Foot-and-mouth disease genetic engineering mixed epitope vaccine and preparation method thereof

Abstract

The invention discloses a foot-and-mouth disease genetic engineering mixed epitope vaccine and a preparation method thereof. The vaccine consists of the following four parts: a serial B cell epitope recombinant protein BI consisting of main neutralizing epitops of O-type foot-and-mouth disease viruses in Cathay, Transasia and Mya 98 pedigrees with a gene sequence of SEQ ID NO:1 and an amino acid sequence of SEQ ID NO:2, a T-cell epitope recombinant protein TI consisting of serial connection of universal T-cell epitope and a plurality of foot-and-mouth disease virus specific T-cell epitopes with a gene sequence of SEQ ID NO:3 and an amino acid sequence of SEQ ID NO:4, Toll-like receptor 3 agonist-polyinosinic acid-polycytidysic acid and / or Toll-like receptor7 / 8 agonist-R848 serving as immunopotentiator, and 201 oil adjuvant. When being used for immunizing a pig, the BI and TI mixed epitope vaccine prepared by utilizing the method can produce a protective immunization effect the same as or better than that of an inactivated influenza virus Vaccines, and has a cross protection effect to viruses of the three pedigrees, so that the vaccine is a novel immune-enhanced O-type foot-and-mouth genetic engineering mixed epitope vaccine.

Description

technical field [0001] The invention belongs to the technical field of veterinary medicines, and relates to a foot-and-mouth disease genetic engineering mixed epitope vaccine and a preparation method thereof, an immune-enhancing O-type foot-and-mouth disease genetic engineering mixed epitope vaccine and a preparation method thereof. Background technique [0002] Foot-and-Mouth Disease (FMD) is a viral disease that seriously harms cloven-hoofed animals. It is listed as a severe infectious disease that must be notified by the World Organization for Animal Health (OIE), and it is listed as a first-class animal infectious disease in China. . The disease mainly infects cloven-hoofed animals such as pigs, cattle, and sheep, and has many transmission routes and fast transmission speeds. [0003] The causative agent of FMD is foot-and-mouth disease virus (FMDV), which belongs to the genus Aphthovirus of the family Picornaviridae. FMDV has seven serotypes, namely O, A, C, Asia 1, S...

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Problems solved by technology

At the same time, in the production process of high-efficiency vaccines, virus antigens must be concentrated, so the production and preparation costs of vaccines are relatively high

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