Primary separation culture method for bone marrow mesenchymal stem cells of naked mole rats

Abstract

The invention relates to the field of stem cell culture, and particularly discloses a primary separation culture method for bone marrow mesenchymal stem cells of naked mole rats. The primary separation culture method includes steps of 1), washing bone marrow of the naked mole rats; 2), sieving bone marrow washed substances by the aid of cell strainers; 3), breaking red blood cells; 4), carrying out direct plate laying. The primary separation culture method for the bone marrow mesenchymal stem cells of the naked mole rats has the advantages that the primary separation culture method is established for the bone marrow mesenchymal stem cells of the naked mole rats, biological characteristics of the bone marrow mesenchymal stem cells cultured by the aid of the primary separation culture method are evaluated, the fact that the bone marrow mesenchymal stem cells with extremely high purity and differentiation capacity can be obtained by the aid of the primary separation culture method is proved, and accordingly intensive study on characteristics of stem cells of the naked mole rats and exploration on characteristics of somatic cells, which are difficult to separate but can be obtained from the stem cells by means of transformation, of the naked mole rats can be possibly carried out.

Description

technical field [0001] The invention relates to the field of stem cell culture, in particular to a method for primary isolation and culture of naked mole rat bone marrow mesenchymal stem cells. Background technique [0002] The naked mole rat is a digging rodent distributed in parts of East Africa, and belongs to the class Mammalia and Rodentia in taxonomy. Naked mole rats live their entire lives in the dark, underground, adapt to low-oxygen environments, and have the longest lifespan among rodents; they are super immune to cancer. Longevity, anti-aging, hypoxia resistance, anti-tumor and other properties of naked mole rats have attracted widespread attention from scientists, and research results on these properties have been published in famous journals such as Nature and PNAS. [0003] Bone marrow mesenchymal stem cells (BMSCs) are adult stem cells isolated from bone marrow with high self-renewal ability and multilineage differentiation potential. Although mesenchymal st...

AI Technical Summary

Problems solved by technology

The cost of flow cytometry and immunomagnetic bead separation is high, and the scope of promotion is limited; the cell viability obtained by density gradient centrifugation is low when isolating bone marrow mesenchymal stem cells of small animals; the cell viability obtained by direct attachment method is high , but not suitable for animals with excess erythrocytosis due to erythrocytes affecting the attachment of target cells

Naked mole rats are close to mice in size, adapt to low oxygen environment, and have high red blood cell content. The current culture method is not conducive to obtaining high activity and quantity of naked mole rat bone marrow mesenchymal stem cells

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