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A kind of compound vaccine adjuvant composition

A vaccine composition and the technology of the composition, which are applied in the directions of microorganisms, antiviral agents, biochemical equipment and methods, can solve the problems of poor cross-protection effect, rapid mutation of porcine reproductive and respiratory syndrome, etc., and achieve the promotion of cellular immunity. Effect

Active Publication Date: 2021-07-23
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Porcine reproductive and respiratory syndrome mutates rapidly, and the cross-protection effect between different strains of porcine reproductive and respiratory syndrome virus is poor, and multiple strains co-circulate

Method used

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  • A kind of compound vaccine adjuvant composition
  • A kind of compound vaccine adjuvant composition
  • A kind of compound vaccine adjuvant composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, oil-in-water emulsion composition

[0052] Sucrose ester L195 10g (Mitsubishi-Kagaku Foods Co., Tokyo, Japan) was mixed with Tween-80 10g, white oil for veterinary injection 40g, 190g 0.01w / v% thimerosal (Sigma) phosphate buffered saline (PBS-thimerosal; pH7.0) were mixed. The mixture is emulsified with three passes through a homogenizer at ambient temperature and at an internal pressure of at least 400 bar. Each emulsion was examined under a microscope. If there are more than 10 oil droplets with a diameter greater than 1 μm per 10 examined fields under a microscope at 1000X magnification, the emulsification process is repeated. The resulting emulsion was stored at 4°C until use.

Embodiment 2

[0053] Embodiment 2, PICKCa solution

[0054] Double-strand polymyocyte freeze-dried powder (purchased from Hangzhou Meiya Pharmaceutical Co., Ltd.) 1g, dissolved in 100mL sterile PBS, 10mg / mL, take 15mL and add kanamycin 2.25g, anhydrous calcium chloride 0.555g for use Dilute to 150mL in PBS, and prepare 1mg / mL PICKCa solution.

Embodiment 3

[0055] Embodiment 3, DDA solution

[0056] Dimethyldicoctadecylammonium bromide (DDA; Fluka Analytical) was dissolved in ethanol to make a 15 mg / ml stock solution, and the DDA stock solution was filtered through a 0.2 micron filter.

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Abstract

The present invention relates to a kind of composite adjuvant composition and the vaccine composition containing this adjuvant, composite adjuvant composition contains oil-in-water emulsion, polyinosinic acid-polycytidylic acid or derivatives thereof and DDA, can significantly improve Cellular immunity to antigens.

Description

technical field [0001] The invention relates to a vaccine adjuvant composition, in particular to a compound vaccine adjuvant composition for animals. Background technique [0002] "Adjuvant" generally refers to any substance that increases the humoral or cellular immune response to an antigen. An adjuvant is used for two purposes: it slows the release of antigen from the injection site, and it stimulates the immune system; it allows smaller doses of antigen to be used to stimulate a similar immune response, reducing the cost of manufacturing the vaccine. Thus, the potency of certain antigens can be significantly increased when the antigen is combined with an adjuvant. [0003] Porcine reproductive and respiratory syndrome is caused by porcine reproductive and respiratory syndrome virus (porcine reproductive and respiratory syndrome virus, PRRSV), which is characterized by fever, abortion in sows, increased mortality of piglets before and after weaning, respiratory disorders...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/12A61K39/245A61P31/14A61P31/22
CPCA61K39/12A61K39/39A61K2039/55511A61K2039/55561A61K2039/55566A61K2039/55594A61K2039/572C12N2710/16734C12N2770/10034
Inventor 张许科孙进忠田克恭刘永梅
Owner PU LIKE BIO ENG
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