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Peony tissue culture and rapid propagation method

A technology of tissue culture and peony, applied in the field of plant tissue culture, can solve the problems that hinder the development of commercialization, have not yet established a complete rapid propagation system, and difficulty in rooting, and achieve the effect of promoting the process of commercialization

Inactive Publication Date: 2015-01-28
杨业容
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, peony mainly relies on division propagation for seedling production, which has problems such as long reproduction cycle, low efficiency, and low reproduction coefficient, and a sound industrial production mechanism has not yet been formed.
Due to problems such as serious pollution, high browning mortality, and difficulty in rooting, the development of tissue culture rapid propagation technology of peony has been slow, and a complete rapid propagation system has not been established, and the factory production of peony seedlings cannot be carried out, which seriously hinders its commercial development. process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Inducing adventitious buds: select vigorous and plump underground buds with a diameter of 2 to 3 cm, wash them with clean water, peel off the outer layer of scales, use a brush to dip in the washing liquid to clean each underground bud, and place them in the washing liquid Soak in water for 20 minutes, rinse with tap water for 45 minutes, soak in 75% ethanol in an ultra-clean workbench for 10 seconds, rinse with sterile water for 5 minutes each time, then disinfect with 0.1% mercuric chloride solution for 10 minutes, and then use bacteria-cleaning water Rinse 5 times, 3 minutes each time. Blot the water droplets on the surface with sterile filter paper and inoculate them on the induction medium to start the culture. The washing liquid is 0.02% potassium permanganate solution, and the proliferation medium is 1 / 2MS (Mg 2+ double)+2mg / L 6-BA+0.1 / L NAA+0.5mg / LGA 3 +3.5% sucrose+0.5% agar+0.05% activated carbon, the pH value is 5.8.

[0020] (2) Proliferation culture ...

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Abstract

A peony tissue culture and rapid propagation method relates to Paeonialactiflora Pall seedlings which are rapidly propagated in short time through a tissue culture technical means to keep parent original excellent properties. The peony tissue culture and rapid propagation is realized by using peony subterraneous stems as explants through the stages of peony starting culture, cluster bud induction, cluster bud enrichment culture, test-tube plantlet rooting, test-tube plantlet domestication transplanting and the like. Therefore, the rapid propagation of the peony seedlings is realized under the conditions of manual control, massive test-tube plantlets are obtained, and the peony commercializing process is promoted.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapid propagation of peony through tissue culture. Background technique [0002] Peony ( Paeonia lactiflora Pall.) is a perennial herbaceous plant of the whole leaf subgroup of Paeoniaceae Paeoniaceae. It is a traditional Chinese flower with high ornamental value and is deeply loved by the masses. It is as famous as peony and is called "the queen of flowers", "Flower phase". In addition, peony plants are rich in paeoniflorin, which has activities such as relieving labor pains and curbing the growth of tumor cells, has high medicinal value, and has a broad market prospect. [0003] At present, peony mainly relies on division propagation for seedling production, which has problems such as long reproduction cycle, low efficiency, and low reproduction coefficient, and a sound industrial production mechanism has not yet been formed....

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨业容
Owner 杨业容
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