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Yeast strain for producing biosurfactant and application thereof

A yeast strain and yeast technology, applied in the field of yeast and its application, can solve the problems of unstable emulsions and other problems, and achieve the effects of not easy to mutate, fast growth, and simple cultivation methods

Active Publication Date: 2012-11-07
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biosurfactants are small molecules with low molecular weight, and the emulsions they form often cannot exist stably

Method used

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  • Yeast strain for producing biosurfactant and application thereof
  • Yeast strain for producing biosurfactant and application thereof
  • Yeast strain for producing biosurfactant and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Isolation and purification of embodiment 1 yeast strain

[0053] 1. Culture medium preparation

[0054] (1) Basic culture medium:

[0055] (NH 4 ) 2 SO 4 1g, K 2 HPO 4 0.8g, KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.1g, FeSO 4 ·7H 2 O 5mg, 1mL filter-sterilized vitamin solution, 1,000mL purified water, pH 5-6, medium sterilized at 121°C for 20 minutes.

[0056] The composition of the vitamin solution is as follows: niacin 100mg, vitamin B1 100mg, biotin 5mg, p-aminobenzoic acid 50mg, vitamin B12 1mg, calcium pantothenate 50mg, vitamin B650mg, vitamin M 50mg, 3Na EDTA 200mg, diluted with sterilized purified water to 100mL.

[0057] (2) enrichment medium:

[0058] Add 0.2% phenanthrene solution to the basic medium, adjust the pH value to 5-6, and sterilize the medium at a constant temperature of 121° C. for 20 minutes; the composition of the phenanthrene solution is: 1 g phenanthrene, 1 L n-hexane.

[0059] (3) Separation and purification mediu...

Embodiment 2

[0083] The microbiological characteristic research of embodiment 2 yeast strains

[0084] 1. Colony morphology observation

[0085] Streak-inoculate the purified cultured yeast strains on YPD solid medium, culture at 27°C until colonies grow, and observe the colony morphology. The results are as follows:

[0086] The colony characteristics of the strain are: the diameter of the colony cultured on the YPD plate for 2 days is 5-6mm, the colony is round, the surface is rough, the edges are irregular, prominent, and pink.

[0087] 2. Observation of cell morphology

[0088] The strain was inoculated in YPD medium and cultured at 27°C and 150rpm for 3 days, and the cell morphology of the strain in the culture medium was observed with a microscope. The observation results were as follows: figure 1 As shown, the cells of yeast are elliptical, the major axis of mature cells is 7-8um, and the minor axis is 3-4um.

[0089] 3. 26S rDNA gene sequence determination

[0090] Inoculate ye...

Embodiment 3

[0094] The cultivation of embodiment 3 salmon color locking throwing yeast bacterial strain S.salmonicolor AH3

[0095] 1) Prepare the inoculation mother solution of yeast strain S.salmonicolor AH3

[0096] Take the fresh slant strain of yeast strain S.salmonicolor AH3 and inoculate it in naphthalene medium. Naphthalene is the only carbon source for the growth of yeast strain S.salmonicolor AH3. Cultivate at 27°C and 150rpm, and measure the OD of the culture medium 600 value until OD 600 When the value reaches 0.5, the inoculation mother solution of S.salmonicolor AH3 bacteria is obtained, wherein, one inoculation loop of S.salmonicolor AH3 bacteria is inoculated in every 100ml of naphthalene medium.

[0097] 2) Cultivation in hexadecane medium

[0098] The inoculation mother solution of bacterial strain S.salmonicolor AH3CGMCC No.4814 is inoculated in hexadecane medium, wherein, the inoculum size is 1% (v / v), promptly the ratio of the volume of inoculation mother solution a...

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Abstract

The invention discloses a yeast strain Sporidiobolus salmonicolor AH3 for producing a biosurfactant. The microbial collection number of the yeast strain is CGMCC No. 4814. The yeast strain provided by the invention can grow with C10-C24 alkane and / or polycyclic aromatic hydrocarbons as the carbon source, and the biosurfactant is generated. When the yeast strain is cultured with alkane and polycyclic aromatic hydrocarbons as the carbon source, emulsifying ability of the generated biosurfactant is enhanced. The yeast strain Sporidiobolus salmonicolor AH3 CGMCC No.4814 has good effects of degrading and emulsifying hydrophobic organic matters, and has a good application prospect in treatment of petroleum produced wastewater.

Description

technical field [0001] The invention relates to a yeast in the field of environmental biology and its application, in particular to a yeast producing a surfactant and its application in oil production wastewater treatment, which belongs to the field of yeast strains. Background technique [0002] Oily wastewater mainly comes from petroleum, petrochemical, steel, coking, gas generation stations, mechanical processing and other industrial sectors. The oily substances contained in it include natural petroleum, petroleum products, tar and its fractions, and edible animal and vegetable oils and fats class etc. If the oily wastewater produced in the production process of these industrial sectors is not recycled, it will cause a great waste of resources; if it is directly discharged into rivers, lakes or bays without treatment, it will pollute the water body, and the hydrophobic organic matter in the oily wastewater will be in the environment. An oil film is formed on the surface ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P1/02A62D3/02C02F3/34C12R1/645C02F101/30A62D101/20
Inventor 张昱邓艳芹杨敏黑山姆吕文洲
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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