A kind of petroleum degrading bacterial agent and preparation method thereof

A technology of oil-degrading bacteria and strains, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as pollution, achieve environmental safety, simple and economical preparation process, and convenient transportation and storage

Active Publication Date: 2019-09-17
NORTH CHINA SEA ENVIRONMENTAL MONITORING CENT OF STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies in the prior art, and provide a kind of petroleum degrading bacterial agent and preparation method thereof, aim at effectively remediating seabed petroleum pollution through bioremediation, improve petroleum degradation ability, and solve the problem of petroleum pollution provide a breakthrough

Method used

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  • A kind of petroleum degrading bacterial agent and preparation method thereof

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Embodiment 1

[0034] A preparation method of a petroleum degrading bacterial agent, comprising the following steps:

[0035] (1) The strains of Caulobacter ineruis, Candida parapsilosis, Brevibacterium phenol-resistant, Arthrobacter globosa, and Septoria salinii were expanded to a cell concentration of 10 9 ~10 10 cfu / g, mix Caulus ineruis, Candida parapsilosis, Brevibacterium phenol-resistant, Arthrobacter globosa and Septoria salinii according to the mass ratio of 3:2:2:1:1 to obtain the mixed bacteria kind;

[0036] (2) Autoclaving the liquid fermentation medium for 15-30min, in the aseptic operating table, inoculate the mixed strains obtained in step (1) into the sterilized liquid fermentation medium, the mixed strains and the liquid fermentation medium The weight ratio is 1:12, and the liquid fermentation medium inoculated with the mixed strains is placed in a constant temperature water bath shaker, and fermented continuously for 60 hours at 140rpm and 27°C;

[0037] (3) Centrifuge ...

Embodiment 2

[0044] A preparation method of a petroleum degrading bacterial agent, comprising the following steps:

[0045] (1) The strains of Caulobacter ineruis, Candida parapsilosis, Brevibacterium phenol-resistant, Arthrobacter globosa, and Septoria salinii were expanded to a cell concentration of 10 9 ~10 10 cfu / g, mix Caulus ineruis, Candida parapsilosis, Brevibacterium phenol-resistant, Arthrobacter globosa and Septoria salinii according to the mass ratio of 3:2:2:1:1 to obtain the mixed bacteria kind;

[0046] (2) Autoclaving the liquid fermentation medium for 15-30min, in the aseptic operating table, inoculate the mixed strains obtained in step (1) into the sterilized liquid fermentation medium, the mixed strains and the liquid fermentation medium The weight ratio of the mixture is 1:10, and the liquid fermentation medium inoculated with the mixed strains is placed in a constant temperature water bath shaker, and fermented continuously for 55 hours at 140rpm and 28°C;

[0047] ...

Embodiment 3

[0054] A preparation method of a petroleum degrading bacterial agent, comprising the following steps:

[0055] (1) The strains of Caulobacter ineruis, Candida parapsilosis, Brevibacterium phenol-resistant, Arthrobacter globosa, and Septoria salinii were expanded to a cell concentration of 10 9 ~10 10 cfu / g, mix Caulus ineruis, Candida parapsilosis, Brevibacterium phenolicus, Arthrobacter globosa and Septoria salinii in a mass ratio of 3:2:2:1:1 to obtain the mixed bacteria kind;

[0056] (2) Autoclaving the liquid fermentation medium for 15-30min, in the aseptic operating table, inoculate the mixed strains obtained in step (1) into the sterilized liquid fermentation medium, the mixed strains and the liquid fermentation medium The weight ratio is 1:15, and the liquid fermentation medium inoculated with the mixed strains is placed in a constant temperature water bath shaker, and fermented continuously for 48 hours at 140rpm and 30°C;

[0057] (3) Centrifuge and remove the wat...

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Abstract

The invention relates to a petroleum-degrading bactericide and also relates to a preparation method of the petroleum-degrading bactericide. The preparation method of the petroleum-degrading bactericide comprises the following steps: (1) performing enlage cultivation on such bacteria as caulobacter segnis, candida parapsilosis, brachybacterium phenoliresistens, arthrobacter globiformis and sporidiobolus salmonicolor till the concentration of the bacteria is 10<9>-10<10> cfu / g, and uniformly mixing according to the mass ratio of 3: 2: 2: 1: 1 to obtain a mixed culture; (2) fermenting the mixed culture in a liquid fermentation culture medium for 48-60 h; (3) carrying out centrifugal separation to obtain thick strain cells; (4) uniformly mixing the thick strain cells with a 0.9% NaCl solution and a sea mud leachate; (5) adding nutrient substances, and then preserving at 4 DEG C in a sealed manner. The petroleum-degrading bactericide is high in petroleum degradation rate.

Description

technical field [0001] The invention belongs to the technical field of petroleum degradation, and in particular relates to a petroleum-degrading bacterial agent; the invention also relates to a preparation method of the above-mentioned petroleum-degrading bacterial agent. Background technique [0002] With the increasing development of oil development, shipping and oil industry, marine oil spill accidents occur frequently, and oil pollution has brought serious harm to the marine environment and marine ecosystem, and can directly or indirectly affect the survival and sustainability of human beings. Continuous development. According to the United Nations Environment Program report, 2 to 20 million tons of oil flow into the sea every year, and oil pollution has become a worldwide pollution problem. The oil spill in the Gulf of Mexico in 2010 was one of the most serious marine pollution incidents in the history of the United States, and the ecological damage and economic losses...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16B09C1/10C12R1/72C12R1/06C12R1/01C12R1/645
Inventor 赵升韩龙江耿晓潘玉龙刘欣禹周瑞佳
Owner NORTH CHINA SEA ENVIRONMENTAL MONITORING CENT OF STATE OCEANIC ADMINISTATION
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