35results about How to "Improve functional status" patented technology

The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat astroglia cells. The astroglia cells are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat astroglia cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat astroglia cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat astroglia cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.

Salivary gland cells and adipose-derived stem cells are respectively cultured by adopting a digestion method; co-culture is performed on the salivary gland cells and the adipose-derived stem cells with a transwell culture chamber and a 24-well culture plate; the salivary gland cells are inoculated in an inner chamber, and the adipose-derived stem cells are inoculated in an outer chamber; a DMEM (dulbecco's modified eagle medium) containing fetal calf serum of which the volume fraction is 10 percent is adopted as the culture medium; platelet-rich fibrin leach liquor of which the mass fraction is 0.5 to 5 percent is added to induce the transdifferentiation of the adipose-derived stem cells into the salivary gland acinus-like cells. According to a method disclosed by the invention, the efficiency of the transdifferentiation of the adipose-derived stem cells into the salivary gland acinus-like cells can be effectively improved, and seed cells and a new transdifferentiation method are provided for the tissue engineering technology, regenerative medicine research and application.

The invention discloses ointment curing newborn red buttock and a preparing method of the ointment. The ointment comprises the following components by weight: 100 to 250 parts of arnebia root, 15 to 200 parts of fish liver oil, 30 to 150 parts of tannic acid, 20 to 200 parts of glycerol, and 900 to 1100 parts of vaseline. The method comprises the following steps: taking arnebia root according to the proportion of the recipe; extracting effective components of arnebia root through fat extraction and water extraction; adding tannic acid, glycerol, fish liver oil and vaseline according to the proportion of the recipe into the effective components; stirring uniformly; and obtaining ointment curing newborn red buttock through an emulsion homogenization mechanism. The ointment is simple in recipe, good in curative effect, low in price and convenient for use, and has a better curing effect for skin mucosal diseases such as anal fissure, scalding and bedsore.

The invention discloses a traditional-Chinese-medicine compound prescription for reducing the relapse rate of tumor patients and relates to the traditional-Chinese-medicine compound prescription. The traditional-Chinese-medicine compound prescription contains the following components in mass by weight: 10-50g of radix salviae miltiorrhizae, 10-50g of curcuma zedoary, 3-20g of radix pseudostellariae, 6-30g of poria cocos, 3-30g of pinellia ternata, 3-30g of Ussuriensis Fritillary Bulb, 3-50g of oyster, 3-50g of lapis chloriti, 3-15g of radix aucklandiae, 3-30g of fried rice sprout and 3-30g of tuber fleeceflower stem. Proved by animal experiments, the traditional-Chinese-medicine compound prescription has the effects of obviously prolonging the life period with cancer, reducing antagonistic leukocytes, promoting cancer cell apoptosis, inhibiting proliferation of tumor histopathology and the like for mice with chemotherapy for the solid transplanted tumor and the ascitic tumor of the liver cancer; for all the patients after indications and operation chemotherapy and the patients after pure chemotherapy, the purposes of prolonging the life period, improving the living quality and reducing the relapse rate can be achieved; the traditional-Chinese-medicine compound prescription is developed completely based on health-care products with China health food approval number of Chinese herbal medicines for health-care foods; and as a long-term target, various dosage forms of Chinese patent medicines with more obvious effects are developed.