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A method for superovulation of naked mole rats

A technique for superovulation and naked mole rats, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, peptide/protein ingredients, etc., and can solve the problem that rats or mice are not suitable for naked mole rats.

Active Publication Date: 2020-08-11
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In addition, naked mole rats have special physiological characteristics, and the existing superovulation procedures for rats or mice are not suitable for naked mole rats

Method used

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  • A method for superovulation of naked mole rats
  • A method for superovulation of naked mole rats

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Embodiment 1

[0020] Example 1 Superovulation in naked mole rats

[0021] 1. Pharmacy:

[0022] Pregnant horse serum (Pregnant Mare Serum Gonadotropin, PMSG): Ningbo No. 2 Hormone Factory, China;

[0023] Human chorionic gonadotrophin (HCG): Ningbo Sunshine Pharmaceutical Co., Ltd., China.

[0024] 2. Selection of age-appropriate healthy animals

[0025] In the best breeding season of naked mole-rats (March-June each year), 40 healthy naked mole-rats aged 18-30 months, weighing 50±0.2g, were individually reared during the treatment period. Because naked mole-rats are a seasonally-breeding species that degenerates overall gonadal function during the non-breeding season and do not respond to superovulation-related drugs, this work must be carried out during the breeding season. Naked mole rats complete sexual maturity at the age of 8 months, and complete physical maturity at the age of 14 months. The best breeding period is between 18 and 30 months. Judging from the body condition, the bod...

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Abstract

The invention relates to the field of experimental animal production, and in particular relates to a superovulation method of naked mole rats. The superovulation method comprises the following steps of: at the 1st, 3th, 5th and 7th days after the oestrus is ended, injecting PMSG (Pregnant Mare Serum Gonadotropin) into the abdominal cavities of the female naked mole rats; at the 7th day, injecting HCG with the dosage of 0.12IU / g (body weight) into the abdominal cavities of the naked mole rats; at the time of 1 hour after injection of HCG, starting to cage the female naked mole rats and male rats with fertility for free mating. The superovulation method determines the implementation schedule, the drug ingredients and dosage by verification of numerous tests. The superovulation method has the advantages that a set of superovulation program for the naked mole rats is made, so that the blank that the superovulation program is not made temporarily after introduction of the naked mole rats in China is filled; every experimental animal responses to the superovulation and can form 51-55 follicles. The adopted drugs and program are more suitable for the naked mole rats, and the superovulation program is more economical.

Description

technical field [0001] The invention relates to the technical field of experimental animal production, in particular to a superovulation method for naked mole rats. Background technique [0002] Superovulation is the use of exogenous hormones to induce the simultaneous development of multiple follicles in the ovary during a period of time during the estrus cycle of female animals, reducing the follicle development cycle and increasing the number of mature follicles to achieve the effect of increasing the reproductive rate. At present, the superovulation technology of rats and mice is very mature, and has corresponding operating standards, and has been applied on a large scale in the purification of rats and mice and the production of genetically modified animals. [0003] The naked mole rat is an underground animal native to eastern Africa, including areas such as Kenya, Ethiopia and Somalia, where it lives a social life. There are usually 40-90 individuals in a naked mole ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D19/00A61D7/00A61K38/24A61P15/08
CPCA61D7/00A61D19/00A61K38/24
Inventor 崔淑芳杨文静徐晨李周桐刘攀蔡丽萍林丽芳程继帅
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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