A method for cultivating Bacillus amyloliquefaciens and its application

A technology for dissolving amyloid spores and bacilli, applied in the field of microorganisms, can solve problems such as affecting physical health, chemical residues, etc., and achieve the effects of simple use method, low cost, and reduced production cost

Inactive Publication Date: 2018-02-09
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] Aiming at the deficiencies of the prior art, the present invention provides a method for cultivating Bacillus amyloliquefaciens and its application, which can solve the problem of chemical residues produced by chemical agents for fresh fruits and vegetables, thereby affecting the health of the body

Method used

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  • A method for cultivating Bacillus amyloliquefaciens and its application
  • A method for cultivating Bacillus amyloliquefaciens and its application
  • A method for cultivating Bacillus amyloliquefaciens and its application

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Embodiment

[0041] 1 Isolation and purification of antagonistic strains

[0042] The bee grain was cultured with beef extract peptone liquid medium and potato glucose liquid culture respectively based on 28-37°C for 24-48 hours, and the dilution plate method was used to separate microorganisms, and the bacteria and fungi were separated using beef extract peptone liquid medium ( NA) and potato dextrose liquid medium (PDA) plates, respectively placed in 30 ~ 37 ℃ and 25 ~ 30 ℃ incubator culture 2 ~ 3d. According to the characteristics of the colony's shape, color, transparency, etc., single colonies with obvious morphological differences were picked for streaking and purification, and the slopes were stored at 4°C for later use. A total of 56 bacterial strains were obtained.

[0043] 2 Screening of antagonistic strains

[0044] 2.1 Fermentation broth preparation

[0045]Inoculate the bacterial strain to be screened in potato glucose liquid medium, and ferment and culture in a shake flask...

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Abstract

The invention discloses a method for cultivating a strain of Bacillus amyloliquefaciens and its application, belonging to the technical field of microbes. A strain of Bacillus amyloliquefaciens PC2 has a preservation number of CCTCC NO: M2015435. Beneficial effects of the present invention: the fermented liquid of Bacillus amyloliquefaciens strain provided by the present invention has broad-spectrum antibacterial activity, can effectively control post-harvest tomato fruit rot pathogen, is for post-harvest storage and preservation of tomato, reduces post-harvest loss, and improves Product quality and safety provide an effective, non-toxic, non-residue, and environmentally friendly new approach to biological control. The bacterial strain fermentation medium of the present invention has simple requirements, low cost, simple cultivation method, simple preparation process of the involved fresh-keeping agent, simple use method, and is suitable for popularization and application in production.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for cultivating a strain of Bacillus amyloliquefaciens and its application. Background technique [0002] Fruits and vegetables are important non-staple foods in people's lives, the second largest category of agricultural products after grain, and important raw materials for the food industry. Tomato is an important vegetable variety. There are many kinds of tomato postharvest storage diseases. Among them, Fusarium tomato fruit rot is a postharvest pathogenic fungus discovered in recent years. It mainly infects mature fruits and causes fruit soft rot. greater economic loss. [0003] my country's fruit and vegetable storage and preservation technology is relatively backward compared with developed countries, and the main method is to spray chemically synthesized fungicides for fruit and vegetable preservation. Long-term use will not only produce drug ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/07
CPCA23B7/155C12P1/04C12N1/205C12R2001/07
Inventor 申光辉冯孟张志清秦文陈安均刘书亮黎杉珊林德荣陈姝娟何利
Owner SICHUAN AGRI UNIV
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