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SNP (Single Nucleotide Polymorphism) molecular marker capable of influencing quantity of pig red blood cells

A molecular marker, red blood cell technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of simple structure and single function of red blood cells, and achieve the effect of good specificity and high sensitivity

Active Publication Date: 2018-11-23
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, it has been believed that red blood cells have a simple structure and a single function, which is only to transport O 2 and CO 2 Tool of

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker capable of influencing quantity of pig red blood cells
  • SNP (Single Nucleotide Polymorphism) molecular marker capable of influencing quantity of pig red blood cells
  • SNP (Single Nucleotide Polymorphism) molecular marker capable of influencing quantity of pig red blood cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Genotyping Detection

[0025] (1) Using phenol extraction method to extract the ear tissue genomic DNA of Duroc×Erhualian F2 population

[0026]1) Grind the ear tissue of Duroc×Erhualian F2 generation pig population (provided by Guangdong Huanong Wens Animal Husbandry Co., Ltd.) in liquid nitrogen, add an equal volume of 1×SET (1mL), proteinase K (10ng / mL) to a final concentration of 200ug / mL, then add 10% sodium dodecyl sulfate (SDS) to a final concentration of 0.5%, shake well, and incubate overnight in a 55°C water bath for digestion.

[0027] 2) Add an equal volume of Tris-saturated phenol to the digested tissue sample, slowly invert the centrifuge tube for 15 minutes, centrifuge in a low-temperature refrigerated centrifuge at 4°C and 11,000 rpm for 10 minutes, and carefully transfer the supernatant to another centrifuge tube. Mark the corresponding mark.

[0028] 3) Add an equal volume of phenol / chloroform / isoamyl alcohol (volume ratio 25:24:1), slowl...

Embodiment 2

[0037] Example 2: Application of the molecular marker typing method of the present invention in correlation analysis of pig immune traits

[0038] (1) Association analysis between MARC0065518 molecular marker typing results and immune traits

[0039] The experimental pigs used for the detection and analysis of the association between genotype and immune traits were from the F2 generation population of Duroc × Erhualian hybrid (both conventional breeds) bred by Guangdong Huanong Wen's Animal Husbandry Co., Ltd. The DNA used for genotyping is extracted from the ear sample of the F2 generation of Duroc×Erhualian hybrid ("Duroc×Erhualian hybrid F2 generation" referred to as "pig" in the text of the manual and the table) is used for routine blood testing Blood for flow cytometric analysis was collected from 80-day-old pigs 4 hours after polymyocyte (PolyI:C) inoculation. The method based on fixed model and random model was adopted, and gender was not considered as a fixed effect, ...

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Abstract

The invention belongs to the field of screening of pig molecular markers and relates to an SNP (Single Nucleotide Polymorphism) molecular marker capable of influencing the quantity of red blood cellsof pigs. The molecular marker is especially correlated with an SNP molecular marker of a red blood cell quantity property of 80-day-old pigs and is cloned from a gene with a registration number of MARC0065518; a gene chip technology is used for carrying out typing screening on the gene to obtain the molecular marker which is correlated with the quantity of the red blood cells of the pigs infectedwith polyinosinic-polycytidylic acid, and a nucleotide sequence is shown as SEQ ID NO: 1; allelic mutation of one T / C occurs on a 101st basic group of the sequence; the SEQ ID NO: 1 sequence has polymorphism due to the mutation. When 101st basic nucleotide on the SEQ ID NO: 1 is C, the pigs have higher red blood cell quantity. The invention discloses a method for screening the molecular marker andapplication of correlation analysis of the molecular marker. The invention provides the novel SNP molecular marker for immunological properties of the pigs, especially marker-assisted selection of the red blood cell quantity.

Description

technical field [0001] The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to a SNP molecular marker that affects the number of red blood cells in pigs, especially related to the SNP molecular marker for the trait of red blood cell number in 80-day-old pigs. The molecular marker of the invention can be used for the prediction of the character of the number of red blood cells (RBC) of pigs. Background technique [0002] In recent years, with the rapid development of my country's animal husbandry, people's pure pursuit of high-yield performance in breeding practice has led to a continuous decrease in the resistance of livestock and poultry, and the increase in the degree of intensive breeding and changes in feeding conditions have also caused many new infectious diseases. Popular (Yan Yan, 2007). According to statistics, the annual economic loss of animal husbandry in developing countries due to diseases exceeds 30%, ev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘向东李信颉赵书红李新云南九红尹立林唐振双杜小勇朱猛进张杰
Owner HUAZHONG AGRI UNIV
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