Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for inducing differentiation from human umbilical cord mesenchymai stem cells (hucMSCs) into neural cells

A technology of stem cells and nerve cells, applied in the field of cell biology

Inactive Publication Date: 2011-09-21
SHANGHAI FIRST PEOPLES HOSPITAL
View PDF2 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the induction of HUCMSCs into neuron-like cells by chemical or co-culture methods and their application in the research of nerve injury repair

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inducing differentiation from human umbilical cord mesenchymai stem cells (hucMSCs) into neural cells
  • Method for inducing differentiation from human umbilical cord mesenchymai stem cells (hucMSCs) into neural cells
  • Method for inducing differentiation from human umbilical cord mesenchymai stem cells (hucMSCs) into neural cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] Experimental steps:

[0027] 1. Materials and methods

[0028] The umbilical cords were obtained from full-term caesarean section infants in the Department of Obstetrics and Gynecology, Shanghai First People's Hospital, with the authorization and consent of their parents.

[0029] 2. Main reagents and factors

[0030] Ten 2-week-old C57BL / 6 mice (Shanghai Experimental Animal Breeding Center, Chinese Academy of Sciences), DMEM medium (Gibco, USA), complex collagenase NB4 (Serva, Germany), fetal bovine serum (FBS: Hyclone, Australia), phosphoric acid Salt buffer (PBS, PH7.2), Schwann cell culture medium (SCCM, Schwann cells culture medium containing 10ngheregulin-β-1, 2uM forsklin, 10% FBS, 100u / ml penicillin, 100u / ml streptomycin and high sugar DMEM). Rabbit anti-human NF-200, B-tubulin III, GFAP, Nestin (Milipore, USA) flow cytometry antibodies were purchased from (BD, USA).

[0031] 3. Isolation of umbilical cord mesenchymal cells

[0032] Remove the umbilical cor...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of cell biology and relates to a method for inducing differentiation from human umbilical cord mesenchymai stem cells (hucMSCs) into neural cells. The method comprises that HUCMSCs and Schwann cells are putted in Transwell with apertures of 0.4 microns to be co-cultured separately; a Schwann cell culture medium is utilized as a cell culture medium and half or all of the cell culture medium is replaced every three days; and then neural cells can be obtained after two weeks of culturing. Through a separate co-culturing method utilized by the invention, an umbilical cord can be digested by a mixture of composite collagenase NB4 and hyaluronidase and thus a large amount of HUCMSCs can be separated. High purity HUCMSCs can be obtained in third generation passage cells. In the invention, HUCMSCs are induced and differentiate into neural cells, wherein a cell differentiation rate is over 70%. Expressing rate ranges of specific markers, such as NF-200, nestin, Betar-III-tubulin, etc., of neural cells are from 70 to 80%.

Description

technical field [0001] The invention belongs to the field of cell biology and relates to a method for stably inducing human umbilical cord mesenchymal stem cells. Background technique [0002] The repair of nervous system trauma is still a big problem to be solved in clinical practice. Autologous nerve transplantation, which is the gold standard for repairing nerve defects, has the disadvantages of limited sources and left sensory impairment at the donor site. [0003] Stem cells have the characteristics of abundant sources, easy isolation and culture, strong in vitro proliferation ability, and multi-directional differentiation potential. In recent years, they have become a hot spot in the research of seed cells for tissue engineering products. Studies have found that embryonic stem cells (ESCs), neural stem cells (NSCs), bone marrow stromal stem cells (BMSCs), and umbilical cord blood stem cells all have the potential to differentiate into nerve cells, and can improve thei...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 沈尊理祝加学秦金保
Owner SHANGHAI FIRST PEOPLES HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com