72 results about "Phenome" patented technology

The present invention relates to compounds for treatment that protects the endothelium, prevents pathologic thrombus formation in the microcirculation and preserves platelet number and function and thus may be related to treatment or prevention of ischemic events in patients with cardiovascular disease. The present invention is particularly useful for patients having or being at increased risk of development of an ischemic event such as an acute myocardial infarction and / or no-reflow phenomena and / or ischemia-reperfusion injury by administration of agent(s) modulating and / or preserving endothelial integrity. The compounds may be administered in combination with standard treatment of acute cardiovascular ischemic events such as Platelet inhibitors such as aspirin (ASA), Thienopyridins, GPIIb / IIIa inhibitors), Parenteral anticoagulants such as unfractioned heparin (UFH), bivalirudin, enoxaparin, and fondaparinux, Verapamil, Adenosine, Sodium nitroprusside, Nitroglycerin, Epinephrine, Beta-blockers and surgical methods such as percutaneous coronary intervention (PCI), PCI with thrombus aspiration, PCI with stents.

The invention discloses a computer aided model construction method based on deep learning gastric cancer pathological sections, and belongs to the technical field of artificial intelligence. The method uses a 121-layer dense-connected convolutional neural network to perform image recognition. A dense block structure in a DenseNet allows the high-level part of the network to acquire shallow features, which greatly reduces the over-fitting phenomenon. At the same time, the model has a large number of layers, which can fit more complex and smoother decision functions. Although the number of layers is large, the number of parameters of the model is not large, which saves resource consumption. In order to further avoid over-fitting, a training mechanism for migration learning is adopted. The model will be pre-trained on an ImageNet dataset to give the model a strong image feature extraction capability. The main optimization of the model during formal training can be better focused on how toextract the features of the diseased area, and the utilization rate of the data is greatly improved.

The object of this invention is to elucidate the factors involved in the regulation of AGE (advanced glycation endproducts, which are produced in a living body accompanied with diabetes and aging) and RAGE (the receptor for AGE), in order to facilitate investigation on the biological activities, physiological phenomenon and diseases related to AGE and RAGE. Plural molecular species are known to exist for RAGE, and among such molecular species, soluble RAGE exhibits the activity to modulate interaction between AGE and transmembrane-type RAGE. Using this soluble RAGE or a nucleic acid encoding it, the soluble RAGE can be measured, in addition, investigation on various physiological phenomenon, biological activities, and diseases related to interaction between AGE and RAGE can be performed to facilitate development a medicine having further efficacy.

The present invention discloses an improved deep Boltzmann machine-based pulmonary nodule feature extraction and benign and malignant classification method. The method includes the following steps that: step A, pulmonary nodules are segmented from CT images through using a threshold probability image graph method, so that regions of interest (ROI) are obtained, and the regions of interest are cut into nodule images of the same size; and step B, a supervised deep learning algorithm Pnd-EBM is designed to realize the diagnosis of a pulmonary nodule, wherein the diagnosis of the pulmonary nodule further includes three major steps: B1, a deep Boltzmann machine (DBM) is adopted to extract the features of the ROI of the pulmonary nodule which have deep expression abilities; B2, a sparse cross-entropy penalty factor is adopted to improve a cost function, so that the phenomenon of feature homogenization in a training process can be avoided; and B3, an extreme learning machine (ELM) is adopted to perform benign and malignant classification on the extracted features of the pulmonary nodule. The improved deep Boltzmann machine-based pulmonary nodule feature extraction method is superior to a traditional feature extraction method. With the method adopted, the complexity of manual extraction and the difference of feature selection can be avoided, and references can be provided for clinical diagnosis.

The invention relates to a DCNN (Deep Convolutional Neural Network)-DNN (Deep Neural Network) and PV-SVM (Paragraph Vector-Support Vector Machine)-based multi-modal depressive disorder estimation and classification method. The method comprises the following steps: preprocessing audio and video features through a displacement range histogram and an Opensmile tool, extracting hidden layer abstract features of audio and video statistical features through a DCNN, performing depressive disorder estimation through a DNN, performing high-dimensional feature mapping on textile information through a PV method, inputting an obtained high-dimensional feature expression into an SVM for binary classification, connecting a depressive disorder estimation result with a binary classification result in series, inputting the whole into a random forests model for training, and performing a final depressive disorder classification task through the trained random forests model, namely judging a depressive disorder or a non depressive disorder. By the adoption of a DCNN model for extraction of the hidden layer abstract features from a primary audio / video, an original high-dimensional feature is more compact, and included information is richer; therefore, the model is more effective, and the phenomenon of overfitting caused by extremely high dimension of the feature is avoided.

The object of this invention is to elucidate the factors involved in the regulation of AGE (advanced glycation endproducts, which are produced in a living body accompanied with diabetes and aging) and RAGE (the receptor for AGE), in order to facilitate investigation on the biological activities, physiological phenomenon and diseases related to AGE and RAGE. Plural molecular species are known to exist for RAGE, and among such molecular species, soluble RAGE exhibits the activity to modulate interaction between AGE and transmembrane-type RAGE. Using this soluble RAGE or a nucleic acid encoding it, the soluble RAGE can be measured, in addition, investigation on various physiological phenomenon, biological activities, and diseases related to interaction between AGE and RAGE can be performed to facilitate development a medicine having further efficacy.

The invention discloses application of demethylation modification of targeted mRNA by using PspCas13b in combination with m6A demethylase Alkbh5. A study shows that a PspCas13b-Alkbh5 fusion protein can perform demethylation modification by targeting target gene (such as CYB5A and CTNNB1) mRNA through the specific gRNA to further regulate the target gene expression. m6A RNA immunoprecipitation assays and m6A site quantitative PCR results show that the PspCas13b-Alkbh5 fusion protein can effectively reduce the m6A methylation level of the target gene mRNA in the cell, and no off-target phenomenon is found. The PspCas13b is used as a means and combined with the m6A demethylase Alkbh5 to achieve the demethylation modification of the targeted mRNA for the first time and regulate the expressionof the specific purpose and application accordingly. The method breaks through the previous specific protein expression regulation based on previous change of DNA genetic information, provides a newpotential treatment mode and strategy for treatment of various diseases in the future, and has a good application prospect.

The invention discloses a molecular marker closely linked with an oil content character of the rapes and an application of the molecular marker. The molecular marker BrSF34-123 closely linked with the oil content character of the rapes comprises the following screening steps: 1) hybridizing a cabbage type rape variety zy036 and 51070 so as to obtain a DH generation segregation population; 2) designing a primer to polymorphically screen a parent, and establishing a genetic linkage map; 3) carrying out an field experiment and harvesting for the DH generation segregation population to obtain a phenome database of the oil content; and 4) carrying out QTL (quantitative trait locus) detection by combining the genetic linkage map of the developed high-density molecular marker with a genotype of the segregation population and the phenome database of the oil content to obtain the molecular marker closely linked with the oil content character of rapes. Therefore, the marker is utilized to be conducted for marker-assisted selection, thereby being capable of quickly screening the strains with high oil content for breeding the oil content of the rapes, being definite in breeding selection target and saving cost.

The invention discloses a subject knowledge expression and description method. Starting from fusion of multiple subjects, expressions and descriptions of knowledge from complicated relationship to simplified relationship are searched with the basic theory of psychological cognition acting as guidance, and the phenomenon of influence of complicated relationship on cognition under mind mapping and other modes, especially cognition of subject knowledge of primary and secondary schools, can be solved. Factors of hierarchical cognition, association relation cognition, type cognition and knowledge information content are comprehensively considered, the expressions of the four factors are combined and the complete expressions of the knowledge can be obtained from different aspects. The relationship of knowledge cognition and geographical cognition is put forward firstly, and an automatic conversion and mapping method from a knowledge space to a map space is considered so that the application blank is filled.

The invention provides a method for performing in-vitro accurate test on KRAS gene mutation, and the method is used for overcoming the defects that the conventional allele-specific polymerase chain reaction (PCR) method has insufficient specificity and false positive phenomenon. The method for performing the in-vitro accurate test on the KRAS gene mutation comprises the following steps of: (1) designing and synthetizing allele specific primers which comprise the allele specific primers espectively designed for 7 types of mutation of a typical KRAS gene exon 2 and are utilized as forward primers, wherein extension retardant primers are utilized as common downstream primers; (2) extracting the KRAS genomic deoxyribose nucleic acid (DNA) of a test sample, and additionally preparing the wild type genomic DNA (as wild type template); and (3) carrying out the real-time fluorescent PCR reaction. The test method has the advantages of strong specificity, high sensitivity, material conservation and time-saving property.

A mass notification terminal may have a data parser and decoder connected to a communications terminal. Announcements may be transmitted to the communications terminal in the form of linguistic symbols and commands using low bandwidth and low power protocol transmissions. Push transmissions conserve bandwidth. An abstraction of an audio announcement may be transmitted for use with a speech synthesizer. The abstraction may be linguistic symbols such as phenomes, text, or may identify pre-stored clips. The system may provide announcement confirmation. The system may take advantage of communication protocols that have message size limitations. The announcements may be sent in one or more message transmissions. When an announcement is composed of multiple messages, using message sequence numbers and announcement identifications may facilitate grouping and arranging of the messages that make up the announcement.

The invention discloses a blended yarn spinning method. Blended yarn is composed of cashmere, synthetic fiber and viscose acetal fiber, and spinning is finished through manual blending, blowing, cotton carding, drawing and roving. The method has the advantages that manual blending is adopted for mixing of fiber, and the nonuniformity of cashmere in finished yarn is solved; the process that carding is conducted more times than striking in the blowing and cotton carding processes, anti-static coating powder is added, damage to cashmere fiber and fleece falling are reduced, and the phenomenon that cashmere winds the cylinder and doffer easily is solved; the twist of roving is increased, and the defects that adhering, breakage and loosening, caused by the features that the crispation number of cashmere is reduced, and the fiber is smooth, in the processing process are overcome.

The invention discloses applications of a CvBV26-4 gene in the reduction of the immunity of fruit flies and the preparation of an immunocompromised fly model. The CvBV26-4 gene is a fourth gene on the 26th loop in 35 genome fragments of Cotesia plutellae polydanvirus DNA, and the base sequence of the CvBV26-4 gene is represented by SEQ ID NO. 1. The Cotesia plutellae polydanvirus CvBV26-4 gene is transferred to a fruit fly genome by using a transgenic technology to obtain a stable-inheritance homozygotic transgenic fruit fly strain; and the transgenic strain has a blood corpuscle apoptosis phenomenon after expressing the polydanvirus DNA gene with CvBV26-4, has obvious low immunity after being invaded by pathogens, and is of great significance to research the insect immunization correlated mechanism and enhance and screen human immunity enhancing medicines.

The invention discloses a construction method of Slco1b2 gene knockout rat and an application thereof. According to the invention, construction of Slco1b2 gene knockout rat is carried out by using a CRISPR / Cas9 system, which includes selection of Slco1b2 knockout targets, in-vitro synthesis and transcription of sgRNA and Cas9 mRNA, preparation of pseudopregnant mother rats, in-vitro microinjectionand transplantation of single cell embryos, rat breeding and screening, and obtaining of homozygote Slco1b2 gene knockout rat. Through verification by T7EI endonuclease, the gene knockout rat prepared by the method of the invention does not generate off-target phenomenon, and in the F2-generation knockout rat of about 4 weeks, total bilirubin, direct bilirubin and indirect bilirubin content are significantly higher than that of wild type rats. The invention also proposes an application of the construction method for hyperbilirubinemia research and drug development. The method provides an effective method for researching diseases such as increased bilirubin, and provides an effective experimental tool for drug research and development, and has broad application prospects.

The invention discloses a major group recommendation and college entrance examination admission simulation filling method for candidates in Shanghai. The method mainly includes inputting the college entrance examination scores and the selected courses of candidates, and matching appropriate major groups in a college major group pool according to the two items of information, and analyzing the historical college student enrollment score data to assess the risk of the candidates enrolling in the major group; according to the risk of the enrolled major group, further dividing the appropriate major groups into three categories: a struggle group, a stable group, and an insurance group; enabling each candidate to select out 24 major groups (96 majors) based on the three types of college major groups produced by the system, after the candidates selects out the college major groups, re-adjusting the order of the major groups according to pre-customized preference models or expert guidance. Theinvention firstly provides and implements a method for the candidates in Shanghai to fill in college admission sheets so as to reduce the phenomena that the candidates have no admission to colleges.

The invention discloses application of CvBv5-3 gene to reduction of immunity of fruit fly and preparation of fruit fly model with low immunity. The CvBv5-3 gene is the third gene of the fifth ring of the 35th genome segment in polydnavirus of a cotesia vestalis, and a base sequence of the CvBv5-3 gene is shown as SEQ ID NO. 1. According to the application, the CvBv5-3 gene in polydnavirus of the cotesia vestalis is transferred into fruit fly strains, and transgenic fruit fly strains with stable inheritance and homozygote are obtained. After the transgenic fruit fly strains express the polydnavirus gene CvBv5-3, the phenomenon of blood cell apoptosis exists. In addition, when being infected by pathogen, the transgenic fruit fly strains has an obvious character of low immunity, and has important meaning in the aspects of research of relevant mechanism of insect immunity and enhancement of human immunological medicaments screening and the like.

The embodiment of the invention relates to the technical field of composite plates and discloses a method for machining a composite plate and a cover plate. The method for machining the composite plate comprises the following steps that a staining liquid is prepared according to a target color to be machined; after ultraviolet (UV) transferring is conducted on the composite plate, the composite plate subjected to UV transferring is put into the staining liquid prepared so that dip dyeing coloring can be conducted; and the composite plate colored is acquired. By means of the method for machining the composite plate, when the composite plate is colored, the phenomena of cracking, membrane explosion and cracks can be prevented from occurring.

The invention discloses new application of P53 gene mutation and telomere dysfunction, namely, application of a reagent for detecting cancer suppressor gene P53 gene mutation and telomere dysfunction in preparing a reagent for clinical diagnosis of polycystic kidney disease, or the application of P53 gene mutation and telomere dysfunction in screening the therapeutics for the polycystic kidney disease. An experimental result shows that the hybrid descendant of the mice with p53N236S gene mutation and the mice suffering from Werner syndrome (WS) suffers from the polycystic kidney disease so that a novel PKD disease model is acquired. According to the application, the condition that the happening of the polycystic kidney disease possibly is a new phenomenon of abnormal proliferation of kidney cells caused by the combined action of mutation p53 and aging signal is discovered for the first time. Through the discovery, the pathogenesis of the polycystic kidney disease is enriched, and a new biomarker for the clinical diagnosis of the polycystic kidney disease is provided.

The purpose of the present invention is to develop a cucumber fruit mottle mosaic virus vector so as to enable a study of gene function through gene silencing phenomenon in Cucurbitaceae plants, which has been difficult to study in the past, and to enable the study in fruits as well through stable expression. Furthermore, the present invention establishes a system that enables expression of a heterologous protein in plants, thereby, for the first time, providing a vector applicable to both the gene silencing phenomenon and protein expression in Cucurbtaceae plants.

The invention discloses a DNA marker plasmid and preparation and application thereof. The DNA marker plasmid is a double-chain closed circular DNA molecule, and the nucleotide sequence of the DNA marker plasmid is SEQ ID No:3. Because the high-copy replicon pUC ori is selected, the plasmid extracting rate is improved, and because of high changeability of complete sequence by artificially synthesized, the recombinant DNA marker plasmid does not contain repeat sequences, thereby the plasmid stability is improved and the recombinant DNA marker plasmid can cover the most digestion phenomenon possibilities by the minimum length.

Disclosed is a null mutant (or knockout) rodent comprising in its germ cells an artificially induced PTTG null mutation. In some embodiments, the null mutant rodent can be generated by way of homologous recombination in an embryonic stem cell or germ cell. The inventive null mutant rodent can be used to study mammalian physiology at the cellular, tissue, and / or organismal level with respect to various phenotypes, including hyperglycemia, hypoinsulinaemia, hypoleptinemia, diabetes, chromosomal aneuploidy, premature centromere division, chromosomal damage, aberrant mitotic cellular division, thrombocytopenia, thymic hyperplasia, splenic hypoplasia, testicular hypoplasia, and female subfertility. Also disclosed is an animal model for diabetes. Also disclosed is a somatic or germ cell obtained from the null mutant rodent. Also disclosed is a cell line derived from a cell obtained from the null mutant rodent.

The invention belongs to the field of gene engineering and relates to a pre-T vector and a T vector composed of the pre-T vector and application thereof. The improved promoter is a recognition site for mutation from a nucleotide sequence from -35 region to -10 region in a promoter region into endonuclease. By the T vector, the problem that the product of transcription or translation of exogenous genes by a strong promoter during blue-white selection of a vector might be toxic to host and cloning will fail can be overcome, blue-white selection of a vector might be toxic to host as a strong promoter starts transcription or translation; the defect that delection of 1-2 bp at enzyme digestion site leads to lacZalpha gene frameshift mutation and false positive clones are generated can be avoided; and the false negative phenomenon that a plate is full of blue stains because exogenous DNA fragment is small and insertion of exogenous DNA does not change a reading frame of lacZalpha gene can beeliminated.

The invention discloses a conjugate, which includes a mutant syphilis detection antigen and a marker. Through gene mutation of the syphilis detection antigen, one or more lysine in the middle of the syphilis detection antigen can be mutated. The lysine easy to combine with the marker in the middle of the syphilis detection antigen turns into other amino acids, so that the marker is marked at a lysine site at the N terminal or C terminal of the mutant syphilis detection antigen and does not combine with the middle part amino acid, thus avoiding wrapping of the antigen active site by the marker. Compared with traditional reagents, the antigen active site of the conjugate is not wrapped by the marker, the antigenic activity decrease caused by combination of antigen determinants and the marker can be avoided, thereby not affecting the antigen activity. The conjugate can improve the detection sensitivity and specificity when used for syphilis detection, and can avoid the prozone phenomenon. The invention also discloses the preparation method and application of the conjugate.

The invention relates to the field of microorganisms, and concretely provides a duck-derived coronavirus attenuated strain IBV DCV35 (with the preservation number being CGMCC NO.13852) and an application thereof. The duck-derived coronavirus attenuated strain IBV DCV35 is obtained through passage attenuation screening of a duck-derived coronavirus virulent strain IBV ZV2004 strain (with the preservation number being CGMCC NO.3842), the S1 gene sequence of the attenuated strain IBV DCV35 is significantly mutated, and the mutated sequence can be stably inherited in the 22-35th generations. SPF chickens are infected with the screened attenuated strain IBV DCV35 by artificial infection test and horizontal transmission test, and no virulence return phenomenon is observed. Experiments show thatthe attenuated strain can effectively activate the immune system in chickens and well prevent avian infectious bronchitis after being inoculated into the chickens, and can be used as a vaccine for preventing the avian infectious bronchitis.

The invention discloses an SNP mark for evaluating a genetic background infiltration phenomenon of rhesus monkeys in cynomolgus monkeys and application of the SNP mark. The SNP mark comprises 48 SNP loci, namely SNP1-SNP48 which are respectively located at loci 151 in nucleotide sequences represented by SEQ ID NO:1-SEQ ID:NO48. By utilizing advantages of biological information techniques, the SNP mark for evaluating the genetic background infiltration phenomenon of the rhesus monkeys in the cynomolgus monkeys is developed and is applied to the evaluation of a genetic background infiltration level of the rhesus monkeys in the cynomolgus monkeys, so that the cost is greatly lowered, and the operation is simplified. By applying the SNP mark, rapid preliminary screening can be realized, the detection cost is lowered, and therefore, the standardized process of the evaluation of the genetic background infiltration phenomenon of the rhesus monkeys in the cynomolgus monkeys is promoted.