143 results about "Schwann cell myelin sheath" patented technology

A neural regeneration conduit employing spiral geometry is disclosed. The spiral geometry is produced by rolling a flat sheet into a cylinder. The conduit can contain a multiplicity of functional layers lining the lumen of the conduit, including a confluent layer of adherent Schwann cells. The conduit can produce a neurotrophic agent concentration gradient by virtue of neurotrophic agent-laden microspheres arranged in a nonuniform pattern and embedded in a polymer hydrogen layer lining the lumen of the conduit.

Three novel low molecular weight (LMW) polypeptide fragments of a proteolipid protein human PLP / DM20 are designated PIRP-M, PIRP-L and PIRP-J, and are growth factors for oligodendrocytes with anti-apoptotic activity. They are encoded by mRNA from an IRES. Fusion polypeptides of such a LMW polypeptide, DNA encoding the LMW polypeptide and fusion polypeptide, expression vectors comprising such DNA, and cells expressing such polypeptides, or pharmaceutical compositions thereof, are useful for stimulating neural stem cell differentiation, maturation along the oligodendrocytic pathway and proliferation of oligodendrocytes or precursors. These compositions can protect oligodendrocytes (and nonneural cells) from apoptotic death. Thus, the present composition is used to treat a disease or condition in which such differentiation, maturation and proliferation or inhibition of cell death, including remyelination or stimulation of oligodendroglia or Schwann cells, is desirable. Disorders include multiple sclerosis, trauma with Parkinson's-like symptoms, hypoxic ischerriia and spinal cord trauma.

There is provided a method of inducing bone marrow stromal cells to differentiate into bone marrow stromal cell-derived Schwann cells in vitro, comprising the steps of: collecting bone marrow stromal cells from bone marrow and culturing the cells in a standard essential culture medium supplemented with a serum; adding a reducing agent to the culture medium and further culturing the cells; adding a differentiation inducing agent to the culture medium and further culturing the cells; and adding a cyclic AMP-augmenting agent or a cyclic AMP analogue and / or a glial cell differentiation and survival stimulating factor to the culture medium, and further culturing the cells to obtain the bone marrow stromal cell-derived Schwann cells. There are also provided bone marrow stromal cell-derived Schwann cells obtained thereby and a pharmaceutical composition for neural regeneration that comprises them.

The invention discloses a chitosan artificial nerve graft containing a neurotrophic factor and a preparation method thereof. In the chitosan artificial nerve graft, chitosan is processed into a nerve conduit with a porous structure and high tensile strength by performing processes such as weak acid dissolving, injection molding, molding, neutralization fixing, cleaning, freeze drying and the like under the condition of not adding any foaming agent or crosslinking agent. The nerve conduit contains cells or tissues which have treating effects and are included or distributed inside or on the surfaces of tubular body pores, including autologous bone marrow stem cells, autologous bone mesenchymal stem cells, schwann cells or dorsal root ganglion tissues or combinations thereof. A tissue engineering nerve can be used for repairing peripheral nerve defect and can be applied to repairing of spinal cord injury simultaneously.

The invention discloses a multi-channel nerve repair conduit with a tissue induced function and a mold; chitosan-coated salidroside microspheres and composite type salidroside slow-release microspheres are prepared, the drug cumulative release amount is calculated, the composite type salidroside slow-release microspheres with different contents are mixed with I-type collagen, and salidroside slow-release microspheres / I-type collagen is obtained; a multi-aperture cylindrical nerve conduit core layer is prepared by using the mold, a nano fiber nerve conduit shell layer is prepared by a high-pressure electrostatic spinning technology, the core layer and the shell layer are nested, and thus the multi-channel nerve repair conduit with the tissue induced function is prepared. The shell layer of the conduit has a good role in exchanging with a tissue fluid, and has a function of guiding nerve growth; the core layer has the functions of guiding nerve fiber orientation growth, promoting stem cells to directionally differentiate to schwann cells and accelerating the nerve fiber growth and function recovery, effectively repairs peripheral nerve defects, has good degradation and biocompatibility, and meets the requirements of a tissue engineering scaffold material.

Three novel low molecular weight (LMW) polypeptide fragments of a proteolipid protein human PLP / DM20 are designated PIRP-M, PIRP-L and PIRP-J, and are growth factors for oligodendrocytes with anti-apoptotic activity. They are encoded by mRNA from an IRES. Fusion polypeptides of such a LMW polypeptide, DNA encoding the LMW polypeptide and fusion polypeptide, expression vectors comprising such DNA, and cells expressing such polypeptides, or pharmaceutical compositions thereof, are useful for stimulating neural stem cell differentiation, maturation along the oligodendrocytic pathway and proliferation of oligodendrocytes or precursors. These compositions can protect oligodendrocytes (and nonneural cells) from apoptotic death. Thus, the present composition is used to treat a disease or condition in which such differentiation, maturation and proliferation or inhibition of cell death, including remyelination or stimulation of oligodendroglia or Schwann cells, is desirable. Disorders include multiple sclerosis, trauma with Parkinson's-like symptoms, hypoxic ischerriia and spinal cord trauma.

The invention belongs to the field of cell biology and relates to a method for inducing differentiation from human umbilical cord mesenchymai stem cells (hucMSCs) into neural cells. The method comprises that HUCMSCs and Schwann cells are putted in Transwell with apertures of 0.4 microns to be co-cultured separately; a Schwann cell culture medium is utilized as a cell culture medium and half or all of the cell culture medium is replaced every three days; and then neural cells can be obtained after two weeks of culturing. Through a separate co-culturing method utilized by the invention, an umbilical cord can be digested by a mixture of composite collagenase NB4 and hyaluronidase and thus a large amount of HUCMSCs can be separated. High purity HUCMSCs can be obtained in third generation passage cells. In the invention, HUCMSCs are induced and differentiate into neural cells, wherein a cell differentiation rate is over 70%. Expressing rate ranges of specific markers, such as NF-200, nestin, Betar-III-tubulin, etc., of neural cells are from 70 to 80%.

Methods and compositions are provided for lineage predetermination of cellular transplants including through liposome mediated transfection with aqueous protein extracts from populations of differentiated mammalian cells, or cellular fractions thereof, wherein the differentiated mammalian cells are enriched in one or more of adipocytes, chondrocytes, endothelial cells, hepatocytes, cardiomyocytes, smooth muscle cells, skeletal muscle cells, cardiac pacemaker cells, Schwann cells, pancreatic islet cells, hematopoietic cells, myeloblasts, neurons, and osteoblasts.

The invention discloses preparation and application of a nerve tissue matrix derived tissue engineering scaffold material. A nerve tissue is taken as a raw material, matrix components (including nano-scale collagen microfilaments, fibronectin microfilaments and laminin microfilaments) favorable for nerve regeneration are extracted by means of medicine expansion, mechanical pulverization, enzymolysis treatment, dialysis collection and the like, and immunogenicity components (including Schwann cells, phospholipid and axons) not favorable for nerve regeneration are removed. The prepared nerve tissue matrix derived material can be further prepared into a three-dimensional porous oriented scaffold through oriented crystallization, freeze drying and crosslinking separately or by matching other polymer materials, or is prepared into a nano-scale film by an electrospinning technology, and the film is wound to form a nerve regeneration catheter. The tissue engineering scaffold or catheter prepared by the method is favorable for adhesion, proliferation and migration of seed cells, promotes nerve generation and can be used for never defect repair.

The invention discloses a preparation method of a bionic artificial nerve scaffold established by a collagen. The method comprises the following steps: firstly preparing the collagen in three different shapes for later use: 1) dissolving the frozen and dried collagen by using hexafluoroisopropanol and preparing a nanofiber thin film by using an electrospinning technique; 2) preparing a linear scaffold containing an ordered fine pipeline by the collagen by combining freezing and drying with a mechanical stretching technology; and 3) dissolving the collagen in pure water to prepare a hydrogel; then, coiling the nanofiber thin film into a tube to simulate perilemma, wherein the ordered pipeline linear scaffold is filled in the tube to simulate perineurium; and finally, filling the hydrogel in the pipeline of the linear scaffold and the gap of the scaffold to simulate endoneurium. The bionic artificial nerve scaffold based on a bionic theory better simulates the structure of peripheral nerves and can promote and guide axon and Schwann cells to grow orderly and pass through damaged peripheral nerves based on a good mechanical property.

The invention discloses a new kind of nerve allotransplant, the preparation method and its medical application. The invention employs chemical extraction method to remove the schwann cell, axon and myelin sheath that can cause rejection for nerve allotransplant, stores stroma cell and three-dimensional pipeline structure for axon regeneration, and provides intact regenerative frame. The physical and biochemical property of nerve allotransplant are similar to that of normal nerve, and can be used as nerve allotransplant for damaged nerve.

The invention relates to a construction method of a porous gelatin sponge cylinder bracket material used for repairing nerve injury, in particular to a construction method of a stem cell-containing porous gelatin sponge cylinder bracket and an application thereof. When in use, the porous gelatin sponge cylinder bracket planted with stem cells and noblecellsor thereof / and schwann cells is transplanted to the injury part of complete transaction or hemitransection spinal cord, which can better promote injured nervus centralis regeneration and function reparation. The invention has important significance in strengthening injured neuron protection after spinal cord trauma in biological tissue engineering level, promoting axonal regeneration of injured neuron, improving quality of life of the sick and injured, reducing the heavy burdens of society and families, and promoting socioeconomic development of China.

It is an object of the present invention to provide a cell-containing composition capable of suppressing the outflow of the cells after transplantation and improving the survival rate of the cells. The present invention provides a composition which comprises any of bone marrow stromal cell-derived neural precursor cells, bone marrow stromal cell-derived Schwann cells, or bone marrow stromal cell-derived skeletal muscle cells; and a biocompatible polymer.

Belonging to the technical field of medical biomaterials, the invention in particular relates to a polylactic acid-chitosan composite nerve conduit. The composite nerve conduit is composed of oriented polylactic acid electrospun fiber and a chitosan layer wrapping the fiber. The invention also provides a preparation method of the polylactic acid / chitosan composite nerve conduit, and application of the nerve conduit in preparation of peripheral nerve defect repair materials. The polylactic acid-chitosan composite nerve conduit prepared by the invention has excellent biomechanical properties and superior hydrophilcity, also has good biological tissue compatibility, can significantly promote the attachment, migration and proliferation of Schwann cells, is easy to cut and preserve, and is simple to operate during clinical use, thus having good clinical application prospects in the peripheral nerve damage repair field.

The invention discloses a preparation method of a bidirectional gradient vascular endothelial growth factor (VEGF) by virtue of a nerve conduit. The preparation method comprises the following steps: preparing an electrostatic spinning polycaprolactone (PCL) conduit, carrying out bidirectional gradient ammonolysis on the PCL conduit, preparing a bidirectional gradient heparinization conduit by carrying out heparin coupling reaction, and preparing a bidirectional gradient VEGF conduit. According to the invention, a PCL conduit bidirectional ammonolysis device obtains a gradient conduit with high -NH2 density of a central part and the -NH2 density gradually reduced toward two ends; the bidirectional gradient VEGF functionalized nerve conduit provided by the invention is characterized in that quantity of the VEGFs loaded at the central part is high, and the quantity of the VEGFs loaded at the two ends is relatively low; endothelial cells at the two ends of an injured nerve can be enabled to migrate to a high concentration place at the center of the conduit by virtue of chemotaxis, so that directional vascularization of the nerve conduit from the two ends to the central part is accelerated; and a generated blood vessel can serve as a support for guiding Schwann cells to quickly migrate into the nerve conduit, so that regeneration of a nerve is promoted.

The present invention provides a method of inducing myelination of isolated motoneurons by preparing a non-biological substrate having thereon a covalently attached monolayer of DETA; depositing isolated motoneurons on the substrate in a defined serum-free medium; plating isolated Schwann cells cultured in the defined serum-free medium onto the motoneurons, thereby initiating a co-culture; and passaging the co-culture as necessary into fresh, defined serum-free medium supplemented with L-ascorbic acid at least until the motoneurons form Nodes of Ranvier indicative of myelination. The invention also includes a method of testing for new drugs effective in demyelinating diseases. Additionally, cellular products provided by the invention include an isolated motoneurons myelinated or remyelinated in vitro according to the methods disclosed.

The invention belongs to the field of traditional Chinese medicaments and relates to a compound preparation for promoting neuromuscular regeneration and a preparation method thereof. The compound preparation is prepared from raw material medicaments, such as codonopsis pilosula, astragalus, root of red-rooted salvia and the like, and medicinally acceptable auxiliary materials or auxiliary ingredients according to a specific weight ratio by a water extraction method. The result of a pharmacological test shows that the compound preparation can increase GAP-43 expression in early damaged nerve, can be competitively combined to a G protein reaction site, can promote quick growth of axon and can contribute to reconstruction of synapse. The result of an animal test shows that the compound preparation promotes metabolism of an organism, increases blood supply of a damaged part, eliminates degenerated myelin sheath, promotes fission and proliferation of Schwann cells and has obvious effect of accelerating regeneration of peripheral nerves after the peripheral nerves are damaged. The compound preparation can be further prepared into a medicament clinically used for promoting neuromuscular regeneration so as to bring benefit to more patients suffered from nerve injury.

Neurosupportive materials that possess strong tissue adhesion were synthesized by photocrosslinking two polymers, gelatin methacryloyl (GelMA) and methacryloyl-substituted tropoelastin (MeTro). The engineered materials exhibited tunable mechanical properties by varying the GelMA / MeTro ratio. In addition, GelMA / MeTro hydrogels exhibited 15-fold higher adhesive strength to nerve tissue ex vivo compared to traditionally used fibrin-based materials. Furthermore, the composites were shown to support Schwann cell (SC) viability and proliferation, as well as neurite extension and glial cell participation in vitro, which are essential cellular components for nerve regeneration. Finally, subcutaneously implanted GelMA / MeTro hydrogels exhibited slower degradation in vivo compared with pure GelMA, indicating its potential to support the growth of slowly regenerating nerves. Thus, GelMA / MeTro composites may be used as clinically relevant biomaterials to regenerate nerves and reduce the need for microsurgical suturing during nerve reconstruction.

A polymerizable unit that yields an electrochemically responsive polymer (advantageously pyrrole) is anchored by polymerization within a polycaprolactone matrix to form an electroactive scaffold upon which cells can be cultured and in which the micro- and nano-topological features of the polycaprolactone matrix are preserved. A scaffold manufactured in accordance with the preferred embodiment can support Schwann cells, which produce nerve growth factor when electrically stimulated. Nerve growth factor has been demonstrated to promote the regeneration of nerve tissue. By implanting the scaffold on which Schwann cells have been cultured into damaged nerve tissue and applying a voltage across the scaffold, nerve growth factor is produced, thereby promoting repair of the damaged nerve tissue.

The invention discloses a human NF2- / - vestibular schwannoma Schwann cell line and an establishment method thereof. The establishment method includes: A, separating Schwan cells in tumors to obtain human NF2- / - primary Schwann cells (human NF2- / - Schwann cells), and carrying out primary culture; B, establishing immunodeficient mouse sciatic nerve human NF2- / - primary Schwann cell transplanted tumor model; C, collecting transplanted tumors greater than 1 cm in diameter from mouse sciatic nerves by screening; D, separating Schwann cells, and carrying out primary cell culture; E, carrying out five successive generations and more of passage culture; F, performing screening to obtain NF2- / - vestibular Schwann cells which are consistent to human NF2- / - primary Schwann cells and transplanted tumor primary cell strains in cellular form and main biological characteristic and which are capable of infinite passage. The NF2- / - vestibular schwannoma Schwann cell line herein has the success rate of95% and above to grow transplanted tumors.

The invention discloses a nerve conduit of a magnesium wire and silk compositely woven structure and a preparation method of the nerve conduit. Medical metal magnesium wires with the high mechanical properties are adopted as axial yarn to be added into a silk woven structure, the longitudinal guiding effect is achieved, the radial compressive property of a woven skeleton can be further improved, meanwhile, the magnesium wires can be degraded slowly, growing and proliferating of Schwann cells are promoted, and injured nerves are recovered advantageously; and weaving yarn is degummed silk and has good biocompatibility, and a biological microenvironment for promoting nerve regeneration is formed while the good mechanical properties are ensured.

The invention belongs to the field of cell biology, and relates to a method for isolating and purifying schwann cells from adipose tissue, and an application thereof. The method comprises the following steps: (1) under the aseptic condition, peeling the adipose tissue, then cutting the adipose tissue into pieces and cleaning; (2) digesting the adipose tissue pieces through a digestive enzyme solution whose volume is 3-20 times of the volume of the adipose tissue pieces, wherein the digestive enzyme solution is ferment enzyme, collagenase NB4 or a mixed liquor consisting of the ferment enzyme and the collagenase NB4; (3) repeated beating upon the resulting mixed suspension for 3-5 minutes through a pipette; (4) processing the dissociated cells through centrifuging to obtain a cell aggregate, wherein relative centrifugal force is 600-1500g, centrifuging time is 3-5 minutes; (5) resuspending the cells from the step (4) through a culture medium for schwann cells, and inoculating the resuspended cells on a culture dish coated with fibronectin. According to the present invention, purity of the schwann cells can approach 95%. With the method provided by present invention, a plurality of high-quality schwann cells with high purity can be obtained with low-cost and high-efficiency.

The invention discloses a purpose of curcumin or a drug-loading system thereof in preparation of the drug for treating penis erection dysfunction. An experiment proves that the curcumin and a curcuminslow release drug film can up-regulate the expression of GAP-43 and MAP-2 to accelerate growth of the nerve axon and can up-regulate the expression of Oct-6 and Krox-20 to accelerate Schwann cells tosynthesize and secrete myelin sheath-associated protein so as to accelerate formation of the nerve axon myelin sheath. Meanwhile, repair and regeneration after a rat suffers from cavernous body nerveinjury, neural pathway continuity of the cavernous body is recovered, and expression of penis tissue nerve terminal nNOS mRNA and protein is increased. The penis erection function of the rat who suffers from the cavernous body nerve injury can be further improved, meanwhile, a penis tissue fibrosis degree is lowered, and rehabilitation of the penis after the cavernous body nerve injury is effectively accelerated. Therefore, the curcumin and the drug-loading system thereof perform a good application prospect in the preparation of the drug for treating the penis erection dysfunction and repairing the cavernous body nerve injury.

The invention provides an application of a miR containing a 5'- terminal specific seed base sequence, an improved Schwann cell and applications thereof, and relates to the technical field of central nervous system damage repairing. The miR is used to promote the compatibility and integration between Schwann cells and astrocyte; and the 5'- terminal specific seed base sequence is UAAGGCAC or AAGGCAC. The results of experiments show that after the expression level of matured miR containing a 5'- terminal specific seed base sequence is artificially improved, the expression of astrocyte related gene in improved Schwann cells is obviously reduced, the expression of neurotrophic factor genes (NT-3 and BDNF) is obviously increased, and improved Schwann cells are compatible with and fused with astrocyte. The improved Schwann cells can inhibit astrocyte, the integration of Schwann cells and astrocyte is promoted, and the formation of glial scars caused by astrocyte is inhibited.

A method for producing an engineered tissue scaffold for neural repair is described. The method includes tethering a hydrogel matrix seeded with tension-generating cells to a frame, and allowing the tension-generating cells to generate tension within the matrix, such that the cells self-align. The matrix may then be at least partially dehydrated to form a sheet. The tension-generating cells are stem cells capable of differentiating into cells having Schwann-cell-like properties, or are derived from such stem cells. In preferred embodiments, the cells are neural stem cells, for example conditionally immortalized neural stem cells of fetal cortex origin.

The invention relates to the technical field of cell biology, and in particular to a naked mole rate Schwann cell separation & purification and culture method. According to the method provided by the invention, Schwann cells are separated & purified and cultured from marrow DRG (dorsal root ganglion) of a fetal naked mole rate with the comprehensive use of a plurality of mediums, so that a reasonable method suitable for culturing the Schwann cells of a heterothermic mammal of rodent species, namely the naked mole rate, is found out. With the application of the method provided by the invention, a great amount of naked mole rate Schwann cells, which are normal in functions and activity, are conveniently, efficiently and economically obtained; the cells, when cultured under a hypoxia condition, can still reserve biological characteristics under an in-vivo state in an in-vitro environment, so as to facilitate further research, which is directly conducted in a pure in-vitro cell culture model, on special physiological functions of the naked mole rate Schwann cells; therefore, important theoretical bases are provided for researching a biological mechanism therein and for application to related clinical fields.

The invention discloses an application of circular RNA circ-Ankib1 in the preparation of a medicament for promoting nerve regeneration and repairing nerve injury. The result shows that the down-regulation or inhibition of the expression of circ-Ankib1 can promote the proliferation of Schwann cells, and is beneficial to the repair of peripheral nerve injury.

The invention discloses application of a java brucea fruit fat-soluble extract in preparation of a drug for promoting peripheral nerve regeneration. The java brucea fruit fat-soluble extract is obtained by extracting with a mixed solution of absolute ethyl alcohol and ethyl acetate. The research finds that the java brucea fruit fat-soluble extract can promote the growth of peripheral DRG neuronalneurons and promote the division and proliferation of peripheral nervous system glial cells Schwann cells; and the java brucea fruit fat-soluble extract has the effect of promoting peripheral nerve regeneration.

The invention belongs to the field of cell biology and relates to a method for separating and purifying schwann cells. The method sequentially comprises the following steps of: firstly, taking a nerve segment under the aseptic condition and removing an epineurium; secondly, digesting a nerve tract by using a NB4 composite collagenase and dispase mixed digestive enzyme solution and discarding the solution; thirdly, placing the mixture in a trypsin and an ethylene diamine tetraacetic acid (EDTA) for 5 to 10 minutes to obtain a nerve fiber from which a perineurium is removed; fourthly, placing the obtained nerve fiber in the mixed enzyme solution, and oscillating the mixture to obtain a cell suspension; and finally, performing centrifugation and heavy suspension. By using the method, a greatquantity of high-purity schwann cells are obtained in a low-cost and high-efficiency mode so that the abundant and high-quality schwann cells are supplied for repairing the damaged nerve.

The invention belongs to the field of biomedical engineering, and relates to a tissue engineered artificial optic nerve conduit and a preparation method thereof. The tissue engineered artificial optic nerve conduit comprises a hollow conduit woven from polylactic-polyglycolic acid filaments (PLGA), polyglycolic acid (PGA) filaments and transgenic schwann cell suspension, wherein chitosan polymer is coated on the surface of the conduit; an external bracket of artificial optic nerve is constructed by the PLGA hollow conduit; and the PGA filaments are filled in an internal bracket in which the artificial optic nerve is constructed. The conduit can effectively promote regeneration of damaged optic nerve axon, and has the advantages of persistently providing nerve nutritional support, guiding the regenerated nerve axon and the like; the conduit has high tissue compatibility and safety; the specifications of the transfected gene and the conduit can be adjusted according to requirements; and the conduit and the method can be used for experimental research of optic nerve regeneration and expected to promote regeneration and restoration of damaged optic nerve in clinical use.