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Lactobacillus salivarius M6 and antibacterial composition containing same

A technology of Lactobacillus salivarius and composition, applied in the direction of medical preparations containing active ingredients, bacteria, microorganisms, etc., can solve problems such as unfavorable health, weakened intestinal function, diarrhea, dehydration, etc., to increase ability and improve intestinal tract The number of probiotics and the effect of inhibiting the growth of pathogenic bacteria

Active Publication Date: 2012-03-14
SYNGEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, the administration of antibiotics in animals not only kills the pathogenic bacteria in the intestinal tract, but also kills the probiotics, greatly weakening the intestinal function, easily leading to diarrhea, dehydration and other phenomena, which is not conducive to health

Method used

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  • Lactobacillus salivarius M6 and antibacterial composition containing same
  • Lactobacillus salivarius M6 and antibacterial composition containing same
  • Lactobacillus salivarius M6 and antibacterial composition containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Inhibition of Escherichia coli and Salmonella by Lactobacillus salivarius

[0034] 1. Filter paper agar diffusion method

[0035]Lactobacillus salivarius M6 was screened from the intestinal tract of animals, and its culture characteristics are as follows: gray colonies with a diameter of 2-3 mm grow on MRS (Difco, REF288130) agar plates, and the optimum culture temperature is 37°C.

[0036] Take a colony of Escherichia coli (BCRC 11634) and Salmonella (BCRC12947) of Platinum ear and inoculate it on the slant of TSA medium (Soybean-Casein Digest Agar Medium, DifcoTM, REF236950), cultivate it at 35-37°C for 17-24 hours, and use spectroscopic The turbidity measurement is carried out with a photometer, and the appropriate turbidity of the bacteria is adjusted (80% of the turbidity at about 600 nm, 80% T). Absorb 0.5mL of Escherichia coli and Salmonella bacterial solution into TSA medium at 48°C and mix evenly, then quantitatively pour 15mL into the petri dish, le...

Embodiment 2

[0046] Embodiment 2 animal test

[0047] 1. Effect of Lactobacillus salivarius M6 on growth performance of nursery pigs

[0048] 36 weaned pigs (weaned age: 25 days) were randomly assigned according to sex to the control group, antibiotic group or Lactobacillus salivarius M6 group, with 12 pigs in each group. The main ingredients of the feed were corn and soybean flour. The diet of the control group did not contain carbadox and Lactobacillus salivarius M6, the feed of the antibiotic group was supplemented with carbadox, and the feed of the Lactobacillus salivarius M6 group was supplemented with Lactobacillus salivarius M6. During the test period, free food and free drinking water were adopted, and the test was carried out for 28 days to compare the growth performance of piglets, the number of bacteria and diarrhea. Each pig was weighed at the beginning and end of the experiment, and the feed intake of each group was recorded to calculate the daily gain and feed conversion rat...

Embodiment 3

[0055] Example 3 Lactobacillus salivarius M6 and other lactic acid bacteria are compared for the tolerance of commonly used animal antibiotics

[0056] The antibiotic susceptibility test was used to analyze and compare the tolerance of Lactobacillus salivarius M6 and other lactic acid bacteria to antibiotics. The smaller the inhibition zone, the better the tolerance of this lactic acid bacteria to antibiotics. After Lactobacillus salivarius M6 and other lactic acid bacteria were cultured with MRS based on anaerobic culture at 37°C, 100 μL of each was evenly spread on the MRS agar. After standing still for 20-30 minutes, place a 6mm sterile filter paper, add 5 μL of three antibiotics commonly used in animal feed, place it in anaerobic culture at 35-37°C for 16-24 hours, and measure the size of the inhibition zone (mm ), and the measurement results are shown in Table 3. When the concentration of aureomycin is 10μg / Disc or 30μg / Disc, Lactobacillus salivarius M6 has the smallest ...

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Abstract

The invention provides Lactobacillus salivarius M6, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the number of CGMCC 3505. The invention also provides a lactobacillus which can be mixed with animal feed or added into water, can be used together with antibiotics at the same time, and has antibiotic resistance. The strain can inhibit growth of Salmonella enterica, Escherichia coli and other pathogenic bacteria. The antibacterial composition of the Lactobacillus salivarius M6 can be used in drinking water, drinking water additives, feed and food additives of animals, human and animal medical composition, as well as drinks, drinking additives, foods, food additives and the like of humans. By implementation of the invention, pathogenic bacterium infection of animals or humans can be effectively prevented and treated, and the number of intestinal probiotics can be increased or maintained, so that the growth of pathogenic bacteria is inhibited, the dosage of antibiotics is reduced, and the aim of preventing disease infection of animals and humans can be achieved.

Description

technical field [0001] The invention relates to an antibacterial composition containing Lactobacillus salivarius M6 strain, which has the effect of inhibiting the growth of pathogenic bacteria such as Escherichia coli and Salmonella enterica. Background technique [0002] Probiotics generally refer to live bacteria that can improve the balance of endophytic microorganisms and benefit the host when applied to the human body or organisms. Common types of probiotics include Lactobacillus, Bifidobacterium, and Streptococcus, and lactic acid bacteria are the most commonly used strains in recent years. Lactic acid bacteria refers to the general term for bacteria that can metabolize carbohydrates and produce more than 50% lactic acid. It is beneficial to intestinal health. Bifidobacterium spp. and Lactobacillus spp. are also used clinically to treat and prevent diarrhea, infant allergies, and ulcers Colitis and other related studies, among which, animal experiments have proved tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16A23L1/30A23L2/52A61K35/74A61P31/04C12R1/225A61K35/747
CPCY02A50/30
Inventor 魏钰珊戴芳云
Owner SYNGEN BIOTECH
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