Fast propagation process of potarnogeton lucens

A fast technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can not solve problems such as large-scale production, low reproduction coefficient, and difficulty in meeting the needs of water body vegetation restoration.

Inactive Publication Date: 2007-03-21
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

[0003] In nature, the reproduction coefficient is low. If the conventional division method is used for reproduction, large-scale production cannot be formed, and it is difficult to meet the needs of water body vegetation restoration.

Method used

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Embodiment Construction

[0016] The following is a detailed description in conjunction with the embodiments:

[0017] 1. The steps ①

[0018] The underground stem tip of P. glabra with a length of 1 to 2 cm was used as the culture of P. glabra.

[0019] 2. The steps ②

[0020] After washing the P. glabra culture with running water for 30-50 minutes, take it out and scrub the surface of P. glabra culture with 75% alcohol cotton ball, and then soak the culture with 0.1% mercuric chloride for 5-8 Minutes, then take it out and rinse with sterile water 4-6 times, each 2-4 minutes;

[0021] 3. The steps ③

[0022] Put the P. glabra culture treated by step ② into the induction medium, culture temperature 23~25℃, light for 10-12h / d, light intensity 1000Lx; cultivate for 20-30 days, P. glabra The culture begins to germinate and grow;

[0023] The induction medium is: 0.1 mg KT (6-furanaminopurine), 0.1 mg 2,4-D (2,4-dichlorophenoxyacetic acid), 30 g sucrose, 6 g agar per liter of MS basic medium ;

[0024] Each li...

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Abstract

The present invention relates to tissue culture method of aquatic plant. The fast propagation process of Potarnogeton lucens includes the following steps: 1. taking the underground stem tip as the culture; 2. washing the culture; 3. inoculating to inducing culture medium to culture; 4. transferring to differentiating and proliferating culture medium to culture; 5. further culturing the separated buds in the differentiating and proliferating culture medium to obtain great amount of Potarnogeton lucens buds, or transferring the Potarnogeton lucens buds in rooting culture medium to induce rooting and to obtain Potarnogeton lucens seedling. The present invention has optimized recipes of inducing culture medium, differentiating and proliferating culture medium and rooting culture medium, and can propagate great amount of Potarnogeton lucens seedlings used for water body vegetation.

Description

Technical field [0001] The invention relates to a method for tissue culture of aquatic plants, in particular to a method for rapid propagation of Potamogton lucens Linn, which belongs to the application of biotechnology in the production of seedlings in water body vegetation restoration and waterscape layout. Background technique [0002] Potentilla spp. belongs to the genus Potentillaceae. Its leaves are yellow-green or bright green for viewing and are more resistant to pollution. It is an important plant resource in the biological food chain and the main aquatic organism in the restoration of water vegetation. plant. The leaves of Potentilla glabra are oblong, ovate-elliptic to lanceolate-elliptic, sessile or short-stalked, sometimes up to 2 cm in length; the leaves are 2-18 cm long and 0.8-3.5 cm wide , Thin, sharp apex, often with 0.5-2 cm long awn-like tip, wedge-shaped base, shallow wavy edge, sparsely and slightly serrated; 5-9 leaf veins, lifelike in clear water. [0003]...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01G7/00
Inventor 赵家荣刘艳玲徐立铭
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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