30results about How to "Controllable density" patented technology

The invention provides a method for large-scale preparation of micro-organism microcapsules, belonging to the technical field of micro-organism and biology. The method of the invention comprises the steps as follows: (1) capsule solution preparation: preparing the sodium alginate solution with the mass volume specific concentration of 1-2% w / v and carrying out the operation of sterilization; (2) preparation of curing agent: preparing the CaCl2 solution of 0.1-0.3mol / L and sterilizing; (3) preparation of the micro-organism microcapsules: micro-organism seed liquid with the logarithmic growth phase is added into the sterilization capsule solution till that the density of the solution is about 10<6>cfu / ml; the solution is then injected into the aseptic CaCl2 solution through the aseptic filter film and a nozzle by high-pressure air, then mixed and solidified till that supernatant is precipitated so as to gain the microcapsule and to wash the capsule; (4) after-covering culture: the microcapsules covered by micro-organism cells are arranged in fermentation broth and cultured till logarithm growth telophase, so as to gain the micro-organism microcapsule by filtration and separation. The method of the invention has the advantages of overcoming the defect that the micro-organism microcapsule which is prepared by covering-method before the sterilization can not be produced in large-scale, large production, simple operation and low cost of material and equipments.

The invention discloses a solvent-resistant composite nanofiltration membrane and a preparation method thereof, belonging to the field of organic composite materials. The solvent-resistant composite nanofiltration membrane is composed of short chain segments of poly-p-phenylene terephthalamide and polyaryl ether sulfone with amino branched structure. The preparation method comprises three steps of dissolving poly-p-phenylene terephthalamide nanofibers, synthesizing polyaryl ether sulfone with amino branched structure and preparing solvent-resistant composite nanofiltration membrane. The membrane made by the invention has high flux transmission channel, good chemical stability and solvent resistance.

The invention discloses a method for preparing a coating microcapsule before microbial fermentation by utilizing a common fermentation tank. The method comprises steps of: preparation of a capsule material solution, preparation of an aqueous mixed solution, preparation of an oil mixed solution, preparation of a settling agent, preparation of microcapsules and culturing after coating, etc. By the method, microcapsules of which the diameter is 100-400 micrometers can be prepared, and the microcapsules have good sphericity degree and the yield of the product is higher than 95%. The innovation point of the method disclosed by the invention is that the method utilizes the general fermentation tank for preparation and fermentation of microcapsules without any modification, really realizes large-scale production of probiotic microcapsules, and has better product yield, sphericity degree and dispersibility than those of the existing industrial before-fermentation coating production method. The method disclosed by the invention is suitable for preparation of microorganism microcapsule in a plurality of fields such as fodders, foods and medicines, etc.

The present invention discloses micro-capsule preparation and a method for preparing the same, belonging to the microorganism field and the preparation field. The micro-capsule preparation uses sodium alginate as capsule material solution and CaCl2 as curing agent and contains Enterococcus Faecium the preservation number of which is CGMCC No.2516. The invention has the advantages that: the pre-embedding and after fermentation method is adopted, thereby thalli can be proliferated continuously under the micro-capsule microenvironment; the size of the cell density is controllable; the density of the micro-capsule thalli higher than that of the after embedding method can be reached; the pre-embedding method greatly reduces the utilization amount of cells during the encapsulization process, avoids the adverse factors of low encapsulization efficiency and productive rate due to impact of the cell density in the after embedding process, and more easily obtains encapsulated products with better shape and stability.