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Preparation method of polyinosinic-polycytidylic acid dry powder

A technology of double-chain polymyocytes and dry powder, which is applied in the field of preparation of double-chain polymyocyte dry powder, can solve the problems of increasing the difficulty of monitoring, increasing the steps of polymerization reaction, and increasing the complexity of operation, so as to achieve increased stability and enhanced curative effect , easy-to-operate effects

Active Publication Date: 2014-02-26
美亚药业海安有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its shortcoming is that, first of all, adding a polymerization reaction step to the preparation production line not only increases the complexity of the operation, but also increases the difficulty of monitoring

Method used

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  • Preparation method of polyinosinic-polycytidylic acid dry powder
  • Preparation method of polyinosinic-polycytidylic acid dry powder
  • Preparation method of polyinosinic-polycytidylic acid dry powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Weigh 5.2g of polyinosinic acid and 5.5g of polycytidylic acid and dissolve them in 100mL of phosphate buffer (pH6.4) and mix them, add 10.7g of chitosan oligosaccharide and mix evenly, keep warm at 60°C for 60min, cool naturally, and then Precipitate with 400mL of ethanol for 2 hours to obtain the polyinocytic precipitate, and dry at 20°C to obtain the double-chain polyinogenic dry powder (the content of the double-chain polyinocytic is 52.8%).

[0035] The color enhancement effect of the mixed solution and dry powder at 248nm was detected respectively, and the results are as follows: the color enhancement effect of the solution is 56.4%, and the color enhancement effect of the dry powder is 66.4%.

Embodiment 2

[0037] Weigh 3.5g of polyinosinic acid and 5.2g of polycytidylic acid (1:1.5) each dissolved in 100mL phosphate buffer (pH7.2) and mix them, add 8.7g of kanamycin and mix well, keep warm at 80°C for 60min , after natural cooling, then precipitated with 400mL of ethanol for 12 hours to obtain a polysarcoma precipitate, and dried at 35°C to obtain a double-stranded polysarcoma dry powder (the content of which is 51.2%).

[0038] The color enhancement effect of the mixed solution and dry powder at 248nm was detected respectively, and the results are as follows: the color enhancement effect of the solution is 55.3%, and the color enhancement effect of the dry powder is 57.9%.

Embodiment 3

[0040] Weigh 5.4g of polyinosinic acid and 5.1g of polycytidylic acid and dissolve them in 100mL of phosphate buffer (pH7.5), mix them, add 10.5g of kanamycin and 1.5g of calcium chloride, mix well, and keep warm at 60°C After cooling for 60 minutes, 400 mL of methanol was used to precipitate for 24 hours to obtain a polysarcoma precipitate, which was dried at 60°C to obtain a double-stranded polysarcoma dry powder (the content of which was 45.8%).

[0041] The color enhancement effect of the mixed solution and dry powder at 248nm was detected respectively, and the results are as follows: the color enhancement effect of the solution is 65.5%, and the color enhancement effect of the dry powder is 68.6%.

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Abstract

The invention provides a preparation method of polyinosinic-polycytidylic acid dry powder. The method comprises the following steps: (1) respectively dissolving polyinosinic acid and polycytidysic acid in a phosphate buffer solution, mixing the solution, adding a stabilizer, uniformly mixing the stabilizer and the solution, and performing heat preservation on the mixture at the temperature of 40-100DEG C; (2) naturally cooling the reaction liquid obtained in the step (1), mixing the reaction liquid with an organic solvent, standing for precipitating, and drying the precipitate, thereby obtaining the polyinosinic-polycytidylic acid dry powder. The preparation method has the beneficial effects that the prepared polyinosinic-polycytidylic acid dry powder has a complete double-helix structural features and physiological activity, the quality and the stability are better than those of the polyinosinic-polycytidylic acid at a solution state, a stronger enzymolysis-resisting performance can be achieved compared with the bare polyinosinic-polycytidylic acid after entering a living body, so that the curative effect can be enhanced. The polyinosinic-polycytidylic acid is purified in the process for making the polyinosinic-polycytidylic acid solution into the dry powder, a plurality of small-molecular-weight substances and impurities can be removed, the toxic and side effects can be reduced, and the quality of the polyinosinic-polycytidylic acid can be improved.

Description

technical field [0001] The invention relates to a preparation method of double-chain polymuscular cell dry powder. Background technique [0002] Double-stranded polymyocyte is a kind of artificially synthesized double-stranded ribonucleic acid, which can induce the production of interferon and has broad-spectrum antiviral effect; in addition, it can also regulate the body's immune function, enhance the body's non-specific immune function and some specific immune functions , to achieve anti-hepatic necrosis and anti-tumor effects. [0003] The application of double-strand polykinocytes in cosmetics can eliminate skin growths such as acne and flat warts. [0004] In veterinary medicine, double-strand polymyocytes are mainly used in the prevention and treatment of viral diseases in poultry and livestock. It is easy to use and widely used. It can be used with many drugs at the same time, and has synergistic or additive effects. This product has no species specificity, and can ...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K31/80A61K39/39A61K8/84A61K47/36A61K47/34A61K47/26A61K47/12A61K47/04A61P1/16A61P35/00A61P31/12A61P37/02A61P37/04A61P17/10A61P17/12
Inventor 傅得响卢凰姗王宏费丽燕郭苏
Owner 美亚药业海安有限公司
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