The invention discloses a method for obtaining an excellent clone through
fiber bamboo subterraneous stem in-situ multiple
mutagenesis. The method comprises the steps that a subterraneous stem of
fiber bamboo is selected; an internal
irradiation mutagenic agent is selected to be prepared into mutagenic agent solutions separately; a chemical mutagenic agent
ethylmethane sulfonate is selected to beprepared into mutagenic agent solutions separately; a stainless steel continuous
syringe is used for filling different segments of the selected subterraneous stem of the
fiber bamboo with the internalirradiation mutagenic agent of different concentrations, and injection ports are sealed by non-sticking
adhesive tapes; after 30-40 days, the stainless steel continuous
syringe is used for filling the different segments of the selected subterraneous stem of the fiber bamboo with the chemical mutagenic agent solution, and the injection ports are sealed by non-sticking
adhesive tapes; after earthing is performed for 60-90 days, different mutagenic buds on the segments, subjected to multiple treatment of the internal
irradiation mutagenesis and mechanical
mutagenesis, of the subterraneous stem are germinated into bamboo, and through screening and underground root dividing propagation, the clonal new
germplasm or new species of the fiber bamboo with excellent traits.