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Eurotium cristatum LS1 strain

A technology of Saccharomyces coronoides and strains, applied in the field of microorganisms, can solve problems such as environmental pollution, chemical fungicides are difficult to decompose, and plant pathogenic bacteria resistance

Active Publication Date: 2016-08-17
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemical fungicides are not easy to decompose, and long-term use will easily cause environmental pollution, cause plant pathogenic bacteria to develop drug resistance, and their pesticide residues will endanger human health

Method used

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  • Eurotium cristatum LS1 strain
  • Eurotium cristatum LS1 strain
  • Eurotium cristatum LS1 strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Fu brick tea made from tea leaves collected in Qianyang, Hunan Province has "flowering phenomenon". Pick the golden-yellow closed cysts from Fuzhuan brick tea and inoculate them on PDA medium, culture at a constant temperature of 28°C for 5 days, pick mycelia for streaking on the plate, and repeat the purification 3 times to obtain the strain LS1 of the present invention.

Embodiment 2

[0015] The preparation of spore suspension: prepare the spore suspension of bacterial strain LS1 of the present invention with prior art, concentration is 3 * 10 7 pc / mL, for use.

[0016] Preparation of fermentation broth: the absorption concentration is 3×10 7 Inoculate 1% of the above-mentioned spore suspension per mL onto Pueraria root fermentation medium (2.5 g of Pueraria root and 10 mL of water), and culture at a constant temperature of 28°C. After 7 days, use 95% mass concentration of ethanol to ultrasonically extract the fermented product, take the supernatant, and volatilize it to a constant weight by a rotary evaporator to obtain a fermented product, which is diluted with distilled water to the fermented product to a mass concentration of 0.1 mg / mL.

[0017] Preparation of the unfermented liquid: pueraria puerariae was pulverized into powder, then diluted to 25 mL with distilled water, and the mass concentration of the unfermented liquid was 0.1 mg / mL.

Embodiment 3

[0019] Example 3 Inhibition to pathogenic fungi

[0020] The pathogenic fungi tested: Colletotrichum, Alternaria sonali, Sclerotinia sclerotiorum, and Alternaria brassicae were provided by the Microbiology Laboratory of the School of Food Science and Technology, Hunan Agricultural University.

[0021] Chemical bactericide hymexazol: purchased from Zhengzhou Yukang Biotechnology Company, with an active ingredient content of 80.1%; hymexazol was diluted 1000 times with sterile water and used as a positive control.

[0022] Treatment of treatment groups (Puderaria fermented liquid, Pueraria unfermented liquid) and the positive control group: adopt plate confrontation method, first pour 5mL PDA into a petri dish with a diameter of 90mm, add 2mL treatment group or hymexazol solution after solidification, shake After mixing well, add 15mL PDA. 2 mL of sterile water was added to the culture medium of the blank control group. After solidification, insert 3 mm of pathogenic bacteria ...

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Abstract

An eurotium cristatum LS1 strain is classified and named as EurotiumCristatum LS1 and preserved in China Center for Type Culture Collection on June 17, 2015, and the preservation number is CCTCC NO:M 2015379. The strain LS1 can grow on kudzuvine roots and conduct bioconversion, when the strain LS1 is used for fermenting kudzuvine roots, an obtained fermenting product has stronger inhibiting effects on cowpea anthracnose, tomato early blight, sclerotinia sclerotioru and Chinese cabbage leaf spots, and the inhibiting effects on five putrefactive bacteria of staphylococcus aureus, bacillus cereus, escherichia coli, bacillus subtilis and salmonella are all improved than those before fermentation is conducted. It is shown that a product obtained by fermenting kudzuvine roots through the strain LS1 has broad-spectrum antibacterial activity, can inhibit pathogenic fungi and the putrefactive bacteria to some extent, is expected to be developed into biopesticides to prevent and control crop diseases and is expected to serve as a food sterilizing agent to be used for food preservation.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a strain of Eurotium Crisstatum LS1. Background technique [0002] Food is susceptible to microbial contamination in raw material production, post-harvest, production, packaging, storage, transportation and sales. Among them, chemical fungicides have been widely used due to their advantages of low cost, simple operation and obvious effect. However, chemical fungicides are not easy to decompose, and long-term use will easily cause environmental pollution, cause plant pathogenic bacteria to develop drug resistance, and their pesticide residues will endanger human health. Due to the inherent disadvantages and irrational use of chemical fungicides, people have recognized the potential hazards of chemical fungicides, and began to pay attention to the development of broad-spectrum, efficient, safe and cheap new natural fungicides. Contents of the invention [0003] The technical problem...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P1/00A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 易有金王璐夏菠曹熙胡谢欣
Owner HUNAN AGRICULTURAL UNIV
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