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Compound vaccine adjuvant composition

A vaccine composition and composition technology, applied in the direction of microorganisms, antiviral agents, biochemical equipment and methods, etc., can solve the problems of poor cross-protection effect, rapid variation of porcine reproductive and respiratory syndrome, etc.

Active Publication Date: 2017-12-08
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Porcine reproductive and respiratory syndrome mutates rapidly, and the cross-protection effect between different strains of porcine reproductive and respiratory syndrome virus is poor, and multiple strains co-circulate

Method used

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  • Compound vaccine adjuvant composition
  • Compound vaccine adjuvant composition
  • Compound vaccine adjuvant composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, water-in-oil emulsion composition

[0052] Sucrose ester L195 10g (Mitsubishi-Kagaku Foods Co., Tokyo, Japan) was mixed with Tween-80 10g, white oil for veterinary injection 40g, 190g 0.01w / v% thimerosal (Sigma) phosphate buffered saline (PBS-thimerosal; pH7.0) were mixed. The mixture is emulsified with three passes through a homogenizer at ambient temperature and at an internal pressure of at least 400 bar. Each emulsion was examined under a microscope. If there are more than 10 oil droplets with a diameter greater than 1 μm per 10 examined fields under a microscope at 1000X magnification, the emulsification process is repeated. The resulting emulsion was stored at 4°C until use.

Embodiment 2

[0053] Embodiment 2, PICKCa solution

[0054] Double-strand polymyocyte freeze-dried powder (purchased from Hangzhou Meiya Pharmaceutical Co., Ltd.) 1g, dissolved in 100mL sterile PBS, 10mg / mL, take 15mL, add kanamycin 2.25g, anhydrous calcium chloride Dilute 0.555g to 150mL with PBS, and prepare 1mg / mL PICKCa solution.

Embodiment 3

[0055] Embodiment 3, DDA solution

[0056] Dimethyldicoctadecylammonium bromide (DDA; Fluka Analytical) was dissolved in ethanol to make a 15 mg / ml stock solution, and the DDA stock solution was filtered through a 0.2 micron filter.

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PUM

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Abstract

The invention relates to a compound adjuvant composition and a vaccine composition containing the adjuvant. The compound adjuvant composition consists of an oil-in-water emulsion, polyinosinic acid-polycytidysic acid or derivatives thereof and DDA. Cellular immunity of antigen can be obviously improved.

Description

technical field [0001] The invention relates to a vaccine adjuvant composition, in particular to a compound vaccine adjuvant composition for animals. Background technique [0002] "Adjuvant" generally refers to any substance that increases the humoral or cellular immune response to an antigen. An adjuvant is used for two purposes: it slows the release of antigen from the injection site, and it stimulates the immune system; it allows smaller doses of antigen to be used to stimulate a similar immune response, reducing the cost of manufacturing the vaccine. Thus, the potency of certain antigens can be significantly increased when the antigen is combined with an adjuvant. [0003] Porcine reproductive and respiratory syndrome is caused by porcine reproductive and respiratory syndrome virus (porcinereproductive and respiratory syndrome virus, PRRSV), which is characterized by fever, abortion in sows, increased mortality of piglets before and after weaning, respiratory disorders ...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/12A61K39/245A61P31/14A61P31/22
CPCA61K39/12A61K39/39A61K2039/55511A61K2039/55561A61K2039/55566A61K2039/55594A61K2039/572C12N2710/16734C12N2770/10034
Inventor 张许科孙进忠田克恭刘永梅
Owner PU LIKE BIO ENG
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