43 results about "Polyinosinic Acids" patented technology

The present invention provides a polynucleotide adjuvant (PICKCa) composition and methods of use in eliciting an immune response, in particular a mucosal immune response. The polynucleotide adjuvant comprises of a polyriboinosinic-polyribocytidylic acid (PIC), at least one antibiotic and at least one positive ion. The present invention also provides an immunogenic composition comprising the polynucleotide adjuvant composition together with other immunogenic compositions such as an antigen (e.g., as in a vaccine) selected from viral, bacterial, fungal, parasitic and / or cancer antigens. The present invention further contemplates methods of use of such adjuvant compositions, particularly in eliciting an immune response, in particular a mucosal immune response to an antigenic compound.

The present invention provides a polynucleotide adjuvant (PICKCa) composition and methods of use in eliciting an immune response, in particular a mucosal immune response. The polynucleotide adjuvant comprises of a polyriboinosinic-polyri-bocytidylic acid (PIC), at least one antibiotic and at least one positive ion. The present invention also provides an immunogenic composition comprising the polynucleotide adjuvant composition together with other immunogenic compositions such as an antigen (e.g., as in a vaccine). The present invention further contemplates methods of use of such adjuvant compositions, particularly in eliciting an immune response, in particular a mucosal immune response to an antigenic compound.

The present invention provides a polynucleotide adjuvant composition and methods of use in eliciting an immune response. The present invention also provides an immunogenic composition comprising the polynucleotide adjuvant composition together with an antigen (e.g., as in a vaccine). The adjuvant compositions of the invention have particular physical properties (e.g., molecular weight, concentration, and pH) which address the need for a safe adjuvant for eliciting an enhanced immune response. The present invention further contemplates methods of use of such adjuvant compositions, particularly in eliciting an immune response to an antigenic compound.

The invention relates to the field of immunology, and particularly discloses a mucosal immunity preparation capable of resisting infection and tumors. The effective component of the preparation is a mucosal immunity substance which is formed by combining polyinosinic acid-polycytidylic acid, a non-antibiotic amino compound, metal positive ions, and PEG and / or PEI and / or PLGA and / or positive ion polymers through hydrogen bonds and other chemical bonds. The preparation is a spray or an aerosol in dosage form, but preferentially, the preparation does not contain ingredients utilized by common sprays or ingredients utilized by aerosols, and propellants. The mucosal immunity preparation can achieve the slow-release function partially or wholly, the half-life period of the mucosal immunity preparation is prolonged, the availability and effectiveness of drugs are improved, and the compliance of a patient is improved. The mucosal immunity preparation can promote mucosal immunity of the human body by means of the mucosal immunity, and then promote activation and proliferation, including whole-body nonspecific immunity, humoral immunity and cellular immunity, of all immune cells instead of only acting on the local part of a disease wound, and thus the preventing and treating effects of resisting infection and tumors are achieved.

The invention provides a method for synthesizing silver nanoclusters by the aid of single-stranded DNA (deoxyribonucleic acid) used as a template, and belongs to the technical field of nano materials. By the method, problems of complicated production and excessively high cost of nano-silver for detecting mercury ions at present are solved. The method for synthesizing the silver nanoclusters by the aid of the single-stranded DNA used as the template is characterized in that the single-stranded DNA containing cytosine and guanine is used as the template and is mixed with Ag<+> solution to obtain a mixture, reducing agents are added into the mixture, and the fluorescent silver nanoclusters can be acquired after reduction reaction is performed on the mixture with the reducing agents. The method has the advantages that polyinosinic acid (PI) and polycytidylic acid (PC) which are commercially available are used as the template in the simple, low-cost, environment-friendly and reliable method for synthesizing the silver nanoclusters; fluorescence is utilized, and fluorescent silver nanocrystals are synthesized from the polyinosinic acid, the polycytidylic acid and the like without special design or preprocessing; and the novel silver nanoclusters are excellent in selectivity and sensitivity, can be applied to detecting Hg<2+>, and are feasible and have a future development prospect in the aspect of monitoring pollutants in water.

The invention provides a preparation method of refined poly zic and application, which are characterized in that polyinosinic acid and poly zic acid are purified via a chemical method, are merged into poly zic liquor after being refined respectively, and the poly zic liquor is ultra-filtered, concentrated and added with auxiliaries, so that the refined poly zic is prepared. The refined poly zic can effectively increase an anticancer effect.

The invention discloses a foot-and-mouth disease genetic engineering mixed epitope vaccine and a preparation method thereof. The vaccine consists of the following four parts: a serial B cell epitope recombinant protein BI consisting of main neutralizing epitops of O-type foot-and-mouth disease viruses in Cathay, Transasia and Mya 98 pedigrees with a gene sequence of SEQ ID NO:1 and an amino acid sequence of SEQ ID NO:2, a T-cell epitope recombinant protein TI consisting of serial connection of universal T-cell epitope and a plurality of foot-and-mouth disease virus specific T-cell epitopes with a gene sequence of SEQ ID NO:3 and an amino acid sequence of SEQ ID NO:4, Toll-like receptor 3 agonist-polyinosinic acid-polycytidysic acid and / or Toll-like receptor7 / 8 agonist-R848 serving as immunopotentiator, and 201 oil adjuvant. When being used for immunizing a pig, the BI and TI mixed epitope vaccine prepared by utilizing the method can produce a protective immunization effect the same as or better than that of an inactivated influenza virus Vaccines, and has a cross protection effect to viruses of the three pedigrees, so that the vaccine is a novel immune-enhanced O-type foot-and-mouth genetic engineering mixed epitope vaccine.

The invention provides a preparation method of polyinosinic-polycytidylic acid dry powder. The method comprises the following steps: (1) respectively dissolving polyinosinic acid and polycytidysic acid in a phosphate buffer solution, mixing the solution, adding a stabilizer, uniformly mixing the stabilizer and the solution, and performing heat preservation on the mixture at the temperature of 40-100DEG C; (2) naturally cooling the reaction liquid obtained in the step (1), mixing the reaction liquid with an organic solvent, standing for precipitating, and drying the precipitate, thereby obtaining the polyinosinic-polycytidylic acid dry powder. The preparation method has the beneficial effects that the prepared polyinosinic-polycytidylic acid dry powder has a complete double-helix structural features and physiological activity, the quality and the stability are better than those of the polyinosinic-polycytidylic acid at a solution state, a stronger enzymolysis-resisting performance can be achieved compared with the bare polyinosinic-polycytidylic acid after entering a living body, so that the curative effect can be enhanced. The polyinosinic-polycytidylic acid is purified in the process for making the polyinosinic-polycytidylic acid solution into the dry powder, a plurality of small-molecular-weight substances and impurities can be removed, the toxic and side effects can be reduced, and the quality of the polyinosinic-polycytidylic acid can be improved.

The present invention relates to a kind of composite adjuvant composition and the vaccine composition containing this adjuvant, composite adjuvant composition contains oil-in-water emulsion, polyinosinic acid-polycytidylic acid or derivatives thereof and DDA, can significantly improve Cellular immunity to antigens.