33 results about "Naked mole-rat" patented technology

The invention relates to the field of in-vitro culture of cells and in particular relates to an isolated culture method of alveolar type II epithelial cells of naked mole rats. The isolated culture method comprises the following steps: combining pancreatin and elastase and carrying out digestion and isolation; filtering, carrying out differential adhesion and screening and purifying an IgG antibody to obtain the alveolar type II epithelial cells of the naked mole rats. The invention establishes one set of method for isolating, culturing and identifying the alveolar type II epithelial cells of the naked mole rats; a biological characteristic maintaining condition of the cells cultured by the method is evaluated, so that the culture method used for obtaining the high-purity alveolar type II epithelial cells of the naked mole rats is verified, the possibility for further deeply researching proliferation and differentiation, liquid transferring, synthesis and secretion of the alveolar type II epithelial cells, and function changes of the alveolar type II epithelial cells under pathophysiologic conditions of lung injuries and repairing and the like is provided.

A conditioned cell culture medium of cells derived from Spalax or naked mole rat (Heterocephalus glaber) and methods for preparing it are provided. Pharmaceutical compositions comprising the conditioned cell culture medium and its use in the treatment of cancer as well as methods for identifying anti-cancer agents are also provided.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat oligodendroglia precursor cells. The oligodendroglia precursor cells are separated from cerebral cortices of naked mole rat fetal rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat oligodendroglia precursor cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat oligodendroglia precursor cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat oligodendroglia precursor cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention relates to the technical field of cell biology, in particular to a separation, purification and culture method of naked mole rat hippocampal neurons. The hippocampal neurons are separated from newly-born naked mole rat cerebral cortices and purified and cultured by the aid of multiple culture media comprehensively, and a reasonable culture method suitable for poikilothermal rodent mammal naked mole rat hippocampal neurons is found out. With the adoption of the method, a large number of naked mole rat hippocampal neurons with normal functional activity can be acquired conveniently, efficiently and economically, the cells can still keep biological characteristic in the in-vitro state in the in-vitro environment through culture under the low-oxygen condition, so that special physiological functions of the naked mole rat hippocampal neurons are further researched in pure in-vitro cell culture models directly and conveniently, and the important theoretical basis is provided for exploration of the biological mechanism and application to related clinical fields.

The invention relates to the field of stem cell culture, and particularly discloses a primary separation culture method for bone marrow mesenchymal stem cells of naked mole rats. The primary separation culture method includes steps of 1), washing bone marrow of the naked mole rats; 2), sieving bone marrow washed substances by the aid of cell strainers; 3), breaking red blood cells; 4), carrying out direct plate laying. The primary separation culture method for the bone marrow mesenchymal stem cells of the naked mole rats has the advantages that the primary separation culture method is established for the bone marrow mesenchymal stem cells of the naked mole rats, biological characteristics of the bone marrow mesenchymal stem cells cultured by the aid of the primary separation culture method are evaluated, the fact that the bone marrow mesenchymal stem cells with extremely high purity and differentiation capacity can be obtained by the aid of the primary separation culture method is proved, and accordingly intensive study on characteristics of stem cells of the naked mole rats and exploration on characteristics of somatic cells, which are difficult to separate but can be obtained from the stem cells by means of transformation, of the naked mole rats can be possibly carried out.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat astroglia cells. The astroglia cells are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat astroglia cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat astroglia cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat astroglia cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

This invention provides cell culture compositions which produce significant quantities of high molecular weight hyaluronic acid. The cell cultures are obtained from cells of mole rats, such as naked mole rats and blind mole rats. The high molecular weight hyaluronic acid can be collected in the conditioned media of these cell cultures. These cell cultures provide a convenient source of large quantities of high molecular weight hyaluronic acid.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat microglial cells. The microglial cells are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat microglial cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat microglial cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat microglial cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention relates to the field of cell cultivation and in particular relates to a separation and purification method of testicular interstitial cells of a naked mole rat. The interstitial cells of the naked mole rat are separated and purified by adopting enzymatic digestion and a Percoll continuous density gradient method. The method provided by the invention can obtain a lot of testicular interstitial cells of the naked mole rat with normal functional activity simply, efficiently and economically, and cultivation under a low oxygen condition can ensure that the cells still can keep the biological characteristics in an in-vivo state in an in-vitro environment, so that the special physiological functions of the testicular interstitial cells of the naked mole rat can be conveniently and directly researched in a pure in-vitro cell cultivation model further, thereby providing an important theoretical basis for exploring the biological mechanism and applying the same to the clinical related fields.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat cortical neurons. The cortical neurons are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat cortical neurons is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat cortical neurons with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat cortical neurons can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention relates to the field of experimental animal production, and in particular relates to a superovulation method of naked mole rats. The superovulation method comprises the following steps of: at the 1st, 3th, 5th and 7th days after the oestrus is ended, injecting PMSG (Pregnant Mare Serum Gonadotropin) into the abdominal cavities of the female naked mole rats; at the 7th day, injecting HCG with the dosage of 0.12IU / g (body weight) into the abdominal cavities of the naked mole rats; at the time of 1 hour after injection of HCG, starting to cage the female naked mole rats and male rats with fertility for free mating. The superovulation method determines the implementation schedule, the drug ingredients and dosage by verification of numerous tests. The superovulation method has the advantages that a set of superovulation program for the naked mole rats is made, so that the blank that the superovulation program is not made temporarily after introduction of the naked mole rats in China is filled; every experimental animal responses to the superovulation and can form 51-55 follicles. The adopted drugs and program are more suitable for the naked mole rats, and the superovulation program is more economical.

The invention relates to the technical field of cytobiology, in particular to a naked mole rat DRG neuron culture method. DRG neurons are separated from embryonic naked mole rat medulla spinalis, purified and cultured by comprehensively adopting multiple culture media, and then the reasonable culture method suitable for the DRG neurons of poikilothermal rodent mammal naked mole rats is explored. According to the method, a large quantity of naked mole rat DRG neurons with the normal functional activity can be easily, conveniently, efficiently and economically obtained, it can be guaranteed that the cells still can keep the biological characteristics achieved under the body condition in an in-vitro environment by means of culture conducted under anoxic condition, then the special physiological functions of the naked mole rat DRG neurons can be conveniently further researched in a pure in-vitro cell culture environment, and an important theory basis is provided for exploration of the biological mechanism of the naked mole rat DRG neurons and application of the naked mole rat DRG neurons in the clinical related fields.

The invention belongs to the technical field of cell culture, and particularly relates to a culture method for a heterocephalus glaber cardiac muscle cell. In the culture method, the operation steps of separating and purifying the heterocephalus glaber cardiac muscle cell are simple, cell attachment is fast, the survival rate is high, and a cell culture system is high in stability. The culture method is an ideal culture method for the heterocephalus glaber primary cardiac muscle cell, can basically meet the requirements for heterocephalus glaber cardiac muscle cell related experiment research in all research fields such as medical science, biology, pharmacy and bioscience and is suitable for application and popularization in general laboratories.

The invention relates to the technical field of cell biology, in particular to a method for separating, purifying and culturing naked mole-rat Schwann cells. The invention comprehensively uses various mediums to separate and purify Schwann cells from the DRG ganglion of the spinal cord of naked mole rat fetal rats, and finds out a reasonable culture method for naked mole rat Schwann cells suitable for temperature-changing rodent mammals. The method of the present invention can obtain a large number of naked mole-rat Schwann cells with normal functional activity in a simple, efficient and economical manner, and the culture under hypoxic conditions can ensure that the biological characteristics of the cells in the in vitro environment can still be maintained. , so that it is convenient to further study the special physiological functions of naked mole rat Schwann cells directly in a pure in vitro cell culture model, thereby providing an important theoretical basis for exploring the biological mechanism and applying it to clinical related fields.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat astroglia cells. The astroglia cells are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat astroglia cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat astroglia cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat astroglia cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention relates to the field of in vitro culture of cells, in particular to a method for separating and culturing naked mole-rat skeletal muscle myoblasts. The naked mole-rat is obtained by using trypsin combined with collagenase and neutral protease to digest and separate, and filtering and differentially adhering to the wall Skeletal muscle myoblasts. The present invention establishes a set of methods for isolating, cultivating and identifying naked mole-rat skeletal muscle myoblasts, and evaluates the maintenance of the biological characteristics of the cells cultured by this method, confirming that this culture method can obtain naked mole rat skeletal myoblasts, which provides the possibility to further study the functional changes of naked mole rat skeletal myoblasts under hypoxic physiological conditions.

The invention relates to the technical field of animal breeding and specifically relates to a method for breeding and pairing naked mole rats. According to the method for breeding and pairing the naked mole rats, provided by the invention, from the weight pairing for the parent naked mole rats to the selection for the mating time and from the single cage breeding before the mating to the body odor mixing, a series of methods for increasing the success rate of the mating of the naked mole rats are formed. These methods can be used for overcoming the mating barrier of the naked mole rats and can supply key technical support for establishing a closed group of the naked mole rats.

The invention relates to a naked mole-rat heath protection, longevity, health care wine and a preparation method thereof. The health care wine comprises following raw materials: naked mole-rat polymer hyaluronic acid mixed solution, ginseng, polygonatum rhizome, raspberry, poria cocos, arborvitae seed, semen astragali complanati, hovenia dulcis thunb, radix ophiopogonis, cyathula root, fried Chinese yam, fried gorgon fruit, evening primrose, raw rehmannia root, longan aril, walnut pulp, and wolfberry. The abovementioned raw materials are added into a pure cereal wine, and then the wine is stored in a shade place for a month to obtain the naked mole-rat heath protection, longevity, health care wine. The naked mole-rat heath protection, longevity, health care wine can inhibit tumor, enhance the immunity, tonify essence and marrow, and prominently enhance the fluid immunity and cell immunity of organisms, and is suitable for following symptoms: bad resistance to wind cold and labor due to weak physique, qi and blood deficiency caused by worry beyond measure, hemiplegia, numbness of hands and feet, coldness, and the like.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat cortical neurons. The cortical neurons are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat cortical neurons is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat cortical neurons with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat cortical neurons can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention relates to the field of experimental animal production, and in particular relates to a superovulation method of naked mole rats. The superovulation method comprises the following steps of: at the 1st, 3th, 5th and 7th days after the oestrus is ended, injecting PMSG (Pregnant Mare Serum Gonadotropin) into the abdominal cavities of the female naked mole rats; at the 7th day, injecting HCG with the dosage of 0.12IU / g (body weight) into the abdominal cavities of the naked mole rats; at the time of 1 hour after injection of HCG, starting to cage the female naked mole rats and male rats with fertility for free mating. The superovulation method determines the implementation schedule, the drug ingredients and dosage by verification of numerous tests. The superovulation method has the advantages that a set of superovulation program for the naked mole rats is made, so that the blank that the superovulation program is not made temporarily after introduction of the naked mole rats in China is filled; every experimental animal responses to the superovulation and can form 51-55 follicles. The adopted drugs and program are more suitable for the naked mole rats, and the superovulation program is more economical.

The invention relates to the technical field of animal breeding and specifically relates to a method for breeding and pairing naked mole rats. According to the method for breeding and pairing the naked mole rats, provided by the invention, from the weight pairing for the parent naked mole rats to the selection for the mating time and from the single cage breeding before the mating to the body odor mixing, a series of methods for increasing the success rate of the mating of the naked mole rats are formed. These methods can be used for overcoming the mating barrier of the naked mole rats and can supply key technical support for establishing a closed group of the naked mole rats.

The invention belongs to the field of biological technical detection, and particularly relates to a naked mole rat microsatellite marker site, a primer and application thereof. The number of the built naked mole rat microsatellite marker sites reaches 45; the naked mole rat microsatellite marker sites can be used for genetic polymorphism detection of naked mole rat colonies; firm foundation is laid for naked mole rat microsatellite polymorphism detection.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat oligodendroglia precursor cells. The oligodendroglia precursor cells are separated from cerebral cortices of naked mole rat fetal rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat oligodendroglia precursor cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat oligodendroglia precursor cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat oligodendroglia precursor cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat microglial cells. The microglial cells are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat microglial cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat microglial cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat microglial cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

A conditioned cell culture medium of cells derived from Spalax or naked mole rat (Heterocephalus glaber) and methods for preparing it are provided. Pharmaceutical compositions comprising the conditioned cell culture medium and its use in the treatment of cancer as well as methods for identifying anti-cancer agents are also provided.