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Superovulation method of naked mole rats

A technology of superovulation and naked mole rats, which can be applied to medical formulas, medical preparations containing active ingredients, veterinary instruments, etc., and can solve the problems that rats or mice are not suitable for naked mole rats

Active Publication Date: 2017-01-18
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In addition, naked mole rats have special physiological characteristics, and the existing superovulation procedures for rats or mice are not suitable for naked mole rats

Method used

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  • Superovulation method of naked mole rats
  • Superovulation method of naked mole rats

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Embodiment 1

[0020] Embodiment 1 naked mole rat superovulation

[0021] 1. Drugs:

[0022] Pregnant Mare Serum Gonadotropin (PMSG): China Ningbo Second Hormone Factory;

[0023] Human chorionic gonadotropin (HCG): Ningbo Sansheng Pharmaceutical Co., Ltd., China.

[0024] 2. Selection of age-appropriate healthy animals

[0025] In the best breeding season of naked mole rats (March-June every year), 40 healthy naked mole rats aged 18-30 months were selected, with a weight of 50±0.2g, and they were kept alone during the treatment period. Because naked mole rats are a species that reproduces seasonally, their overall gonadal function degenerates during the non-breeding season, and they do not respond to superovulation-related drugs, so this work must be carried out during the breeding season. Naked mole rats reach sexual maturity at 8 months, body maturity at 14 months, and 18-30 months is the best breeding period. From the perspective of body condition, the body weight of 45-55g is moderat...

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Abstract

The invention relates to the field of experimental animal production, and in particular relates to a superovulation method of naked mole rats. The superovulation method comprises the following steps of: at the 1st, 3th, 5th and 7th days after the oestrus is ended, injecting PMSG (Pregnant Mare Serum Gonadotropin) into the abdominal cavities of the female naked mole rats; at the 7th day, injecting HCG with the dosage of 0.12IU / g (body weight) into the abdominal cavities of the naked mole rats; at the time of 1 hour after injection of HCG, starting to cage the female naked mole rats and male rats with fertility for free mating. The superovulation method determines the implementation schedule, the drug ingredients and dosage by verification of numerous tests. The superovulation method has the advantages that a set of superovulation program for the naked mole rats is made, so that the blank that the superovulation program is not made temporarily after introduction of the naked mole rats in China is filled; every experimental animal responses to the superovulation and can form 51-55 follicles. The adopted drugs and program are more suitable for the naked mole rats, and the superovulation program is more economical.

Description

technical field [0001] The invention relates to the technical field of experimental animal production, in particular to a method for superovulation of naked mole rats. Background technique [0002] Superovulation is to use exogenous hormones to induce the simultaneous development of multiple follicles in the ovary of female animals during a period of estrus cycle, reduce the follicle development cycle, increase the number of mature follicles, and achieve the effect of increasing the reproductive rate. At present, the superexclusion technology of rats and mice is very mature, and has corresponding operating standards, and has been widely used in the purification of rats and mice and the production of genetically modified animals. [0003] The naked mole rat is a social animal native to the subterranean regions of eastern Africa, including Kenya, Ethiopia and Somalia. There are generally 40-90 individuals in a naked mole rat colony, and the maximum can reach more than 300. W...

Claims

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Application Information

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IPC IPC(8): A61D19/00A61D7/00A61K38/24A61P15/08
CPCA61D7/00A61D19/00A61K38/24
Inventor 崔淑芳杨文静徐晨李周桐刘攀蔡丽萍林丽芳程继帅
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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