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A kind of foot-and-mouth disease genetic engineering mixed epitope vaccine and its preparation method

A technology of genetic engineering and epitope vaccine, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, and antibody medical ingredients, etc., and can solve the problem of high cost of vaccine production and preparation

Active Publication Date: 2015-07-29
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, in the production process of high-efficiency vaccines, virus antigens must be concentrated, so the production and preparation costs of vaccines are relatively high

Method used

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  • A kind of foot-and-mouth disease genetic engineering mixed epitope vaccine and its preparation method
  • A kind of foot-and-mouth disease genetic engineering mixed epitope vaccine and its preparation method
  • A kind of foot-and-mouth disease genetic engineering mixed epitope vaccine and its preparation method

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Embodiment Construction

[0017] The technical solution of the present invention will be described in detail below in conjunction with specific embodiments of the accompanying drawings.

[0018] Materials and Methods

[0019] Design and expression of B cell immunogen BI

[0020] According to the epidemiological information of O-type foot-and-mouth disease at home and abroad, the B cell immunogen gene BI was designed and synthesized. The neutralizing antigenic epitope sequence of O-type foot-and-mouth disease virus contained in it is shown in Table 1 below. The amino acid sequence in Table 1 is as follows Arrange and combine in sequence: PADRE-GG-(O / HN / CHA / 09)VP1 132-160-GG-VP1 193-211-GG-(O / Tibet / 99)VP1132-160-GG-(O / IRN / 2010) VP1 193-211-GG-VP1 132-160-GG-(O / Mya98) VP1132-160-GG-VP1 193-211-GG-Invasin-6×His, each epitope is linked by two glycines , and finally add a 6-histidine tag sequence, which is beneficial to the purification of the expressed protein. The designed sequence was submitted to Nan...

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Abstract

The invention discloses a foot-and-mouth disease genetic engineering mixed epitope vaccine and a preparation method thereof. The vaccine consists of the following four parts: a serial B cell epitope recombinant protein BI consisting of main neutralizing epitops of O-type foot-and-mouth disease viruses in Cathay, Transasia and Mya 98 pedigrees with a gene sequence of SEQ ID NO:1 and an amino acid sequence of SEQ ID NO:2, a T-cell epitope recombinant protein TI consisting of serial connection of universal T-cell epitope and a plurality of foot-and-mouth disease virus specific T-cell epitopes with a gene sequence of SEQ ID NO:3 and an amino acid sequence of SEQ ID NO:4, Toll-like receptor 3 agonist-polyinosinic acid-polycytidysic acid and / or Toll-like receptor7 / 8 agonist-R848 serving as immunopotentiator, and 201 oil adjuvant. When being used for immunizing a pig, the BI and TI mixed epitope vaccine prepared by utilizing the method can produce a protective immunization effect the same as or better than that of an inactivated influenza virus Vaccines, and has a cross protection effect to viruses of the three pedigrees, so that the vaccine is a novel immune-enhanced O-type foot-and-mouth genetic engineering mixed epitope vaccine.

Description

technical field [0001] The invention belongs to the technical field of veterinary medicines, and relates to a foot-and-mouth disease genetic engineering mixed epitope vaccine and a preparation method thereof, an immune-enhancing O-type foot-and-mouth disease genetic engineering mixed epitope vaccine and a preparation method thereof. Background technique [0002] Foot-and-Mouth Disease (FMD) is a viral disease that seriously harms cloven-hoofed animals. It is listed as a severe infectious disease that must be notified by the World Organization for Animal Health (OIE), and it is listed as a first-class animal infectious disease in China. . The disease mainly infects cloven-hoofed animals such as pigs, cattle, and sheep, and has many transmission routes and fast transmission speeds. [0003] The causative agent of FMD is foot-and-mouth disease virus (FMDV), which belongs to the genus Aphthovirus of the family Picornaviridae. FMDV has seven serotypes, namely O, A, C, Asia 1, S...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61K39/135A61P31/14
Inventor 卢曾军刘在新曹轶梅孙普李冬包慧芳李平花付元芳陈白兴文应理谢宝霞
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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