The invention relates to an on-site fermented bacillus licheniformis preparation for aquaculture farm and applications thereof. The preparation comprises the following raw materials: bacillus licheniformis powder, colistin sulfate, natamycin, inosine, corn flour, corn steep liquor powder, sodium chloride, serine, fructose, and brown sugar. The colistin sulfate and natamycin in the preparation can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (inosine, fructose, and serine) can rapidly promote the germination of spores of bacillus licheniformis and improve the germination rate of spores. The provided preparation can be applied to the onsite fermentation and propagation in a simple and crude aquaculture farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The preparation can improve the water quality and intestinal functions, prevent disease, promote growth, increase output, and improve product quality.