40 results about "Polyinosinic:polycytidylic acid" patented technology

The invention discloses application of a polyinosinic-polycytidylic acid (poly I:C) combined dimo-thylidioctyl ammonium bromide (DDA) mixed adjuvant to the preparation of tuberculosis subunit vaccines. The invention has the advantages that: the mixed adjuvant DDA / Poly I:C can enhance the secretion level of antigenic specificity interferon-gamma (IFN-gamma) of fusion protein Ag85B-MPT64190-198-Mtb8.4 (AMM) effectively and relieve pathological damage caused by H37Rv infection, and is an effective adjuvant of vaccines for preventing tuberculosis.

The invention provides a preparation method of polyinosinic-polycytidylic acid dry powder. The method comprises the following steps: (1) respectively dissolving polyinosinic acid and polycytidysic acid in a phosphate buffer solution, mixing the solution, adding a stabilizer, uniformly mixing the stabilizer and the solution, and performing heat preservation on the mixture at the temperature of 40-100DEG C; (2) naturally cooling the reaction liquid obtained in the step (1), mixing the reaction liquid with an organic solvent, standing for precipitating, and drying the precipitate, thereby obtaining the polyinosinic-polycytidylic acid dry powder. The preparation method has the beneficial effects that the prepared polyinosinic-polycytidylic acid dry powder has a complete double-helix structural features and physiological activity, the quality and the stability are better than those of the polyinosinic-polycytidylic acid at a solution state, a stronger enzymolysis-resisting performance can be achieved compared with the bare polyinosinic-polycytidylic acid after entering a living body, so that the curative effect can be enhanced. The polyinosinic-polycytidylic acid is purified in the process for making the polyinosinic-polycytidylic acid solution into the dry powder, a plurality of small-molecular-weight substances and impurities can be removed, the toxic and side effects can be reduced, and the quality of the polyinosinic-polycytidylic acid can be improved.

The purpose of the present invention is to provide a riemerella anatipestifer bacterial ghost vaccine loaded with polyinosinic acid-polycytidylic acid having interferon inducing activity and added with chitosan oligosaccharide as an adjuvant. According to the technical scheme, a temperature control expression vector is constructed and is electrotransformed into riemerella anatipestifer protoplast, positive clones are screened and then are subjected to bacterial amplification culture at a temperature of 28 DEG C, lysis gene expression is induced at a temperature of 42 DEG C, polylysine is added to continuously act when the OD600 is no longer be reduced so as to completely lyze the live bacteria, centrifugation is performed to separate the bacteria, the dried bacteria and a 6 mg / mL Poly I:C solution according to an equal ratio so as to make the Poly I:C be embedded into the bacterial ghosts, a 2% chitosan oligosaccharide aqueous solution is added, stirring is performed to form a uniform mixture, sub-packaging is performed into penicillin bottles, and freeze-drying is performed so as to obtain the novel riemerella anatipestifer bacterial ghost vaccine. According to the present invention, the prepared riemerella anatipestifer bacterial ghost vaccine is immunized through drinking water, such that the local immunity on the respiratory tract mucous membrane and the digestive tract mucous membrane can be irritated, the humoral immunity can be stimulated, and the protection effect on the human body is not different from the injection immunity.

The invention discloses fluorescence quantitative reference genes for a crassostrea gigas Poly (I:C) stress experiment as well as primers and applications of the fluorescence quantitative reference genes, relating to the field of molecular biology and particularly relating to the screening of fluorescence quantitative reference genes for a crassostrea gigas polyinosinic-polycytidylic acid stress experiment. The screened beta-actin, GAPDH and RS18 are three reference genes which are most stable in expression in gigas blood cells in the Poly (I:C) stress experiment. The expression stabilities of the three reference genes are superior to the expression stabilities of the other seven reference genes (including common 28S, rRNA and Elongation factor-1alpha) in the Poly (I:C) stress experiment, so that the three reference genes can be simultaneously used as the fluorescence quantitative reference genes to provide powerful support for obtaining precise quantitative data for functional gene expression in the crassostrea gigas Poly (I:C) stress experiment.

The invention provides a preparation method of poultry specific transfer factors, relating to the field of medicines, in particular to a preparation method of a poultry specific transfer factor. The preparation method comprises the steps of: with pig spleen or peripheral blood as a raw material, preparing a lymphocyte monolayer, culturing lymphocyte in vitro, then inducing and culturing by using polyinosinic polycytidylic acid and infectious bronchitis virus, and producing a large quantity of infectious bronchitis virus resistant specific transfer factors in vitro. The infectious bronchitis virus resistant specific transfer factors prepared in vitro by using the method are used for treating the infectious bronchitis and improving poultry immunity.

Natural Killer (NK) cells are obtained by administering a Toll-like receptor ligand such as polyinosinic-polycytidylic acid into the peritoneal cavity of an animal to which lactoferrin has been administered to proliferate NK cells in the peritoneal cavity and collecting NK cells from the peritoneal cavity.