Fluorescence imaging device applying spectrum detection and testing method

Abstract

The present invention relates to a fluorescence imaging device and a test method using spectral detection, the imaging device comprising: an imaging unit and a spectrum detection part. The imaging unit is used for emitting visible light and near-infrared light to a specimen having a specific targeting fluorescent protein therein and converting the visible light and near-infrared light into a visible light image and a near-infrared light image; an area with the highest fluorescence brightness is selected as a reference area, an area with the fluorescence brightness weaker than that of the reference area by a certain degree is selected as an area to be detected, near-infrared excitation light of a spectrum detection part is adopted to irradiate the area to be detected, and a spectrograph isadopted to detect the peak wavelength of fluorescence emitted by the area to be detected. And the peak wavelength of the area is compared with the highest fluorescence brightness with the peak wavelength of the are to be detected, and the boundary region of the specific targeting fluorescent protein is determined. According to the fluorescence imaging device, the spectrograph is introduced, and the position and the boundary region of the specific targeting fluorescent protein are distinguished by accurately measuring the peak wavelength through the spectrograph by utilizing the characteristicsof the specific targeting fluorescent protein.

Description

technical field [0001] The invention relates to the field of imaging, in particular to a fluorescence imaging device and a testing method using spectrum detection. Background technique [0002] The optical molecular imaging method is applied to endoscopic imaging. By injecting specific protein-targeting fluorescent reagents and using two light sources, white light and excitation light, to illuminate the sample area, clear color images of the imaging area and reaction-specific targeting are simultaneously obtained. Fluorescent images of proteins. This method can perform in vivo imaging of specifically targeted protein fluorescent tissue at the molecular level of tumor cells, and has the advantages of low cost, high throughput, non-invasive, non-contact, non-ionizing radiation, high sensitivity, and high specificity. However, because some specific target protein sites are not easy to be found or are located in deep tissues, their positions and boundaries are difficult to accu...

AI Technical Summary

Problems solved by technology

However, because some specific target protein sites are not easy to be found or in deep tissues, its position and boundary are difficult to accurately determine

Images