The invention discloses a photoacoustic imaging surgical navigation platform for guiding tumor excision, which comprises photoacoustic imaging equipment, and the photoacoustic imaging equipment comprises a main body, a probe and an image display. The invention discloses the photoacoustic imaging operation navigation platform for guiding tumor excision, and high-frame-rate photoacoustic images andultrasonic images can be provided; deep tissue tumors are visualized in real time; the position of the tumor can be positioned and a tumor boundary is accurately drawn; meanwhile, an aseptic scalpel can be visually improved through photoacoustic imaging; simultaneous development of the tumor and the scalpel under photoacoustic imaging is realized, and the focus can be thoroughly excised by directly viewing the tumor boundary without missing or mistaken cutting, the technology can be used for tumor positioning, boundary defining and accurate excision in the operation process, accurate intraoperative guidance is provided for tumor surgeons, the incompleteness of intraoperative tumor excision is reduced, the thorough excision rate is increased, normal tissue is reserved to the maximum extent,tumor recurrence is reduced, and the survival rate is increased.