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Method for quick breeding royal waterplatter

A rapid and multiplication medium technology, applied in horticultural methods, botanical equipment and methods, plant phenotype improvement, etc., can solve the problems of low seed setting rate, low maturity, and inability to complete self-propagation and self-breeding.

Inactive Publication Date: 2005-03-02
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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Problems solved by technology

This condition restricts the growth and reproduction of Wang Lian in the area north of the Yangtze River in my country, that is, Wang Lian can only be cultivated as an annual plant in these areas, and the seed setting rate is low and the maturity is not high, so it cannot complete self-propagation and self-fertilization. Market demand

Method used

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Embodiment Construction

[0033] In order to propagate the king lotus seedling rapidly, the present invention carries out according to the following steps:

[0034] (1) getting the stem tip of 0.5~1.0 centimeters of lotus regia as the culture of lotus regia in length;

[0035] (2) Rinse the Wang Lian culture with running water for 20 to 30 minutes, then scrub the surface of the Wang Lian culture with 75% alcohol cotton balls, then soak it in 0.1% mercuric chloride for 6 to 8 minutes, and rinse with sterile water. Rinse for 8-10 minutes;

[0036] (3) Insert the Wang Lian culture treated by step (2) into the induction medium, cultivate for 20 to 30 days, and the Wang Lian culture begins to germinate and grow; the induction medium is: add 0.5 mg BA (6-benzyl adenine), 0.02 mg NAA (naphthalene acetic acid), 0.3 mg GA 3 (Gibberellin), 2 grams of activated carbon, 30 grams of sucrose, 6 grams of agar; wherein each liter of MS basic medium contains:

[0037] NH 4 NO 3 (ammonium nitrate) 1650mg

[0038] ...

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Abstract

The present invention discloses the quick breeding method of royal waterplatter. The stem tip of royal waterplatter as the cultured matter is cultured through disinfection, inducing culture, differentiation, proliferation and rooting culture to propagate royal waterplatter quickly. Over 10,000 seedlings of royal waterplatter may be cultured in one year with one underground stem tip of royal waterplatter. The present invention makes it possible to provide great amount of royal waterplatter seedlings for decorating water scenes.

Description

Technical field: [0001] The invention relates to a plant tissue culture method, more specifically to a rapid propagation method of Wang Lian, which belongs to the application of biotechnology in the production of plant seedlings. Background technique: [0002] King Lily (Victoria cruziana D.orbigny) belongs to Nymphaeaceae King Lily is a large-scale rare ornamental plant, native to the Amazon River Basin in South America, and is a typical tropical perennial aquatic floating leaf plant. Wang Lian is known as the "King of Water Flowers" because of its strange and huge leaf shape, strong fragrance of flowers, and three colors of white, red, and purple during the blooming process. It is of high ornamental value. However, it has strict requirements on growth conditions, especially air temperature and water temperature. Only when the air temperature and water temperature are stable above 25°C, can Wang Lian grow safely in the open-air pool, bloom and set seeds. This condition re...

Claims

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Application Information

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IPC IPC(8): A01H3/04A01H4/00
Inventor 徐立铭刘艳玲李洪林
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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