Double-layer artificial skin culture device and use method thereof

Abstract

The invention discloses a double-layer artificial skin culture device and a use method thereof. The device comprises a culture box and a sealing cover, wherein the culture box is internally provided with a telescopic rod and a culture stand; one end of the telescopic rod is mounted on the bottom plate of the culture box; and the other end of the telescopic rod is mounted on the culture stand. Theuse method of the double-layer artificial skin culture device comprises the following steps: compressing the pressing rod to enable the culture stand to fit to the bottom plate of the culture box; placing a layer of a nylon membrane on the culture stand; adding a layer of a cell-free collagen on the nylon membrane; inoculating dermal fibroblasts, adding a culture liquid, covering the components bya sealing cover, and performing culture so as to form a dermis layer; opening the sealing cover, inoculating epidermal cells on the dermis layer, covering the components by the sealing cover, and culturing the epidermal cells so as to form an epidermal layer; inserting a fixing column into a fixing hole in the bottom of the culture box, jacking up the culture stand, enabling the top of the epidermal layer to be in contact with the air, and cutinizing the epidermal cells, so as to obtain double-layer artificial skin.

Description

technical field [0001] The invention belongs to the technical field of medical devices, in particular to a double-layer artificial skin culture device and its application method. Background technique [0002] With the development of tissue engineering technology, different types of tissue-skin substitutes have been developed successively, which can be used clinically for transplantation treatment of skin defects. The preparation method of tissue-engineered skin includes the following steps: inoculation of dermal cells, It is cultivated to form a dermis, then inoculated epidermal cells on the dermis, cultivated to form an epidermis, and finally keratinized. The keratinization step makes the obtained tissue-engineered full-thickness skin closer to natural skin and can resist infection, so this step is necessary. The degree of keratinization of the epidermis directly affects the quality and use of the obtained tissue-engineered full-thickness skin. In the prior art, by exposin...

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Problems solved by technology

[0003] In the prior art, in the keratinization step of tissue-engineered full-thickness skin, an air-liquid interface is formed by controlling the amount of culture solution added, and the surface epidermal cells are exposed to the air, while dermal cells and other epidermal cells are in contact with the culture solution; In this process, adding less amount of culture solution will affect the proliferation of cells, adding too much culture solution will affect the contact area between epidermal cells and air, resulting in incomplete keratinization, too thin keratinized layer, etc., which will eventually affect artificial skin quality

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