Method for obtaining excellent clone through fiber bamboo subterraneous stem in-situ multiple mutagenesis

Abstract

The invention discloses a method for obtaining an excellent clone through fiber bamboo subterraneous stem in-situ multiple mutagenesis. The method comprises the steps that a subterraneous stem of fiber bamboo is selected; an internal irradiation mutagenic agent is selected to be prepared into mutagenic agent solutions separately; a chemical mutagenic agent ethylmethane sulfonate is selected to beprepared into mutagenic agent solutions separately; a stainless steel continuous syringe is used for filling different segments of the selected subterraneous stem of the fiber bamboo with the internalirradiation mutagenic agent of different concentrations, and injection ports are sealed by non-sticking adhesive tapes; after 30-40 days, the stainless steel continuous syringe is used for filling the different segments of the selected subterraneous stem of the fiber bamboo with the chemical mutagenic agent solution, and the injection ports are sealed by non-sticking adhesive tapes; after earthing is performed for 60-90 days, different mutagenic buds on the segments, subjected to multiple treatment of the internal irradiation mutagenesis and mechanical mutagenesis, of the subterraneous stem are germinated into bamboo, and through screening and underground root dividing propagation, the clonal new germplasm or new species of the fiber bamboo with excellent traits.

Description

technical field [0001] The invention relates to a method for obtaining an excellent clone of bamboo for fiber, and belongs to the technical field of plant breeding. Background technique [0002] The pulp and paper industry is one of the important pillar industries of China and even the world economy. With the rapid development of my country's economy, the demand for paper will continue to increase rapidly. Due to the relative shortage of forest resources in our country and the continuous improvement of laws and policies related to forest protection series, as well as the relatively low recycling rate of waste paper in the paper industry chain, the gap in timber resources can only be made up by imports to a large extent, and the country must not Import lumber and wood pulp without spending huge sums of money. However, as a natural plant resource widely distributed in my country, bamboo has the characteristics of wide distribution, strong adaptability, fast growth, early matu...

AI Technical Summary

Problems solved by technology

The history and current status of bamboo research in China are relatively ahead of other countries in the world, but due to the complexity of the genetic background of bamboo and the particularity of biology, that is, the characteristics of bamboo plants such as uncertainty in flowering and death after flowering, it is difficult to pass Variety improvement by traditional breeding methods

Existing breeding of fine bamboo varieties for fiber is mainly asexual reproduction from natural mutation selection or introduction and domestication from different places, and the variety type is relatively single

In addition, the genetic transformation system of bamboo is still immature, and it is difficult to genetically improve it through genetic engineering.

So far, no modern biotechnology system has been established for the creation and selection of new bamboo germplasm resources for fiber