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Yeast strain for producing biosurfactant and application thereof

A yeast strain and yeast technology, applied to yeast and its application field, can solve problems such as the inability of the emulsion to exist stably, and achieve the effects of not easy to mutate, fast growth, and simple cultivation method

Active Publication Date: 2013-09-11
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biosurfactants are small molecules with low molecular weight, and the emulsions they form often cannot exist stably

Method used

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  • Yeast strain for producing biosurfactant and application thereof
  • Yeast strain for producing biosurfactant and application thereof
  • Yeast strain for producing biosurfactant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Isolation and purification of yeast strains

[0053] 1. Medium preparation

[0054] (1) Basic medium:

[0055] (NH 4 ) 2 SO 4 1g, K 2 HPO 4 0.8g, KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 ·2H 2 O 0.1g, FeSO 4 ·7H 2 O 5mg, 1mL filtered sterilized vitamin solution, 1,000mL purified water, pH 5-6, the culture medium was sterilized at 121°C for 20 minutes.

[0056] The composition of the vitamin solution is as follows: niacin 100mg, vitamin B1 100mg, biotin 5mg, para-aminobenzoic acid 50mg, vitamin B12 1mg, calcium pantothenate 50mg, vitamin B650mg, microbiotic M 50mg, 3Na EDTA 200mg, diluted with sterile purified water To 100mL.

[0057] (2) Enrichment medium:

[0058] Add 0.2% phenanthrene solution to the basic medium, adjust the pH to 5-6, and sterilize the medium at 121°C for 20 minutes; the composition of the phenanthrene solution is: 1g phenanthrene, 1L n-hexane.

[0059] (3) Separation and purification medium:

[0060] Add 0.2% phenanthrene solution and 2% agar to th...

Embodiment 2

[0083] Example 2 Study on the microbiological characteristics of yeast strains

[0084] 1. Observation of colony morphology

[0085] The purified yeast strains were streaked in YPD solid medium and cultured at 27°C until colonies grew, and the morphology of the colonies was observed. The results are as follows:

[0086] The colony characteristics of the strain are: the diameter of the colony after 2 days of culture on the YPD plate is 5-6mm, the colony is round, the surface is rough, the edges are irregular, prominent, and pink.

[0087] 2. Cell morphology observation

[0088] The strains were inoculated into YPD medium and cultured at 27°C and 150rpm for 3 days. The cell morphology of the strains in the culture medium was observed with a microscope. The observation results were as follows figure 1 As shown, the yeast cells are elliptical, the long axis of mature cells is 7-8um, and the short axis is 3-4um.

[0089] 3. 26S rDNA gene sequence determination

[0090] The yeast was inoculated...

Embodiment 3

[0094] Example 3 Cultivation of Salmonicolor S. salmonicolor AH3

[0095] 1) Prepare the inoculation mother liquor of yeast strain S. salmonicolor AH3

[0096] A fresh slant strain of yeast strain S.salmonicolor AH3 was inoculated into a naphthalene medium, which is the sole carbon source for the growth of yeast strain S.salmonicolor AH3, cultured at 27°C, 150rpm, and the OD of the culture solution was measured. 600 Value until OD 600 When the value reaches 0.5, an inoculation mother solution of S. salmonicolor AH3 strain is obtained, in which one loop of S. salmonicolor AH3 strain is inoculated into every 100 ml of naphthalene medium.

[0097] 2) Cetane medium culture

[0098] The inoculation mother liquor of the strain S. salmonicolor AH3CGMCC No. 4814 was inoculated into hexadecane medium, where the inoculum amount was 1% (v / v), that is, the ratio of the volume of the inoculation mother liquor to the hexadecane medium was 1: 100, then in the dark, incubate at 27℃, 150rpm, and measu...

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Abstract

The invention discloses a yeast strain Sporidiobolus salmonicolor AH3 for producing a biosurfactant. The microbial collection number of the yeast strain is CGMCC No. 4814. The yeast strain provided by the invention can grow with C10-C24 alkane and / or polycyclic aromatic hydrocarbons as the carbon source, and the biosurfactant is generated. When the yeast strain is cultured with alkane and polycyclic aromatic hydrocarbons as the carbon source, emulsifying ability of the generated biosurfactant is enhanced. The yeast strain Sporidiobolus salmonicolor AH3CGMCC No.4814 has good effects of degrading and emulsifying hydrophobic organic matters, and has a good application prospect in treatment of petroleum produced wastewater.

Description

Technical field [0001] The invention relates to yeast in the field of environmental biology and its application, in particular to a yeast strain producing surfactant and its application in oil extraction wastewater treatment, belonging to the field of yeast strains. Background technique [0002] Oily wastewater mainly comes from petroleum, petrochemical, steel, coking, gas generating stations, mechanical processing and other industrial sectors. The oily substances contained in it include natural petroleum, petroleum products, tar and its fractions, as well as edible animal and vegetable oils and fats Class etc. If the oily wastewater generated in the production process of these industrial sectors is not recycled, it will cause a great waste of resources; if it is directly discharged into rivers, lakes or bays without treatment, it will pollute the water body, and the hydrophobic organic matter in the oily wastewater will be An oil film is formed on the surface of the water body,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P1/02A62D3/02C02F3/34C12R1/645C02F101/30A62D101/20
Inventor 张昱邓艳芹杨敏黑山姆吕文洲
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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