288 results about "Staphylococcus albus" patented technology

The present invention provides probe arrays and methods of using the same for concurrent and discriminable detection of multiple strains of different species. In one aspect, the probe arrays of the present invention are nucleic acid arrays comprising (1) a first group of probes, each of which is specific to a different respective strain of a first species; and (2) a second group of probes, each of which is specific to a different respective strain of a second species. In many embodiments, the nucleic acid arrays of the present invention further include a third group of probes, each of which is specific to a different strain of a third species. In one example, a nucleic acid array of the present invention includes probes for sequences selected from SEQ ID NOs: 1 to 18,598, and can discriminably detect different strains of Streptococcus pyogenes, Streptococcus agalactiae and Staphylococcus epidermidis.

The present invention relates to DNA-based methods for universal bacterial detection, for specific detection of the common bacterial pathogens Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Staphylococcus saprophyticus, Streptococcus pyogenes, Haemophilus influenzae and Moraxella catarrhalis as well as for specific detection of commonly encountered and clinically relevant bacterial antibiotic resistance genes directly from clinical specimens or, alternatively, from a bacterial colony. The above bacterial species can account for as much as 80% of bacterial pathogens isolated in routine microbiology laboratories. The core of this invention consists primarily of the DNA sequences from all species-specific genomic DNA fragments selected by hybridization from genomic libraries or, alternatively, selected from data banks as well as any oligonucleotide sequences derived from these sequences which can be used as probes or amplification primers for PCR or any other nucleic acid amplification methods. This invention also includes DNA sequences from the selected clinically relevant antibiotic resistance genes. With these methods, bacteria can be detected (universal primers and / or probes) and identified (species-specific primers and / or probes) directly from the clinical specimens or from an isolated bacterial colony. Bacteria are further evaluated for their putative susceptibility to antibiotics by resistance gene detection (antibiotic resistance gene specific primers and / or probes). Diagnostic kits for the detection of the presence, for the bacterial identification of the above-mentioned bacterial species and for the detection of antibiotic resistance genes are also claimed. These kits for the rapid (one hour or less) and accurate diagnosis of bacterial infections and antibiotic resistance will gradually replace conventional methods currently used in clinical microbiology laboratories for routine diagnosis. They should provide tools to clinicians to help prescribe promptly optimal treatments when necessary. Consequently, these tests should contribute to saving human lives, rationalizing treatment, reducing the development of antibiotic resistance and avoid unnecessary hospitalizations.

The subject matter of the present invention is a bacteria or mix of bacteria having an antagonistic activity with respect to stains of S. aureus, P. aeruginosa, Streptococcus pyogenes, Enterococcus faecium, Enterobacter cloacae, Proteus mirabilis, Bacteroides fragilis Staphylococcus epidermidis, Propionibacterium acnes, Candida albicans and / or Malassezia furfur as well as the use thereof in the treatment and / or prevention of infections or colonisations related to those pathogens. The invention pertains to care products containing one or more non-pathogenic antagonistic strains intended to prevent and / or treat infections or colonisations on skin, wounds, mucous membranes and appendages.

The invention relates to the technical field of fibers, in particular to a mite and mosquito-bite preventing underwear, sleepwear and home wear which is characterized by comprising the following processing steps of: preparing medical liquid; carrying out dyeing and printing and other pre-treatments on cottons or cotton blended fabrics; preparing bleaching and dyeing liquid; dyeing cloth; carrying out high temperature tentering; and processing so as to prepare the underwear, the sleepwear and the home wear. According to the invention, the fabrics provided by the invention have the obvious effects of mite preventing, mosquito preventing, anti-bacterium, deodorization, rot resistance, itching relieving, blood circulation invigoration and meridians activation and the like; the repellent rate of dust mite is as high as 99 percent; the repellent rate of mosquito is 95 percent; the repellent rate of termite is 95 percent; fabric-touching pathogens such as staphylococcus aureus, staphylococcus epidermidis, gonococcus and esherichia coli and the like can be completely killed; all efficacies of the fabrics still reach to over 97 percent after the fabrics are washed for 50 times; and the fabrics have the advantages of no irritation to skin, no anaphylactic reaction, no toxicity to the human body, comfort for wearing and can effectively prevent the infection of asthma and other diseases.

The invention belongs to the medical technical field and discloses the application of high-purity in the preparation of antibacterial medicine, antiviral medicine and other medicines. The invention proves the antipyretic, anti-inflammation, immunity enhancement, antibacterial and anti-virus effects of high-purity forsythiaside through the following experiments: (1) the experiment that high-purity forsythiaside has antipyretic effect on fever rats; (2) the experiment that high-purity forsythiaside inhibits xylene-induced mice ear edema, xylene-reduced capillary permeability of mice; (3) the experiment that high-purity forsythiaside increases carbon clearance ability of mice and increases the lymphocyte transformation rate; (4) the experiment that high-purity forsythiaside has vitro anti-influenza A type virus effect and in-vivo anti-respiratory syncytial virus effect; (5) the experiment that high-purity forsythiaside has vitro inhibition effects on beta-hemolytic streptococcus, staphylococcus aureus, streptococcus viridans and staphylococcus epidermidis.

Provided are: a skin-protecting agent and an external preparation for skin protection, which not only synergistically work in combination with a plant polysaccharide but also has an effect of promoting the production of glycerol derived from Staphylococcus epidermidis, which is a skin resident bacterium useful on the skin, and the production of antimicrobial peptides derived from skin epidermal keratinocytes and exhibits excellent effect of improving the touch feeling when applied to the skin. More specifically, the present invention provides a skin protective agent and an external preparationfor skin protection, which comprise a lactic acid bacterium belonging to the genus enterococcus.

The invention relates an air cleaner, solving the problem that the current air cleaners isn't disclosed the date of removing pneumococcus klebsiella and staphylococcus epidermidis and the date of effective degerming life, and the cleaning effect of current air cleaners is not good. The invention adopts the technical project that the silver particles below 100 nanometer are fixed on the surface of carrier to solve the problem.

The present invention is based on the surprising antiseptic nature of chlorhexidine when combined with essential oils. It has been found that chlorhexidine, combined with an essential oil, is surprisingly effective at penetrating the skin surface and providing an antiseptic effect within the skin. It has also been found that a combination of chlorhexidine and eucalyptus oil is surprisingly effective as an antimicrobial against Staphylococcus epidermidis.

The invention relates to the technical field of medical detection, and in particular, relates to a primer and probe combination and a kit for detecting human pathogenic bacteria. The kit comprises theprimer and probe combination for detecting pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, staphylococcus epidermidis, staphylococcus aureus, enterococcus faecium, human staphylococcus and acinetobacter baumannii. According to the kit, the primer and probe combination is adopted, multiple digital PCR is combined, the pathogenic bacteria types and the target nucleic acid copy number in the detection range can be rapidly and accurately identified, the detection method has high sensitivity, and positive detection of low-copy nucleic acid fragments of pathogenic bacteria is guaranteed; meanwhile, the variety of the pathogenic bacteria or the change of the copy number of the target nucleic acid fragment in the body of a pathogenic bacteria infected patient can be dynamically monitored, the treatment effect is assisted and evaluated in time, and reference is provided for optimization of a doctor clinical scheme.

The invention relates to a 3-carbonyl-6-ethoxycarbonyl-thiazole pyrimidine compound and a synthesis method and application thereof. The compound has a right structure formula. The 3-carbonyl-6-ethoxycarbonyl-thiazole pyrimidine compound serving as an inhibiting agent of YyCG histidine kinase protein for a staphylococcus epidermidis signal transduction system has a novel structure, and the adopted method for preparing the compound is simple and easy to implement. The test results of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) show that the compound has excellent inhibitory action on the formation of a staphylococcal biological film and can effectively kill and wound the bacteria in the staphylococcal biological film.

The invention provides a staphylococcus aureus phage strain VB_SavM_JYL01. The staphylococcus aureus phage stain VB_SavM_JYL01 has been preserved in the China Center for Type Culture Collection on 25th March, 2018, the preservation name is Staphylococcus aureus phage VB_SavM_JYL01, and the preservation number is CCTCC M 2018154. The staphylococcus aureus phage strain has a wide lysis spectrum, thus staphylococcus aureus can be lysed, certain staphylococcus epidermidis can also be lysed, host bacteria 206 can be lysed, and staphylococcus clinical strains separated from the environment or fecesof diseased animals can also be lysed; the staphylococcus aureus phage can be used independently or in combination with other substances, and a safe and nontoxic phage disinfecting and killing productis provided for disinfecting and purifying the environment.

The present invention relates to gene chip for detecting several kinds of common bacterial pathogens, and the gene chip includes solid carrier and oligonucleotide probe fixed on the carrier. The chip has up to 200 low density distributed sample points. The oligonucleotide detecting probe includes DNA' s selected from nucleotide sequences corresponding to the genomes of nine kinds of common bacterial pathogens, including Staphylococcus aureus, Staphylococcus epidermidis, bacillus pyocyaneus, colibacillus, etc. The said chip together with sample treating reagent, hybridizing reagent, color reagent and the specification constitutes the detection kit. The present invention has high detection efficiency.

The invention discloses a method for preparing a Chinese herbal medicine disinfectant for abating air pollution through an air purifier. The method comprises the steps: mixing all formula raw pharmaceutical materials such as 30g of folium isatidis, 16g of herba leonuri and the like, loading the mixed raw pharmaceutical material to an extracting tank, adding 600L of water into the extracting tank, carrying out heated extraction for 2 hours, obtaining distilled aromatic water from the top of the extracting tank, putting pharmaceutical filtrated residue into the extracting tank, and carrying out second-time extraction so as to obtain a filtrate and distilled aromatic water; and mixing the two kinds of fine medicine liquid, carrying out filtration, and then, concentrating the filtrate to be 600L, thereby obtaining the Chinese herbal medicine disinfectant. According to the Chinese herbal medicine disinfectant, the purification ratio of removing cigarette smoke, PM2.5, formaldehyde and ammonia gas from air reaches 91-99.9%, and the in-air and direct killing rate to influenza viruses, Legionnella, streptococcus pneumoniae and staphylococcus albus reaches 84-99.9%, so that the Chinese herbal medicine disinfectant has outstanding advantages.

The invention discloses preparation and application of recombinant plectasin. The method comprises designing plectasin gene according to preferred codons of Pichia pastoris, wherein possible nucleotide sequences of the plectasin gene are expressed in SEQ ID NO. 1, constructing recombinant expression vectors pPICPlectasin and recombinant genetic engineering bacteria Pichia pastoris X33pPICPlectasin (CGMCC NO. 3564), carrying out a high density fermentation process on the recombinant genetic engineering bacteria Pichia pastoris having a high expression level, wherein a total protein concentration of supernate from the high density fermentation process is 729 microgrammes per milliliter, dialyzing and freeze-drying the supernate, and orderly carrying out a gel filtration chromatography treatment and a reversed phase high performance liquid chromatogram treatment on the freeze-dried supernate to obtain high purity recombinant plectasin. The high purity recombinant plectasin is not hemolytic, has favorable PH stability, heat stability and anti-pepsin activity, and can inhibit effectively the growth of gram-positive pathogen Streptococcus pneumonia, staphylococcus aureus and staphylococcus epidermidis. Therefore, the high purity recombinant plectasin can be utilized for treating and preventing gram-positive bacterium and especially streptococcus and has potential antimicrobial drug development values.

The invention discloses antibacterial peptide, and belongs to the field of molecular biology. The antibacterial peptide has an amino acid sequence which is as shown in SEQ ID NO:2: Asn Trp Phe Ala Ile Thr Asn Trp Leu Trp Tyr Ile Lys Lys Lys Lys. The antibacterial peptide disclosed by the invention not only has an obvious inhibiting effect on gram negative bacteria, such as Escherichia coli, bacillus subtilis and staphylococcus epidermidis, and positive bacteria, but also has very low hemolytic activity and cytotoxicity, and the antibacterial peptide has good stability and high antibacterial activity, has an efficient broad-spectrum antibacterial effect and can be used as antibiotics substitutes.

The invention discloses a ceramic tile containing loess-doll and a preparation method thereof. The ceramic tile is prepared from the following raw materials in parts by weight: 55-65 parts of quartz powder, 30-40 parts of china clay, 15-25 parts of albite, 10-15 parts of zeolite, 16-28 parts of loess-doll, 11-22 parts of medical stones, 5-10 parts of sericite, 3-7 parts of nanometer silicon nitride, 20-30 parts of modified clay, 6-12 parts of boron mud, 2-6 parts of chaff powder and 4-9 parts of coarse whiting. The raw materials of loess-doll, medical stones and the like are added into the ceramic tile, so that substances of ammonia, hydrogen sulfide, organochlorine, cyanide and the like harm to human health can be adsorbed and decomposed, the growth of bacteria of staphylococcus albus, escherichelloa coli and the like and various kinds of fungi can also be inhibited, and indoor environment can be effectively improved and purified. The prepared ceramic tile has the characteristics of high strength, low water absorption rate, high abrasive resistance and weather ability and the like, and is durable in use.

The invention discloses an essential oil composition prepared by performing rectification and compounding processing on the raw materials comprising 4-terpilenol type tea-tree-oil and eucalyptus grandis x e.urophylla essential oil, and provides the formula, a preparation method and the composition content of the essential oil composition; and the composition has antibacterial insecticidal functions. A gas chromatography method is employed to determine the main content. The method is accurate, stable and easy to operate, is adaptable for industrial production demands, and can help to effectively control the quality of the essential oil composition. The essential oil composition has small toxicity, has different levels of inhibition effects on six pathogens comprising staphylococcus aureus, staphylococcus epidermidis, beta hemolytic streptococcus, escherichia coli, pseudomonas aeruginosa and candida albicans which can cause skin infection, and has antibacterial and insecticidal activities. The preparation method is reasonable and stable, short in production period, suitable for industrial production, and good in application prospect.

The invention provides an anthraquinone derivative, as well as a preparation method and application of the anthraquinone derivative serving as an antibacterial agent. The preparation method comprises the following steps: culturing a strain of Nigrospora sp. (HM565952) in a strain culture medium; fermenting the nigrospora sp. in a fermentation culture medium; filtering the fermentation broth, removing thallus, and extracting the fermentation broth with ethyl acetate; concentrating the extract, and performing chromatographic separation, and concentrating the eluent to obtain two types of yellow powder, namely 3,5,8-Trihydroxy-7-methoxy-2-methylanthracene-9,10-dione and austrocortirubin; and adding an acetic anhydride reagent in a solution dissolved with the compound, and reacting to obtain the anthraquinone derivative. The anthraquinone derivative obtained from the Nigrospora sp. (HM565952) has selective inhibitory activity to Bacillus subtilis, Bacillus cereus, Micrococcus tetragenus, Micrococcus lutea, Escherichia coli, Staphylococcus aureus, Staphylococcus albus, Vibrio parahaemolyticus and Vibrio anguillarum, and can be used for developing selective bacteriostatic agents; and the raw materials can be produced on a large scale, and not limited by resources. Therefore, the anthraquinone derivative has the advantage of wide application prospect.

The invention relates to a method for degradation of polychlorinated biphenyls by staphylococcus epidermidis. The method comprises the following steps: inoculating staphylococcus epidermidis in a polychlorinated biphenyl degradation culture medium and subjecting the staphylococcus epidermidis to the shaking culture at 28-32 DEG C for 24-120 hours; and analyzing the degradation effect of the bacterial strain on the PCBs after the culture is subjected to high performance liquid chromatograph determination. By the addition of extra carbon source glucose, the method can ensure that the staphylococcus epidermidis is more efficient in degrading the polychlorinated biphenyls, thereby allowing the bacteria to obtain the conditions for degrading the polychlorinated biphenyl.

The invention discloses a preparation method for a dichloro substituted II-type halogenated polyketone compound zunyimycin A, wherein the compound is derived from a secondary metabolite of a streptomyces sp.FJS31-2 strain (with the strain preservation number of CGMCC4.7321), and finally, a structure is identified and determined after strain activation, expansion culture and isolation. The compoundcan be used in treatment of diseases infected by gram-positive bacteria such as staphylococcus aureus (MRSA) and drug-resistant bacteria thereof, staphylococcus epidermidis, bacillus subtilis and thelike, is used in treatment of diseases infected by gram-negative bacteria such as super-spectrum beta-lactamase escherichia coli (ESBL), proteusbacillus vulgaris, bacterium burgeri and the like, andis used for treatment of diseases infected by fungi, such as candida albicans.

A protein isolated from Staphylococcus epidermidis having an approximate MW of 52 kD determined by SDS-PAGE and an N-terminal amino acid sequence (SEQ ID NO:1), and antigenic determinant-containing fragments of the protein, optionally coupled to an inert carrier or matrix, are disclosed. Disclosed are also a recombinant DNA molecule coding for the protein or the protein fragments; a vector comprising the DNA molecule or the corresponding RNA molecule; antibodies or antigen-binding peptides recognizing and specifically binding to the protein or protein fragment; use of the protein or protein fragment, or the vector, for the production of vaccines against Staphylococcal infections; use of the antibodies or antigen-binding peptides for the production of a medicament for passive immunization; a vaccine against Staphylococcal infections comprising the protein or protein fragment, or the vector, a medicament for passive immunization comprising the antibodies or antigen-binding peptides; and a method of prophylactic and / or therapeutic treatment of Staphylococcal infections.

The invention discloses a functional skin care product containing a paris polyphylla extract for treating acnes and a preparation method of the functional skin care product. The functional skin care product comprises the following raw materials in parts by weight: 50-70 parts of deionized water, 0.1 part of paris polyphylla extract, 0.5-1 part of prinsepia utilis royle oil, 1-5 parts of herba portulacae extract, 2-5 parts of glycerol, 3-5 parts of butanediol, 2-5 parts of 1,2-pentanediol, 2-8 parts of polydimethylsiloxane, 1-4 parts of nicotinamide, 1-2 parts of hydroxyethyl acrylate / sodium dimethyl acrylamide taurine copolymer, 0.1-0.5 part of acrylates (acrylic esters) C10-30 alkanol acrylate cross-linked polymer and 1-5 parts of silandiol salicylate. The functional skin care product contains the paris polyphylla extract, has the effect of resisting propionibacterium acnes, staphylococcus epidermidis and staphylococcus aureus, and is also good in safety without side and toxic effects.

The invention relates to a skin care product and a preparation method thereof. The skin care product is prepared by mixing plant extract, bifidobacterium adolescentis 8504 and oligosaccharide as mainraw materials together with deep-sea spring water. The invention has the beneficial effects that: the skin care product adopts natural raw materials, is prepared by compounding natural plant extract with bioactive probiotics and performing refining, is safer and healthier, has good moisturizing and absorbability, and is capable of permeating deep cortex, repairing skins after sun drying, lightening color spots, whitening and moistening skins, inhibiting pox and acne, resisting aging, reducing wrinkle depth, effectively reducing skin sensitivity, restoring skin elasticity, accelerating restoration of white and delicate skins, improving skin tolerance, removing acne, staphylococcus epidermidis and acarid, balancing oil secretion and removing acne marks.

A new fibrinogen binding protein or polypeptide originating from coagulase negative staphylococci, biotechnological methods for producing the protein or polypeptide having fibrinogen binding activity and a recombinant DNA molecule coding for the protein (or fragments thereof), and micro-organisms (including viruses) containing this recombinant DNA molecule. The present invention further comprises the therapeutic and diagnostic use of the protein and / or DNA, e.g., a diagnostic kit for determining the presence and / or type of coagulase negative staphylococci and a vaccine composition, comprising the protein or DNA.

The invention discloses a method for catalytic synthesis of a dicoumarin compound through lipases. The method comprises the step: 4-hydroxycoumarin is adopted as a substrate, and subjected to a Knoevenagel-Michael cascade reaction through lipase catalyzing to generate the dicoumarin compound with a compound in the formula II. The dicoumarin compound can be used for treating thrombus, resisting HIV, and treating asthma, skin cancer and snake venom, and can further be used for antimicrobial treatment of certain staphylococcus aureus and staphylococcus epidermidis and preparing of analytical reagents; and according to the method, reacting conditions are mild, aftertreatment is easy, the substrate is widespread, a catalyst can be recycled, and the method is an economical and environment-friendly new method for synthesizing the dicoumarin compound.

The invention discloses a molecular beacon and a kit for quickly identifying various clinically common bacteria and belongs to the technical field of microbiological detection. The sequences SEQ ID of the molecular beacon are as shown in v6p1, v6p2, v1p1 and v1r. Through comparison between various clinically common bacteria 16s ribosome RNA sequences in an NCBI gene sequence database and design of the molecular beacon at a tag sequence, clinically common 18 bacteria, including baumanii, A.hydrophila, burkholderia cepacia, citrobacter freundii, enterobacter cloacae, enterococcus faecium, enterococcus faecalis, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus mirabilis, pseudomonas aeruginosa, staphylococcus epidermidis, staphylococcus aureus, salmonella, serratia marcescens and stenotrophomonas maltophilia, can be quickly identified. The invention further discloses the kit containing the molecular beacon and used for detecting various bacteria, and the kit can quickly and accurately identify various clinically common bacteria.

The invention discloses a novel medicinal use of the active parts of abelmoschus manihot general flavones, in particular use of the active parts of abelmoschus manihot general flavones in the preparation of medicaments for treating urinary tract infection including cystitis and urethritis. Abelmoschus manihot has the effects of disinhibiting water, dissipating stasis, dispersing swelling, detoxification and relieving common symptoms of urinary tract infection, such as frequent micturition, urgent urination, odynuria and unwell micturition. The active parts of abelmoschus manihot general flavones have excellent inhibiting effects on staphylococcus aureus, staphylococcus epidermidis and other gram-positive bacteria and negative bacteria, and have anti-inflammatory and pain-relieving effects and an obvious diuretic effect. Therefore, the active parts of abelmoschus manihot general flavones have excellent effects on the treatment of urinary tract infection, particularly chronic recurrent urinary tract infection.

The invention discloses a novel actinomycin strain which generates actinomycin D: Streptomycesmutabilis TRM45540, preserved in U. S. Department of Agriculture Agricultural Research Service (ABS) Culture Collection on 14th, April, 2014 with the preservation number of NRRL B-59117. Meanwhile, the invention further discloses a seed medium which efficiently produces actinomycin D by using the strain, a fermentation medium and a preparation process of extracting and separating actinomycin D from fermentation liquor. An antibacterial activity test shows that the strain and the actinomycin D generated by fermentation of the strain have broad-spectrum and efficient antagonistic activity on pathogenic bacteria such as cotton verticillium and fusarium wilts, walnut pythium ultimum, staphylococcus aureus, staphylococcus epidermidis, candida albicans and the like and have an important application value and a very good application and development prospect on safe production of crops such as cotton, walnuts and the like and in preventing and controlling diseases such as bovine mastitis, women's vaginitis and the like.