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Method for establishing acute viral hepatitis animal model

A viral hepatitis and animal model technology, applied in the fields of liver immunology and experimental zoology, can solve the problems of poor effect of acute liver injury and achieve good results

Inactive Publication Date: 2015-06-17
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the above disadvantages, provide a method for establishing an animal model of acute viral hepatitis, and solve the technical problem in the prior art that the effect of simulating acute liver injury caused by viral infection is not good

Method used

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  • Method for establishing acute viral hepatitis animal model
  • Method for establishing acute viral hepatitis animal model
  • Method for establishing acute viral hepatitis animal model

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Experimental program
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Embodiment 1

[0032] Embodiment 1 of the present invention provides a method for establishing an animal model of acute viral hepatitis, such as figure 1 shown, including the following steps:

[0033] Step S101: Preparation of different pure strains of mice:

[0034] From pure-line white mice (pure-line BALB / c mice), pure-line B6 mice (pure-line C57BL / 6J mice), pure-line severe combined immunodeficiency (SCID) mice, pure-line nude mice (pure-line C57BL / 6J mice) At least one group of mice was selected from nude mice with BALB / c background) or pure line differentiation antigen 1d (CD) deficient mice (mice with pure line BALB / c background), wherein the age of mice was 6-8 weeks Zhou, the animal model established by mice of this age has high symptom stability, and it is relatively easy to simulate human diseases. The following experiments all use female mice. The mice are kept in a pathogen-free animal room, and the feed and water are sterilized. Prevent viral infection.

[0035] Step S102: D-g...

Embodiment 2

[0047] Embodiment 2 of the present invention provides a method for establishing an animal model of acute viral hepatitis, comprising the following steps:

[0048] Step S201: Preparation of different pure strains of mice:

[0049] Pure-line white mice (pure-line BALB / c mice) were selected, aged 6-8 weeks, female. The mice were kept in a pathogen-free animal room, and the feed and water were sterilized to prevent virus infection.

[0050] Step S202: D-galactosamine, polyinosinic acid: preparation of polycytidylic acid:

[0051] Prepare D-galactosamine solution with sterile phosphate buffer solution (PBS solution), prepare polyinosinic acid:polycytidylic acid solution with sterile phosphate buffer solution, wherein, the final concentration of D-galactosamine solution is respectively 20 μg / μl, polyinosinic acid: polycytidylic acid solution final concentration is 10ng / μl, D-galactosamine and polyinosinic acid: polycytidylic acid are all purchased from Sigama Company in the United ...

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Abstract

The present invention relates to the technical field of liver immunology and experimental zoology, particularly to a method for establishing an acute viral hepatitis animal model, wherein D-galactosamine and polyinosinic acid:polycytidylic acid are injected in a peritoneal injection or intravenous injection manner, the D-galactosamine and the polyinosinic acid:polycytidylic acid produce the synergy effect, the ALT activity change is detected, and the acute viral hepatitis animal model is established. With the method of the present invention, the hepatitis symptoms are rapidly induced, and the viral infection simulation effect is good.

Description

【Technical field】 [0001] The invention relates to the technical fields of liver immunology and experimental zoology, in particular to a method for establishing an animal model of acute viral hepatitis. 【Background technique】 [0002] Hepatitis is a disease that seriously endangers people's life and health and has a high mortality rate. The etiology mainly includes the destruction of liver parenchymal cells caused by viral infection, excessive alcohol intake and ingestion of chemical poisons. Its pathogenic mechanism is complex. Due to the lack of human liver samples and the limitations of in vitro research, it is of great practical significance to establish experimental liver injury animal models to simulate human liver diseases, study the pathogenesis of hepatitis, and screen drugs for the treatment of hepatitis. [0003] Among them, liver immune injury has attracted much attention, especially the pathological injury involving liver lymphocytes has become a crucial factor i...

Claims

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Application Information

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IPC IPC(8): A61K31/787A61K31/7008
Inventor 万晓春田永帅陈凤莲
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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