inhibition and killing functions of the allicin to bacteria can be remarkably improved, the cell culture medium disclosed by the invention has goodinhibition and killing functions to bacteria, growth and proliferation of cells are facilitated, and the possibility of bacterial infection in the cell culture medium is reduced.

The invention relates to a construction method of a barrier function weakening model. The construction method comprises the following steps: step 1, preparation of cells; step 2, inoculation and culture of the barrier function weakening model under liquid; step 3, gas-liquid surface proliferation and differentiation culture of the barrier function weakening model; and step 4, gas-liquid surface differentiation inhibition culture of the barrier function weakening model. The barrier function weakening model can well replace an animal model, becomes a core tool for in-vitro testing, and can be applied to in-vitro testing of safety and efficacy of products such as infant skin care products, cosmetics with human skin barrier repairing efficacy and the like.