This invention provides a combination of microRNAs for evaluating the physiological and / or
pathological condition of a subject, wherein the combination comprises all detectable microRNAs stably existing in the serum /
plasma of a subject; and a method for evaluating the physiological and / or
pathological condition of a subject, wherein the method includes determining all detectable microRNAs stably existing in the serum /
plasma of a subject; and a kit for evaluating the physiological and / or
pathological condition of a subject, wherein the kit contains the tools for determining all detectable microRNAs that stably existing in the serum /
plasma of a subject; and a
biochip for evaluating the physiological and / or pathological condition of a subject, wherein the
biochip contains the components for determining all detectable microRNAs stably existing in the serum / plasma of a subject. The aforementioned combination, method, kit and
biochip can be used for diagnosis as well as differentially diagnosis of diseases including various tumors; various acute / chronic infectious diseases, e.g. viral diseases such as viral influenza,
viral hepatitis, AIDS, SARS, bacterial diseases such as
tuberculosis,
bacterial pneumonia, and other acute / chronic infectious diseases caused by various pathogenic microorganisms; other acute / chronic diseases such as diseases of
respiratory system, diseases of
immune system, diseases of blood and hematopoietic
system, diseases of
circulatory system such as cardio-cerebrovascular diseases, metabolic diseases of
endocrine system, diseases of digestive
system, diseases of
nervous system, diseases of
urinary system, diseases of
reproductive system and diseases of locomotor
system, prediction of complications occurrence and malignant diseases relapse, evaluation of therapeutic effects, screening of pharmaceutical active ingredients, assessment of
drug efficacy as well as forensic
authentication and prohibited
drug inspection and the like, possessing a number of advantages such as extensive detection spectrum, high sensitivity, low cost, convenience for sampling, ease for sample preservation, etc. The said method can be widely used in work related to general survey of diseases and so on, improve the low-specificity and low-sensitivity caused by individual differences which single markers are difficult to overcome, significantly increasing the clinical
detection rate of diseases, all of which make it become an effective means for diagnosing diseases in an
early phase.