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Polylactic acid-chitosan composite nerve conduit and preparation method thereof

A nerve conduit and chitosan technology, which is applied in the field of medical biomaterials, can solve the problems of regeneration and repair of unfavorable axon damage, affecting regeneration and repair of defective nerves, and low mechanical strength of nerve conduits, so as to avoid immune rejection and infectious diseases, The effect of repairing peripheral nerve defects and easy control of product quality

Inactive Publication Date: 2014-04-30
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the high porosity and specific surface area of ​​the electrospun fiber membrane, the texture of the fiber membrane is relatively soft, and the mechanical strength of the nerve guide obtained by rolling or directly collecting is low, and it is almost not resistant to external compressive loads. Afterwards, it is easy to collapse, which leads to the difficulty of axonal epitaxial growth.
After the pure polylactic acid (PLA) nerve conduit prepared by Ramakrishna et al. was implanted into the sciatic nerve defect in rats, it was found that 50% of the conduit collapsed, which seriously affected the regeneration and repair of the defective nerve (Ramakrishna et.al., In vivo study of novel nanofibrous intra-luminal guidance channels to promote nerve regeneration, J. Neural Eng., 2010, 7, 046003)
In addition, the polyester polymer fiber membrane has poor hydrophilicity, with a maximum of 138.2° reported in the literature, resulting in poor cell affinity, and it is difficult for cells to attach and proliferate on its surface, which is not conducive to the regeneration of axonal damage. repair

Method used

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  • Polylactic acid-chitosan composite nerve conduit and preparation method thereof
  • Polylactic acid-chitosan composite nerve conduit and preparation method thereof
  • Polylactic acid-chitosan composite nerve conduit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: Preparation of polylactic acid / chitosan composite nerve guide

[0059] (1) Dissolve 0.8 g of polylactic acid in 10 ml of hexafluoroisopropanol and stir overnight at room temperature to form a uniform and stable solution.

[0060] (2) Put the polylactic acid solution into the syringe, and connect the needle of the syringe to a high-voltage power supply. The solution supply flow is controlled at 5ml / h by a microflow pump, the applied voltage is 15kv, the distance between the high voltage end and the ground end is 15cm, the diameter of the roller used is 8cm, and the rotation speed is 3000rpm. Through this process, a polylactic acid nanofiber membrane with a diameter of about 630 nm and a thickness of about 0.2 mm can be collected.

[0061] (3) Immerse the polylactic acid nanofibrous membrane in dilute acetic acid solutions of chitosan with a concentration of 0.2%, 0.4% and 0.6% respectively (the number average molecular weight of chitosan is 500,000, the de...

Embodiment 2

[0064] Embodiment 2: Observation of microscopic morphology of polylactic acid / chitosan composite nerve conduit

[0065] The surface and cross-sectional morphology of polylactic acid / chitosan composite nerve conduits with different chitosan compounding amounts were analyzed by field emission scanning electron microscope (JSM-6700F, JEOL, Japan), and the accelerating voltage was 10kV.

[0066] The result is as figure 2 As shown, it can be seen that pure polylactic acid ( figure 2 a) and polylactic acid / chitosan composite fiber ( figure 2 b, c, d) are all highly oriented and arranged; there is no chitosan wrapping on the surface of pure polylactic acid fiber, the pore size is large, and the porosity is high; with the increase of chitosan compounding amount, chitosan wrapping can be clearly observed On the surface of PLA fibers and in the voids between fibers, the porosity decreased gradually. It can be seen from the cross-sectional morphology that the pure polylactic acid f...

Embodiment 3

[0067] Embodiment 3: Research on the biomechanical properties of polylactic acid / chitosan composite nerve guide

[0068] Cut the polylactic acid / chitosan composite fiber membrane into a sample with a length and width of 40×4mm, trim the two sides of the sample flat to prevent cracks, and then use a spiral micrometer to measure the thickness of each sample, and select different parts Measure three times and take the average value. Then, the tensile mechanical properties were measured by a universal mechanical testing machine, and the rising speed of the crosshead was 2mm / min. The polylactic acid / chitosan composite nerve guide is placed horizontally on the test bench, and the anti-pressure performance is measured by a universal mechanical testing machine. The descending speed of the crosshead is 0.5mm / min.

[0069] The results showed that the tensile strength of polylactic acid / chitosan composite fiber membrane was significantly higher than that of pure polylactic acid fiber, a...

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Abstract

Belonging to the technical field of medical biomaterials, the invention in particular relates to a polylactic acid-chitosan composite nerve conduit. The composite nerve conduit is composed of oriented polylactic acid electrospun fiber and a chitosan layer wrapping the fiber. The invention also provides a preparation method of the polylactic acid / chitosan composite nerve conduit, and application of the nerve conduit in preparation of peripheral nerve defect repair materials. The polylactic acid-chitosan composite nerve conduit prepared by the invention has excellent biomechanical properties and superior hydrophilcity, also has good biological tissue compatibility, can significantly promote the attachment, migration and proliferation of Schwann cells, is easy to cut and preserve, and is simple to operate during clinical use, thus having good clinical application prospects in the peripheral nerve damage repair field.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a polylactic acid / chitosan composite nerve catheter and its preparation method and application. The catheter material has good biomechanical strength, hydrophilicity and the effect of promoting Schwann cell proliferation , can be used for regeneration and repair of peripheral nerve defects. Background technique [0002] Peripheral nerve injury is a common severe trauma to the extremities in clinical practice, and it is more common in peacetime and wartime. According to the "Science Times" report, among the annual new trauma cases in the world, peripheral nerve injury cases account for about 1.5% to 4.0%. There are 600,000 to 900,000 new cases of peripheral nerve injury in my country every year, of which about 300,000 to 450,000 cases require nerve transplantation to repair nerves. The repair of peripheral nerve defects, especially the repair of long-dis...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/20D06M15/03D06M101/32
Inventor 侯春林窦源东林浩东魏长征
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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