66 results about "Near infrared excitation" patented technology

The invention belongs to the technical field of nano biological materials, and particularly relates to an 800nm-near-infrared-excited 1525nm-shortwave-infrared-emission fluorescence nano material and a synthesis method thereof. The fluorescence nano material is a one-core / three-shell core-shell structure nanocrystal material composed of a nucleating center, a shortwave infrared light-emitting layer, an energy transmission layer and an energy absorption layer, wherein the nucleating center provides a crystal core for growth of the other three layers and limits the particle size of the nanocrystal; the shortwave infrared light-emitting layer is used for absorbing exciting light with specific wavelength and emitting shortwave infrared light; the energy transmission layer is used for transmitting energy between the energy absorption layer and shortwave infrared light-emitting layer; and the energy absorption layer is used for absorbing energy and transferring the energy to the energy transmission layer. The three-layer structure design widens the exciting light from 980nm to 800nm or so, and implements the Nd<3+>->Yb<3+>->Er<3+> energy transfer process. Due to the ideal excitation and emission wavelengths, the material can be used for deep tissue in-vivo imaging.

The invention discloses a preparation method of a gold-silver composite nanoring. The preparation method comprises the following steps: placing a silver target material in water while stirring, and irradiating the silver target material for 10-30 minutes by using laser with the wavelength of 1064nm, the power of 30-50mJ / pulse, the frequency of 5-15Hz and the pulse width of 5-15ns to obtain a silver nanoparticle colloid solution; then, irradiating the silver nanoparticle colloid solution for 2-4 minutes while stirring by using laser with the wavelength of 532nm, the power of 34-38mJ / pulse, the power of 5-15Hz and the pulse width of 5-15ns to obtain a monodisperse silver nanoparticle colloid solution; then, adding the monodisperse silver nanoparticle colloid solution to a 0.13-0.17mmol / L chloroauric acid according to a volume rate being (0.8-1.2): (0.8-1.2), and stirring for at least 30 minutes to prepare the gold-silver composite nanoring in which a silver nanoring is modified with gold nanoparticles, wherein the ring diameter of the silver nanoring is 10-25nm, the thickness of the silver nanoring is 3-6nm and the particle diameter of the gold nanoparticles is 3-5nm. The gold-silver composite nanoring prepared by the preparation method disclosed by the invention is expected to be applied to a Raman detection technology based on a near-infrared excitation SERS (Surface Enhanced Raman Scattering) effect.

A silicon dioxide coated conversion fluorescent nanometer crystal on rare-earth core shell by direct in-situ synthesizing and its production of core-shell nanometer crystal are disclosed. The general formula is AxBy / SiO2. It adopts reversed-phase aggregation micro-emulsion method combined with hydrothermal method. Nanometer crystal of ytterbium and thulium co-doped is used to transmit blue light, co-doped ytterbium and erbium is used to transmit green light and red light under near-infrared excitation. It's convenient and simplified; it has uniform size distribution, dispersion and repeating ability. It can be used as biological-inspecting and analyzing fluorescent marks.

The invention discloses a preparation method of carbon nanoparticles with controllable photoluminescence. In the method, the hydrothermal process is adopted; and the method comprises the following steps: preparing a solution composed of a carbohydrate which is used as a carbon source and an oxidation additive, feeding the solution to a hydrothermal reactor and heating to allow reactions; carrying out centrifugal separation on the reaction product, washing with water for three times and washing with ethanol for three times; removing salts; and drying to obtain carbon nanoparticles. The method provided by the invention is simple and low in cost. The prepared carbon nanoparticles have good dispersibility, contain abundant surface oxygen-containing groups (such as hydroxyl groups and carboxyl groups) which make surface functionalization or modification of carbon nanoparticles easier, and are excellent in fluorescence properties of ultraviolet-visible excited visible emission fluorescence properties, up-conversion fluorescence properties and near-infrared excitated near-infrared emission properties. The emission spectrum of carbon nanoparticles varies with the change in excitation wavelength.

This invention provides a novel bioimaging technique that can achieve a deep observation depth and a novel method for marking a lesion that allows clear recognition of the lesion from outside a living body. This invention also provides a bioimaging marker comprising a fluorescent material obtained by doping a ceramic with rare earths and the like and a bioimaging technique comprising detecting near-infrared fluorescence that can sufficiently penetrate a living body generated upon excitation of the marker with near-infrared excitation light.

The invention provides a side flow test paper strip for serum marker luminescence detection based on near infrared excitation and emission and preparation and use methods thereof, and relates to a side flow test paper strip and preparation and use methods thereof. The technical problems of heat damage of the existing detection material on biomolecules and high background signals can be solved. Theside flow test paper trip consists of a polyvinyl chloride support back plate, a detection pad, a combination pad, a sample pad and an absorption pad, wherein the combination pad is loaded with an upper conversion beta-NaYF4:Yb,Tm@NaXF4 nanometer crystal probe combined with antibodies and an upper conversion beta-NaYF4:Yb,Tm@NaXF4 nanometer crystal probe combined with mouse IgG. The preparation method comprises the following steps of 1, preparing a core; 2, coating a shell; 3, assembling a luminous probe; 4, assembling the side flow test paper strip. In the use process, the laser is used forirradiating the detection line to detect the emission spectrum; the detection range is 0.05 to 50ng / mL; the detection limit is 0.02ng / mL; the side flow test paper strip can be used in medical care detection.

The invention discloses a near infrared up-conversion long afterglow luminescent material and a preparation method thereof. The preparation method comprises the following steps: (1) weighing raw materials, and evenly mixing raw material powder, wherein the raw materials comprises a raw material A, a raw material B, a raw material C, and a raw material D, the raw material A is chromium oxides or corresponding salts, the raw material B is oxides of erbium or thulium or corresponding salts, the raw material C is ytterbium oxides or corresponding salts, and the raw material D is oxides of zinc, gallium / aluminum, or germanium / tin or corresponding salts; and (2) pressing and moulding mixed powder obtained in the step (1) to obtain a blank sample; (3) solid-phase sintering the blank sample obtained in the step (2) at a high temperature; and (4) cooling the sintering product to obtain the target material. A high temperature solid phase method is adopted, the prepared material, which is doped by Cr<3+> and Er<3+> / Tm<3+> and takes Yb<3+> as the sensitizing agent, has a near infrared excited up-conversion luminescence function and a super long time afterglow performance and can be used as a high performance functional material applied to related fields.

The invention relates to a preparation method and application of a rare earth up-conversion light-operated drug-delivery nano-carrier. The preparation method comprises the following steps: with a variety of rare earth salts as raw materials, preparing a rare earth up-conversion nanoparticle through a solvothermal process; synthesizing meso-porous silicon via a template process and carrying out coating; connecting a silane reagent to the surface of a meso-porous silicon shell layer; and finally, embedding a drug into meso pores. The nano-carrier prepared in the invention has a targeted aggregation effect and can realize precise positioning at a tumor site. The nano-carrier has a light-operated release effect; the drug is stored in the nano-carrier under the condition of no external near infrared laser (with wavelength of 980 nm) irradiation; the chemotherapy drug can only be released when a near infrared excitation source irradiates tumor tissue; thus, precise, timed and quantitative release of the nano-carrier at a tumor site can be realized. The nano-carrier has a fluorescence imaging function, can realize visualization of chemotherapy process and has the advantages of simple operation, good applicability, low cost and good dispersibility in water.

The invention relates to an 808 nanometer near-infrared excited composite antibacterial coating and a preparation method thereof. The invention utilizes a hydrothermal method to synthesize uniform size polyethylene glycol modified molybdenum sulfide nano-flowers in one pot. Furthermore, chitosan encapsulated in gentamicin loaded polyethylene glycol modified molybdenum sulfide nanoparticles slowedthe drug release rate. The advantages of the invention are: the petal-like molybdenum disulfide grown on titanium surface has a larger specific surface area which can increase the loading of gentamicin, Molybdenum disulfide itself has a good photothermal effect under near-infrared irradiation. The drug is released quickly under the photothermal effect. The synergistic effect of photothermal and gentamicin can achieve higher antibacterial efficiency in a short period of time.

The invention discloses a sheet-like magnetic infrared pigment. The pigment has a sheet-like sandwich structure formed by at least three layers A, B and C which are sequentially arranged from inside to outside. The layers A, B and C are a sheet-like magnetic pigment layer A, a connecting material layer B coated outside the sheet-like magnetic color layer, and a near-infrared absorption pigment or a near-infrared excitation pigment layer C coated outside the connecting material layer. The sheet-like magnetic infrared pigment disclosed by the invention combines magnetic and infrared anti-counterfeit signals, and can achieve the effect of oriented arrangement. Ink made of the pigment after being printed can achieve the 3D effect under the fixed magnetic action, for example, scroll bars, three-dimensional balls, plane slightly-embossed carving and the like. The sheet-like magnetic infrared pigment has a broad application value in the fields of anti-counterfeit technology and military affairs. Besides, the invention also discloses a method for preparing the sheet-like magnetic infrared pigment. The method is easy to operate, and the prepared sheet-like magnetic infrared pigment is stable.

The invention relates to the technical field of composites, in particular to an upconverting nanoparticle and graphene quantum dot composite capable of realizing near-infrared photodynamic therapy andfluorescence imaging simultaneously and a preparation method. According to the prepared composite, graphene quantum dots can be bonded together with upconverting nanoparticles, the composite is homogeneously spherical, and particle size is about 35 nm. Absorption spectra of the graphene quantum dots in the composite can overlap well with emission spectra of the upconverting nanoparticles, so thatenergy transfer is realized between the two materials. When the two materials make contact, the upconverting nanoparticles can absorb near-infrared light and emit ultraviolet light and visible lightunder irradiation of 980nm laser, the emitted ultraviolet light and visible light can be absorbed by the graphene quantum dots and subjected to a reaction with ambient oxygen, singlet oxygen with cytotoxicity is generated, and red light is emitted, so that photodynamic therapy and fluorescence imaging under near-infrared excitation are realized.

The invention discloses a spin crossover-upconversion nano composite material as well as a preparation method and application thereof, and belongs to the field of spin crossover complex materials. Thespin crossover-upconversion nano composite material is formed by combining a spin crossover complex and an upconversion nano luminous material through a coordination bond, and a structural formula isUCNPs@SCO, wherein SCO is the spin crossover complex; the spin crossover complex is an active functional group-containing single-core ferrous compound; UCNPs is the upconversion nano luminous material for exploring regulation and control of a spin crossover behavior by near infrared excitation. The method is easy to operate and mild in condition and can realize low-energy photoinduced spin crossover behavior at a solid-state unimolecule level; the material is applied to molecular electronic appliances related to information storage, molecular switching and molecular displaying.

The invention discloses a fluorescence depletion method and a microscopic imaging method and device. The fluorescence depletion method is characterized in that near-infrared depletion light is used to cause stimulated absorption in the sensitizing ions of rare earth doped upconversion nano materials, the energy of near-infrared excitation light is transferred to energy transfer ions, and depletion of the multiphoton fluorescence of the upconversion nano materials is achieved. The microscopic imaging method is provided on the basis of the fluorescence depletion method, and the microscopic imaging method is characterized in that the near-infrared depletion light is modulated into a hollow light beam by using a space phase modulation plate, and the hollow light beam and an excitation light beam are subjected to collimation conjugation focusing to achieve microscopic imaging of the rare earth doped upconversion nano materials and the marked samples of the rare earth doped upconversion nano materials. A super-resolution optical microscopic imaging system formed by an excitation light generating module, a depletion light generating module, a dichroscope, a multiphoton microscopic scanning module and a photoelectric detection module is built on the basis of the microscopic imaging method. By the fluorescence depletion method and the microscopic imaging method and system, low-cost, low-complexity, high-resolution, simple and effective real-time dynamic three-dimensional images can be obtained.

The invention discloses a luminous color variable upconversion nanometer luminescent material, which has a chemical expression of NaR0.8-x-yF4: Yb0.2, Hox, Tmy; in the chemical expression, R is at least one of Y, Gd and Ce, x is more than or equal to 0.0001 and less than or equal to 0.1, and y is more than or equal to 0.0005 and less than or equal to 0.1; the diameters of upconversion nanoparticles are 5-25nm. The material can be divided into two types according to the distribution mode of an activating agent: a single-core structure and a core-shell structure, wherein in the single-core structure, Tm and Ho are evenly distributed in nanoparticles, and in the core-shell structure, Tm and Ho are respectively distributed in a core and a shell. The invention also discloses a preparation method of the upconversion nanometer luminescent material. By using the surface effect of the upconversion nanometer luminescent material, the upconversion luminescence color of the material can change obviously along with the change of laser power density, temperature and radiation time under the condition of 980nm near infrared excitation, so that the material can be applied to the anti-counterfeit field.

The invention belongs to the field of small organic molecule fluorescent probes, and particularly relates to synthesis and biological application of a near-infrared excited and emitted mitochondria-targeted viscosity-sensitive fluorescent probe. The invention provides the mitochondria-targeted near-infrared excited and emitted viscosity fluorescent probe as shown in the following formula (1), and application of the fluorescent probe to detection of viscosity in a solution and detection of viscosity change of mitochondria in cells. The probe has near-infrared excitation and emission properties, can realize detection of viscosity in a solution and intracellular imaging, and has potential application value in the field of fluorescent biomarkers.

An improved system and method for reading an upconversion response from nanoparticle inks is provided. A is adapted to direct a near-infrared excitation wavelength at a readable indicia, resulting in a near-infrared emission wavelength created by the upconverting nanoparticle inks. A short pass filter may filter the near-infrared excitation wavelength. A camera is in operable communication with the short pass filter and receives the near-infrared emission wavelength of the readable indicia. The system may further include an integrated circuit adapted to receive the near-infrared emission wavelength from the camera and generate a corresponding signal. A readable application may be in operable communication with the integrated circuit. The readable application receives the corresponding signal, manipulates the signal, decodes the signal into an output, and displays and / or stores the output.

The invention discloses preparation of a near-infrared fluorescent probe with a PTT effect and an aggregation-induced emission enhancement effect, wherein the fluorescent probe is formed by linking atraditional fluorescent nuclear IR-780 iodide with near-infrared excitation and emission and an aggregation-induced emission molecule TPE through an oxygen ether bond. Compared with the IR-780 iodide,the probe of the invention has the significantly enhanced fluorescence intensity in an ethanol solution, shows a typical AIEE effect in a mixed solvent, can selectively generate photo-thermal killingeffects on various cancer cells at a concentration of 0.3 [mu]M, has no obvious killing effect on normal cells at a concentration of 0.3 [mu]M, and has remarkable killing selectivity. According to the invention, the probe is simple to prepare, has good light stability and good photo-thermal conversion capability, has excitation and emission in a near-infrared region, is less in background interference and damage to organisms, has diagnosis and treatment integrated function and is expected to be applied to clinical research.

The invention discloses a SiO2 gold nanorod (GNR) composite particle preparation method, which comprises the steps of preparing growth solution, preparing GNR, and preparing GNR-SiO2. The method disclosed by the invention achieves good repeatability, ideal near-infrared excitation window and ideal light-thermal conversion efficiency, and improves the application value of the GNR in the biological field.

The invention discloses preparation of a mitochondria targeting near-infrared fluorescent probe with an aggregation-induced emission effect. The fluorescent probe is composed of a hemicyanine part with near-infrared excitation and emission and a TPE part with aggregation-induced emission molecules. The probe has weak near-infrared fluorescence in a benign solvent, the fluorescence is gradually enhanced along with the addition of poor solvent water, and an obvious AIE effect is displayed; and along with the increase of the pH value, the fluorescence is also gradually enhanced, and the strong pHdependence is shown. The probe is simple to prepare, has good light stability and biocompatibility, is excited and emitted in a near-infrared region, has small biological light damage and small background interference, and opens up a new thought for development and application of near-infrared fluorescent probes with aggregation-induced emission.

The invention discloses a near-infrared waveband LED (light emitting diode) light source imaging system which comprises an optical system and a liquid crystal display system, wherein the optical system is used for developing a relatively deep vein; the optical system comprises a near-infrared light source and an image acquirer; the image acquirer is used for splitting reflection light into two paths to separate near-infrared light and visible light; the near-infrared reflection light contains information of the vein in a relatively deep position (greater than 3 mm); after image enhancement, an image of the vein can be subjected to image fusion with an image of the visible light, and the images can be displayed on a liquid crystal display screen and are referred by doctors when venipuncture is implemented. By using a dynamic frame-by-frame image fusion algorithm, a vein enhancement image obtained through near-infrared excitation can be overlapped with a common image in real time, and the whole venipuncture process can be completely displayed on the liquid crystal screen.

The invention discloses a turning light-splitting unit, and an endoscope optical imaging system and imaging method. The turning light-splitting unit is used for enabling the positions of imaging focal planes of light with different wavelengths to be consistent. The imaging system comprises a light signal transmitting unit, a light signal collection unit, and a camera unit. The light signal transmitting unit transmits visible light and near infrared exciting light to a to-be-detected tissue. The light signal collection unit collects the visible light reflected by the to-be-detected tissue and the near infrared fluorescence emitted by excitation. The camera unit receives two types of light collected by the light signal collection unit, and enables the positions of imaging focal planes of two types of light to be consistent. The imaging method comprises the steps: transmitting visible light and near infrared exciting light to the to-be-detected tissue; collecting the visible light reflected by the to-be-detected tissue and the near infrared fluorescence emitted by excitation; splitting two types of light, converging the two types of light, and enabling the positions of imaging focal planes of two types of light to be consistent. The system is simple in structure, is small in size, and is low in manufacture cost. The system can output color and fluorescence images at the same time, and is low in requirements for the operation capability of software.

The invention relates to a near-infrared fluorescent probe with obviously enhanced fluorescence intensity in the presence of glutathione thiotransferase. The structure of the fluorescent probe is shown in the specification, wherein R1, R2, R5, R6 and R7 are H, alkyl, aryl or substituent groups containing heteroatoms, and R3 and R4 are O or N atoms. The fluorescent probe can be used for near-infrared excitation and can emit near-infrared fluorescence to detect glutathione thiotransferase. The fluorescent probe is advantaged in that an optimized nitrobenzene structure is introduced into a fluorescent parent with near-infrared absorption and luminescence properties as an active center for recognition and reaction with glutathione thiotransferase, near-infrared fluorescence detection of the glutathione thiotransferase is realized by utilizing fluorescence intensity difference between reactant and a product, and penetrability and a signal-to-noise ratio are enhanced and practicability is improved as the emitted fluorescence further belongs to a near-infrared band.

The invention provides a near-infrared driven self-oxygen-supply compound, and a preparation method and application thereof. The compound comprises a photosensitizer, up-conversion nanoparticles and carrier blue-green algae; the photosensitizer and the up-conversion nanoparticles are loaded on the carrier blue-green algae; when near-infrared exciting light is applied for irradiation, the up-conversion nanoparticles can convert the near-infrared exciting light into multiple sections of exciting light with different wavelengths, wherein the visible light part is absorbed by blue-green algae, andgenerates a large amount of oxygen; and the exciting light at 650-700 nm enables the photosensitizer to generate singlet oxygen in the presence of oxygen. The compound has relatively high biologicalsafety to a body, has an obvious photodynamic therapy effect on malignant melanoma mice, solves the problem that photodynamic therapy is invalid to a tumour hypoxic region, provides a new scientific basis for radical treatment of tumours, and has important scientific significance.

The invention discloses a lateral flow test strip for serum marker luminescence detection based on near-infrared excitation and emission as well as a preparation method and an application method of the lateral flow test strip, which belongs to the field of immunological detection and aims to solve the technical problems that an existing nano material for biological detection has optical damage toorganisms and has high detection background signal interference. The lateral flow test strip consists of a polyvinyl chloride supporting back plate, a detection pad, a combination pad, a sample pad and an absorption pad, wherein the combination pad is a down-conversion [alpha]-NaLnF4:Nd,Yb@CaF2 / NaLnF4 nanocrystalline probe loaded with a combination antibody, and a down-conversion [alpha]-NaLnF4:Nd,Yb@CaF2 / NaLnF4 nanocrystalline probe combined with mouse IgG. The lateral flow test strip provided by the invention has a wide detection linear range and strong applicability, has low requirements onthe volume of a to-be-detected sample, short in required detection time, capable of realizing bedside detection and high in flexibility; and the lateral flow test strip does not need professional personnel to operate and can be used for medical detection.

The embodiment of the invention provides an optical molecular imaging navigation system. The system comprises a visible light source, a near infrared light source, an endoscope device, a transferringstructure, a beam splitting device, a camera device and an image processing device, wherein the visible light source provides visible light for collecting a background image, the near infrared light source provides near infrared light, the near infrared light is exciting light for generating fluorescent light, the endoscope device comprises an objective lens for converging and receiving hybrid beam of the visible light and the near infrared light, the hybrid beam is hybrid reflecting light which is generated after the visible light and the near infrared light irradiate a to-be-imaged object, the transferring structure converts the inverted hybrid beam received by the objective lens into anastigmatic hybrid beam and transmits the anastigmatic hybrid beam into the beam splitting device, thebeam splitting device splits the anastigmatic hybrid beam, the beam-split visible light is a beam of the background image of a background environment, the beam-split near infrared light is a beam of an object fluorescent image of the to-be-imaged object, the camera device receives the background image and the object fluorescent image from the beam splitting device, and the image processing deviceconducts fusing treatment on the background image and the object fluorescent image and outputs the fused image.

The invention discloses a compound I, a preparation method thereof and application of the compound I as a fluorescent probe. The compound I has near-infrared excitation / emission, the excitation wavelength ranges from 500 nm to 750 nm; and the compound is excited at a maximum absorption wavelength of 670 nm, and the emission wavelength is 700 to 810 nm. In addition, the compound I has an ultra-highsensitivity response to the PGP-1 enzyme. The detection limit for the PGP-1 enzyme at lambda ex / em=670 / 700 nm is 0.18 ng / mL. The compound I is used as a fluorescent probe to realize PGP-1 in vivo detection and fluorescence imaging for the first time. In vitro, cell and in vivo PGP-1 detection and fluorescence imaging can be realized, and all cells and tissues with inflammatory response and canceration can be assayed and imaged.

The invention relates to the technical field of new materials, in particular to a carbon dot-based room-temperature phosphorescent composite material suitable for near-infrared excitation as well as a preparation method, application and a using method of the carbon dot-based room-temperature phosphorescent composite material. The carbon dot-based room-temperature phosphorescent composite material is prepared from a NaYF4: Yb, Tm rare earth upconversion material and a carbon dot-based room-temperature phosphorescent material. The carbon dot-based room-temperature phosphorescent composite material can emit bright afterglow under near-infrared excitation, and has application in manufacturing of anti-counterfeiting products, sensing products, information encryption products, photoelectric device products or biological imaging products.

The invention discloses a photomagnetic nanoprobe with a core-point shell structure as well as a preparation method and application of the photomagnetic nanoprobe. The photomagnetic nanoprobe comprises a SiO2 core, Gd2O3 particles which are used as point shells, are distributed on the surface of the SiO2 core and are doped with metal ions, and target molecules which are hung on the surface of theSiO2 core, wherein the metal ions are Yb < 3 + > and Er < 3 + >, or Yb < 3 + >, Er < 3 + > and Li < + >. The photomagnetic nanoprobe with the core-point shell structure provided by the invention has high magnetic resonance relaxation enhancement performance, biocompatibility and structural stability, and can be applied to nuclear magnetic resonance enhanced imaging as a contrast agent; and meanwhile, the photomagnetic nanoprobe has excellent up-conversion photoluminescence capability, and is converted into red light and green light under near-infrared excitation, so that the photomagnetic nanoprobe has high-sensitivity multicolor optical imaging of tumor cells.

The invention discloses a bidirectional transition luminescent material and a preparation method thereof. Diazoacetate and alkene of which double bonds connected to an electrophilic group undergo 1,3-dipolar cycloaddition reaction to produce the bidirectional transition luminescent material. The bidirectional transition luminescent material is a polymer of which the side chain is connected to a pyrazoline group with fluorescence performances or a pyrazoline monomer contains other groups so that the material has special bidirectional transition luminescence performances. The bidirectional transition luminescent material can emit fluorescence with the same wavelength, can image in organisms under the actions of near infrared excitation and near ultraviolet excitation, can produce dual wavelength-excited fluorescence with intensity higher than that of fluorescence produced by single wavelength excitation and can further improve fluorescence imaging sharpness.

The present invention relates to a fluorescence imaging device and a test method using spectral detection, the imaging device comprising: an imaging unit and a spectrum detection part. The imaging unit is used for emitting visible light and near-infrared light to a specimen having a specific targeting fluorescent protein therein and converting the visible light and near-infrared light into a visible light image and a near-infrared light image; an area with the highest fluorescence brightness is selected as a reference area, an area with the fluorescence brightness weaker than that of the reference area by a certain degree is selected as an area to be detected, near-infrared excitation light of a spectrum detection part is adopted to irradiate the area to be detected, and a spectrograph isadopted to detect the peak wavelength of fluorescence emitted by the area to be detected. And the peak wavelength of the area is compared with the highest fluorescence brightness with the peak wavelength of the are to be detected, and the boundary region of the specific targeting fluorescent protein is determined. According to the fluorescence imaging device, the spectrograph is introduced, and the position and the boundary region of the specific targeting fluorescent protein are distinguished by accurately measuring the peak wavelength through the spectrograph by utilizing the characteristicsof the specific targeting fluorescent protein.