100results about How to "Not easy to mutate" patented technology

The invention relates to a method for making landscape plant waste compost inoculum and an application of the same. The method is as follows: bacillus subtilis, trichoderma viride, lactobacillus plantarum, paracoccus denitrification, aspergillus oryzae and saccharomyces cerevisiae respectively occupying 10 to 20 mass portions are inoculated in a solid state fermentation medium so as to carry out cultivation and fermentation in dark sealing for 1 to 3 weeks at a temperature of between 25 and 30 DEG C; one week later, 250 mass portions of saw dust is added and evenly stirred and then cultivation and fermentation are continued; two weeks later, hay is placed to absorb surplus moisture and then is removed, and powdery inoculum can be obtained through drying in the shade for further use. Garden wastes are piled up and then are inoculated with inoculum with the weight occupying 1 to 3 per mill of that of a pile and dipped in warm water at a temperature of between 30 and 40 DEG C; compost is carried out at a temperature of between 55 and 65 DEG C, and pile turning and water replenishing are carried out every 3 to 4 days; then, pile turning and water replenishing are carried out every two weeks so as to keep pile water content of between 50 and 60 percent; thus, compost is completed after three weeks so as to reach to rotten innocuity standards. The strain has simple making and low cost, and can be directly used or bred continuously; moreover, the strain has no variation, no inactivation and long storage period, and the obtained compost can improve soil and increase nutrient utilization rate.

The invention relates to a chimeric antigen receptor based on CD20, and applications thereof, and more specifically relates to a cell technology construction method of chimeric antigen receptor T (CAR-T) taking tumor specific target spot CD20 as a base, and applications of the chimeric antigen receptor T in treatment of tumor. The chimeric antigen receptor T is composed of an antigen binding domain, a transmembrane domain, a costimulatory signal transduction region, and a CD3 zeta signal transduction domine via series connection; wherein the antigen binding domain is used for binding tumor surface antigens, and the tumor surface antigen is CD20. According to applications, specific gene modification of single-chain antibody of tumor surface antigen CD20 is carried out, the modified antibody is capable of increasing antigen-antibody binding force, mutation is not easily caused. Compared with other chimeric antigen receptors and other tumor antigens, the chimeric antigen receptor possesses following advantages: the effect is better, target spot expression quantity is higher, immune effect on CAR-T cells is improved, and treatment effect of CAR-T cells is improved.

A molten glass supply device is provided, which can solve unavoidable problems for high viscosity characteristics in connection with the conventional molten glass supply device for high viscosity glass. Such problems include improperly high heating cost caused by excessive heat radiation in a melting furnace, reduction in the grade of products deriving from an excess amount of an erosion foreign material and reduction in the product yield. High viscosity molten glass having a property in which a temperature at which the molten glass exhibits a viscosity of 1000 poise is 1350 DEG C. or higher is supplied to a forming device through a melting furnace, a distribution portion in communication with the outlet of the melting furnace, and a plurality of branch paths branching from the distribution portion. In the branch paths, distribution resistance providing portions that provide distribution resistance to molten glass passed through the branch paths are provided. The supply pressure of the molten glass is equalized when molten glass is distributed from the distribution portion to the branch paths. The distribution portion has a shallower bottom than the melting furnace.

The invention discloses a spine-free zanthoxylum bungeanum cultivation method, which comprises land selecting and preparation, seedling raising, ear cutting and grafting, field planting, cultivation management, and seedling raising of spine-free zanthoxylum bungeanum, wherein a seed stirring agent is used during the seedling raising process, and during the ear cutting and grafting, the scion male parent is soaked with an essence liquid so as to improve the healing and scion survival rate of the joint part. According to the present invention, with the grafting of more than three generations, the spine-free or micro-spine characteristic can be stabilized, and the new grafted seedling has characteristics of high survival rate, strong branches, convenient transplanting and good quality.

The invention provides a complex microbial agent for stabilizing treatment of stock garbage in cities and towns as well as a preparation method and an application of the complex microbial agent. The complex microbial agent contains sporotrichum thermophile, pseudomonas alcaligenes, halobacillus sp, bacillus subtilis, pseudomonas putida, candida utilis, saccharomyces cerevisiae, phanerochaete chrysosporium and lactobacillus plantarum. Growth metabolism, degradability and activity of all microbial strains in the complex microbial agent can effectively complement and cooperate with one another, the microbial strains can grow and propagate quickly in a landfill system to promote the temperature of a garbage treatment environment to increase quickly, meanwhile, organic matter, BDM and toxic andharmful substances in a landfill can be quickly and efficiently degraded, the stabilizing time of the garbage landfill is greatly shortened, stable harmless treatment of landfill is promoted, and thecomplex microbial agent can be applied to aerobic stabilizing treatment of garbage in cities and towns in practice.

The invention relates to a levalbuterol hydrochloride orally disintegrating tablet and the preparation method; wherein the levalbuterol hydrochloride orally disintegrating tablet comprises an active pharmaceutical ingredient, the levalbuterol hydrochloride, and pharmaceutical necessities. The pharmaceutical necessities include a disintegrant, a filling agent, a masking agent and a corrective. The raw material ingredients calculated by weight ratio are 0.79-2.5 per cent levalbuterol hydrochloride, 50-60 percent disintegrant, 20-40 per cent filling agent, 1.58-10 per cent masking agent and 10-12 per cent corrective, which are made into the orally disintegrating tablets by direct powder compression process. The tablets of the invention is taken easily without water, disintegrated quickly and takes effect quickly, high in bioavailability, less stimulatory function to gastrointestinal tract, and applicable to prevention and cure of bronchial asthma, asthmatic bronchitis, and bronchospasm of an emphysema patient.

The invention relates to a novel microorganism flora mixture and a mixed nutrient medium thereof. The nutrient medium is filter liquor which is prepared by taking plant powder, mineral composition, animal powder, organic acid and the like as raw materials, adding sewage or comprehensive sewage in cities to be processed, mixing, fermenting and filtering. The microorganism flora mixture is a microorganism preparation which is prepared by the following steps: respectively selecting fusarium oxysporum, aspergillus fumigatus, sphaerotilus, vorticella microstoma, photosynthetic bacteria, methanebacteria, desulphovibrio, nitrobacterium, pseudomonas fluorescens and other bacterial strains from obligate aerobes group, anaerobic bacteria group and facultative aerobe group preferentially, then expanding and cultivating, inoculating into the above mixed nutrient medium, mixing, cultivating and domesticating for 15-30 days at the temperature of 20-45 DEG C. The mixed nutrient medium in the invention can cultivate, propagate and domesticate flora mixture with the combination advantages of flora; the flora has stable integrity, no easy variation and strong adaptability, and the invention breaks through the technical bottlenecks of easy degeneracy of microorganism flora, easy decrease of biological activity and slow proliferation and expansion.

The invention discloses a tissue culture rapid propagation method of eucalyptus cloeziana. The method adopts an excellent single plant of an excellent eucalyptus cloeziana family, which is cut down and promoted to germinate, a bud serves as an explant, a stem section with a resting bud induces a sterile bud in vitro, the sterile bud is obtained without a callus seedling path, the stem section of the explant directly grows a side bud or an adventitious bud, and the sterile bud is subjected to subculture enrichment culture, rooting culture and rooting seedling transplanting to obtain a regenerated plant. The tissue culture rapid propagation method of eucalyptus cloeziana is lower in operation difficulty, stable in the growth of a sterile bud propagation system and good in repeatability and can be used for scale expanding propagation repeatedly, after the rooting culture of cut buds, the rooting rate is higher, the popularization value is realized, rooting seedlings can survive after transplanting, seedlings normally grow, the obtained regenerated plant can robustly grow in a large field, a survival rate of afforestation is more than 95% and excellent inheritable characters of the selected plant can be kept.

The invention discloses an enterobacter cloacae strain and applications of the enterobacter cloacae strain. The enterobacter cloacae strain is assigned with the accession number of CGMCC No.12819. The invention discloses the enterobacter cloacae which is obtained by separating the soil at the root of tobacco and can efficiently degrade the bactericide, namely, metalaxyl. The experiment proves that enterobacter cloacae can grow in an LB culture medium by taking metalaxyl as the unique carbon source and energy, and can degrade metalaxyl; under the liquid culture condition with added exogenous nutrients, enterobacter cloacae can degrade 90% or above of metalaxyl with the high concentration being 5mg / L mixed in the culture medium within 48h, and has good degrading effect within the wide pH range. With the degrading fungicide produced by adopting the technical scheme provided by the invention, the metalaxyl pesticide residue in the water body and soil and on the surfaces of tobacco leaves can be efficiently degraded, so that the ecological environment is protected and repaired, and the technical gap of degrading the metalaxyl pesticide residue by adopting enterobacter cloacae at present is made up.

The invention provides an organoid technology-based individualized lung cancer primary cell culture method. According to the method, high-throughput screening is carried out tumor cell models in vitrothrough organoid models established in tumor tissues of patients, so as to instruct individualized treatment of the patients. Specifically, excised lung cancer fresh tissues are utilized to carry outorganoid culture to establish individualized lung cancer primary cell models and carry out authentication, so that basis is provided for accurate treatment evaluation of lung cancer.

The present invention discloses a fluorescent quantitative test strip for the porcine epidemic diarrhea virus (PEDV), and a preparation method and application thereof. The fluorescent quantitative test strip comprises a sample pad, a fluorescent label binding pad sprayed with a fluorescent label probe, a nitrocellulose membrane sprayed with a detection line T and a quality control line C, and a piece of absorbent paper that are arranged sequentially in the order of connection. The fluorescent quantitative test strip further comprises a plastic bottom liner arranged in the lower part. The plastic bottom liner is configured to provide an assembly platform; the fluorescent label binding pad is sprayed with a PEDV monoclonal antibody fluorescent microsphere label and a goat-anti-rabbit IgG fluorescent microsphere label; and the nitrocellulose membrane is sprayed with a detection line T formed by the PEDV monoclonal antibody and a quality control line C formed by the goat-anti-rabbit IgG polyclonal antibody. Experiments have proved that by using the test strip provided by the present invention, the sensibility is at least 10 times higher than that by using the existing RT-PCR and colloidal gold test strip detection method, the test sample is initially quantified, and the test result is digitized, so that the human subjective bias is avoided, and a technical means is provided for early diagnosis of the PEDV.

A sensor and manufacture method of the sensor. The manufacture method comprises following steps: forming an active element on a substrate; forming a first insulation layer on the substrate for covering the ative element, wherein the first insulating layer has a first opening for locally exposing the active element; forming a blanket-covering type conductive layer made of conductive materials on the first insulation layer, wherein the blanket-covering type conductive layer is connected with the active element via the first opening; forming a photoelectric conversion material layer on the blanket-covering type conductive layer; forming a first photoresist pattern on the photoelectric conversion material layer, the first photoresist pattern serving as a mask; patterning and converting the photoelectric conversion material layer to a photoelectric conversion unit; patterning the blanket-covering type conductive layer to form a first electrode, wherein the first electrode is arranged in the first opening and enables the photoelectric unit to be electrically connected with the active element.

The invention disclosed a way of preserving the big fungus strain for a short term. It comprises the following steps: (1) inoculating the strain to the solid medium and cutting the medium into several pieces when the hyphal body nearly covered the whole flat plate; (2) adding sterile distilled water to the container first, then putting the pieces of medium in the container; covering the lid and preserving it at 4 Deg C. The invention can preserve the strain for a short term without variation; besides, it's very simple and economical.

The invention relates to phlebia acerina strain and application thereof in degrading metalaxyl pesticide residue, and belongs to the biological technical field of organisms. The strain is utilized for degrading high-concentration metalaxyl pesticide residue. According to the identification, the scientific name of the white-rot fungi is phlebia acerina Y3667 which is stored in the Common Organism Center of the China Committee for Culture Collection of Microorganisms, wherein the storage number is CGMCC 6809; the ITS (Internal Transcribed Spacers) sequences are extracted by the DNA, cloned and measured; compared with the stored sequences in GenBank, the homology of the phlebia acerina strain with the phlebia acerina strain BMC3014 is 99%. The strain is cultivated in liquid medium to obtain microbial inoculum; and the microbial inoculum is added with the high-concentration metalaxyl pesticide for effectively degrading the metalaxyl pesticide residue. The degrading microbial inoculum generated by the technical scheme in the invention can be used for effectively degreasing the metalaxyl pesticide on the surface of water and agricultural products, so that the ecological environment is protected and restored.

The invention discloses a soil improvement bactericide and a preparation method thereof. Wormcast, humic acid crude powder, potassium hydroxide and microbial cultures are used as main materials, and a wetting dispersant is used as an auxiliary material. The invention belongs to the technology of cycle of organic fertilizers and resources of agricultural wastes. The soil improvement bactericide prepared by adopting the preparation method is high in organic content, strong in biological activity of culture and complete in nutrition, and has the comprehensive effects of increasing roots, strengthening seedling, resisting stress, preventing diseases, resisting lodging, promoting precocity, resisting residue, preventing pollution, increasing yield and improving the soil quality. The soil improvement bactericide is simple in preparation process, low in production cost, easy to operate, agile to produce, convenient to use and easy to popularize and apply in a large area.

The invention discloses a rapid nonsexual systematic mating of Herba Epimedii, which is performed as following: A, forming ridge in greenhouse or plastics greenhouse and paving muck soil with thickness of 10-15cm;B, choosing plants in good health and free from disease, removing overground leaves, taking underground stem part, cutting at the position capable of tillering bud into pieces of 2-3cm with at least on tillering oculus to obtain seedlings; C, soaking in clean water for 1-2h, seeding with spacing in the rows and between the rows of 5cmX5cm-10cmX10cm and sowing depth of 5-8cm, covering soil, irrigating rooting water, and overlaying film; D, ripping away the film after sprouting, watering, weeding, and transplanting after 2-4 months. The invention can maintain properties of the plants. Progenitive first filial generation of Herba Epimedii has stable properties and high survival rate. The invention has simple method and wide application, and is suitable for popularization in countryside.

The invention relates to the technical field of tissue culture, in particular to a morinda officinalis tissue culture method. The method comprises the following steps of taking a disinfected and cleaned morinda officinalis rattan as an explant; inoculating the explant to an axillary bud induction culture medium, and illuminating the explant every day until axillary buds grow out; inoculating the axillary buds to a cluster bud induction culture medium, and illuminating the axillary buds every day until cluster buds grow out; and inoculating the cluster buds to a rooting induction culture medium, and illuminating the cluster buds every day until root systems grow out. According to the morinda officinalis tissue culture method, the rattan of the morinda officinalis is adopted as the explant for tissue culture, namely, a direct organogenesis way of the morinda officinalis is used, a callus stage is not needed, good hereditary stability is achieved, variation is not prone to occurring, and the specific medicinal characters of the morinda officinalis can be effectively kept.