Method for efficiently producing DC-CIK cells through induction of polyinosinic: polycytidylic acid copolymer
A technology of DC-CIK and polymyocytes, applied in animal cells, vertebrate cells, cell culture active agents, etc., can solve the problems of ineffective identification and killing of tumor/cancer cells, low immune system, etc., and achieve growth The possibility of great cure, the effect of improving the quality of life and prolonging the survival time
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038] Embodiment 1, the selection of the optimal concentration of polymyocytes
[0039] Material:
[0040] 1. Polymyocytes (Place of origin: Shanghai Yuanye Biotechnology Co., Ltd.; Cas: 24939-03-5)
[0041] 2.Hyclone RPMI 1640
[0042] 3. Peripheral anticoagulation of healthy volunteers
[0043] 4. Conditioned medium: containing IL-2 100000IU / ml, anti-human CD3 humanized chimeric antibody 1000μg / ml, IL-1α
[0044] 10000ng / ml, GMCSF10000ng / ml, gamma interferon 10000ng / ml.
[0045] 5. CCK8 detection kit (Beiren Chemical Technology (Beijing) Co., Ltd., model: CK04)
[0046] 6. Others: plate microplate reader (Shenzhen Redu Life Science Co., Ltd., model: RT-6100), carbon dioxide incubator (Thermo SCIENTIFIC, model: 3111), clean bench (Thermo SCIENTIFIC, model: 1379), etc.
[0047] Prepare:
[0048] 1. Preparation of DC-CIK medium: Take the plasma-containing medium in "method step 2" respectively, and add 500 μl of DC-CIK conditioned medium according to the ratio of DC-CIK ...
Embodiment 2
[0067] Example 2 Effects of Polymyocytes on the Proliferation of DC-CIK
[0068] Material:
[0069] 1. Polymyocytes (Shanghai Yuanye Biotechnology Co., Ltd.; Cas: 24939-03-5)
[0070] 2.Hyclone RPMI 1640
[0071] 3. Peripheral anticoagulation of healthy volunteers
[0072] 4. Conditioned medium: containing IL-2 100000IU / ml, anti-human CD3 humanized chimeric antibody 1000μg / ml, IL-1α10000ng / ml, GMCSF10000ng / ml, gamma interferon 10000ng / ml.
[0073] 5. Plate microplate reader (Shenzhen Redu Life Science Co., Ltd., model: RT-6100), carbon dioxide incubator (Thermo SCIENTIFIC, model: 3111), clean bench (Thermo SCIENTIFIC, model: 1379), etc.
[0074] Prepare:
[0075] 1. Production of DC-CIK medium for the control group: Take 250ml of the HycloneRPMI 1640 medium containing 2% autologous plasma in "Method Step 2" and add 2.5ml of DC-CIK conditioned medium to form Control DC-CIK- Hyclone RPMI 1640 medium.
[0076] 2. Production of DC-CIK medium in the experimental group: Take 2...
Embodiment 3
[0092] Example 3 Identification of highly efficient DC-CIK phenotype induced by polymyocytes
[0093] Material:
[0094] 1. Polymyocytes (Shanghai Yuanye Biotechnology Co., Ltd.; Cas: 24939-03-5)
[0095] 2.Hyclone RPMI 1640
[0096] 3. Peripheral anticoagulation of healthy volunteers
[0097] 4. Conditioned medium: containing IL-2 100000IU / ml, anti-human CD3 humanized chimeric antibody 1000μg / ml, IL-1α10000ng / ml, GMCSF10000ng / ml, gamma interferon 10000ng / ml.
[0098] 5. Flow cytometry antibody CD3: BV510, CD4: BUV496, CD8: BV786
[0099] 6. Others: flow cytometer (BD, model: FACSymphony), carbon dioxide incubator (ThermoSCIENTIFIC, model: 3111), clean bench (Thermo SCIENTIFIC, model: 1379), etc.
[0100] Prepare:
[0101] 1. Production of DC-CIK medium for the control group: Take 250ml of Hyclone RPMI1640 medium containing 2% autologous plasma in "method step 2", add 2.5ml of DC-CIK conditioned medium to form DC-CIK conditioned medium, namely into ControlDC-CIK-Hyclone ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com