54 results about "Immunoactivators" patented technology

The present invention discloses a kind of diazosulfide new compound structure and its application for promoting plant cell secondary metabolic product growth. Said invention selects and uses taxus chinensis cell as application system, and proceeds from structure of immunoactivating agent thiobiazole to design and synthesize new type plant cell inductive activating agent. The test results show that the capacity of said invented benzothiobiazole compound for promoting production of taxane is similar to or higher than that of existent methyl jasmonate.

The invention relates to penicillium protein elicitor EPTP and application thereof in improving plant disease resistance. The protein sequence of the EPTP protein elicitor is shown as SEQ ID NO.2, and the molecular weight is 19247 Da. EPTP induces significant increase of activity of phenylalanine ammonialyase, peroxidase and polyphenol oxidase, and when the elicitor induces tobacco anaphylactic reaction, an anaphylactic reaction marker gene hin1 is induced to express; it is indicated through semiquantitative RT-PCR results that EPTP can induce tobacco to obtain systemic acquired resistance, cause up-regulation of the tobacco blade resistance gene transcription level, and induce expression of resistance gene related to the salicylic acid path and the jasmonic acid path. After being applied, EPTP can improve resistance to viruses and pathogenic bacteria by plants, takes effects efficiently, does not pollute environment, reduces dosage of chemical pesticide and has wide application prospects as a plant immunity activator.

The invention relates to the technical field of medicines, and particularly relates to a peptide-modified multi-walled carbon nanotube carrier as well as a preparation method and application thereof. The invention provides the peptide-modified multi-walled carbon nanotube carrier which is formed by a nano-carrier MHR of a multi-walled carbon nanotube modified by short peptide composed of three arginines and six histidines. The nano-carrier provided by the invention can enhance solubility and dispersibility of carbon nanotubes in an aqueous solution, and reduce biological organism toxicity of the multi-walled carbon nanotubes; the prepared nano-carrier forms an MHR-CPG nano immune-activator by electrostatic adsorption of negatively charged immune-activator CpGDNA and the like; the nano-carrier provided by the invention can use MHR-CPG to stimulate the anti-tumor immune efficacy of the body, opens up a new treatment strategy for treatment of patients with prostate cancer, and also provides new theoretical basis and ideas for application of carbon nanotubes in drug carriers.

The present invention relates to the fields of immunology and molecular biology and describes compositions and methods for using proteins, peptides and nucleic acids related to the SARS CoV nucleocapsid protein and the spike glycoprotein. In particular, the present invention provides immunostimulatory preparations, prophylactic pharmaceutical preparations, diagnostic assays and kits for identifying and preventing SARS infections.

The invention provides an anti-tumor vaccine compound which is prepared by physically mixing ROS-rich cell microvesicle and immune activating agent. The invention provides a preparation method for theanti-tumor vaccine compound. The invention provides an anti-tumor vaccine compound injection. The invention provides an application of the anti-tumor vaccine compound. The anti-tumor vaccine compoundis applied to tumors, such as lung cancer, breast cancer, glioma, ovarian cancer, gastric cancer, colorectal cancer, osteogenic sarcoma, prostatic cancer, cervical cancer, malignant pleural effusionor melanoma. According to the invention, the problems of low immune activation efficiency, inaccuracy and few antigens of the present vaccine can be solved.

The invention relates to application of natural host defense peptides Alligatorin4 to preparing aquatic pathogenic bacterium resistant medicines, aquaculture animal immunity activators and aquaculture animal feed. The application has the advantages that as shown by results of research on Chinese mitten crab which is an experimental animal, immunity functions of the Chinese mitten crab can be enhanced by the natural host defense peptides Alligatorin4, the bacterial infection resistance of the Chinese mitten crab can be improved, and the survival rate of the Chinese mitten crab can be increased; the natural host defense peptides Alligatorin4 have broad application prospects in the field of aquaculture.

The present invention discloses a CpG-ODN having a specific immuno-stimulatory effect on pigs and an application thereof. A nucleotide sequence of the CpG-ODN is shown in SEQ ID NO:1. The CpG-ODN canimprove induction of innate immunity and specific humoral and cell-mediated immune responses of the pigs, and can be transferred into a plasmid vector pFAR4 to construct a recombinant plasmid pFAR4-CpG to prepare a preparation containing the recombinant plasmid pFAR4-CpG. The preparation can effectively activate natural immune functions of the pigs, also successfully replaces or partially replaces antibiotics as an immune activator, can also effectively activate acquired immune functions of the pigs, especially cellular immunity, can be used as an immune adjuvant to improve a protective effect of vaccines, also has little toxic and side effects on body, has great potential, can be widely used in live pig breeding industry, and is conducive to reducing breeding cost, reducing incidence ofthe pigs, and improving survival rate and breeding efficiency.

The invention relates to the technical field of biological nano-medicines, and specifically relates to a preparation method and application of a hybrid nano-tumor vaccine. The preparation method comprises the following steps: fully mixing a tumor cell lysis solution, a polypeptide hydrogel aqueous solution and a CpG-ODN aqueous solution, and dropwise adding a sodium chloride aqueous solution intothe mixed solution until the concentration of sodium chloride in the mixed solution is 0.9 wt%; and allowing the mixed solution stand to obtain the hybrid nano-tumor vaccine stably loaded with a tumorkilling polypeptide / CpG / lysis. According to the method disclosed by the invention, a polypeptide hydrogel nano vector is used as a bridge for loading a tumor cell antigen and an immunoactivator; theprepared tumor vaccine has a proper particle size, and can increase cell uptake and cytoplasm positioning of the tumor cell vaccine, activate inherent immunity and adaptive immunity of an organism toa greater extent, enhance an anti-tumor immune effect, and solve the problems that a common tumor antigen vaccine is easy to degrade, low in immunogenicity, low in immune response activation efficiency and the like. The hybrid gel vaccine can be applied to preparation of tumor vaccine drugs.

A vaccine having a good vaccination effect, which comprises an antigen; a peptide selected from the group consisting of a peptide having the amino acid sequence of SEQ ID NO: 1, a peptide having the amino acid sequence of SEQ ID NO: 2, and a peptide derived from a peptide having the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2; and an immune activator.

The invention discloses a novel composite nano preparation based on sonodynamic / immune combined therapy and a preparation method and application thereof, the novel composite nano preparation comprises a sound-sensitive agent, an immune activator, an active oxygen enhancing drug and liposome, the sound-sensitive agent and the active oxygen enhancing drug are entrapped in a liposome phospholipid bimolecular layer, and the immune activator is embedded on the lipidosome phospholipid bimolecular layer. The method comprises the following steps: (1) preparing a sound-sensitive agent, a cinnamyl aldehyde derivative, MPLA, a lecithin and DSPE-PEG5k into a solution; (2) evaporating out the solvent in the solution obtained in the step (1), and dispersing the drug-loaded lipid film in a buffer solution to obtain a drug-loaded liposome suspension; and (3) enabling the solution obtained in the step (2) to pass through a polycarbonate film to obtain the composite nano-liposome. The preparation can induce anti-tumor reaction, not only can prevent the development of in-situ solid tumors, but also can prevent the tumors from metastasis to far-end tissues. The composition is especially suitable for a combined treatment preparation of sonodynamic therapy and immunotherapy.

Provided is a modified oncolytic virus having a first heterologous polynucleotide encoding an immune checkpoint inhibitor and a second heterologous polynucleotide encoding an immune activator. Also provided is a pharmaceutical composition comprising the modified oncolytic virus and a method of treating a cancer comprising administering to a subject the modified oncolytic virus or the pharmaceutical composition.

Provided are methods and compositions for modulating an immune response or for treating a disease or condition in a subject, such as cancer, infection, autoimmune disease, allergy, and asthma. The methods involve systemically administering to a subject a particle comprising a surface and an interior, wherein the surface of the particle comprises an antigen, the interior of the particle comprises an immune modulating agent, and the subject is or has been primed to mount an antibody response to the antigen. The antibody response to the particle permits Fc receptor-bearing target cells to take up the particle, thereby delivering the immune modulating agent to the target cells and modulating an immune response of the subject. In various embodiments, the immune modulating agent can be selected from the group consisting of therapeutic agents, immune activators, and immune suppressors. In certain embodiments, the immune activator is a TLR agonist, e.g, a CpG oligodeoxynucleotide.

Cancer immunotherapy is enhanced by co-expression of cancer associated or tumor-specific (neo)epitopes with co-stimulatory molecules and / or other immune activators. Where desired, treatment may be enhanced by administration of a immune checkpoint inhibitor.

An immunogenic agent suitable for preventing, treating or immunizing against one or a plurality of different pathogen comprises an immunogenic agent which comprises one or a plurality of pathogen-derived proteins, fragments, variants or derivatives thereof displayed on a lipid vesicle and a carrier protein such as diptheria toxoid located in an intravesicular space. The immunogenic agent may be suitable for intranasal administration and may be capable of eliciting a mucosal immune response. The immunogenic agent may further comprise an activator of innate immunity such as trehalose-6,6'-dibehenate and / or a bile salt such as sodium deoxycholate. The one or plurality of pathogens may be group A streptococcus, viruses or hookworms.

The present invention discloses a kind of diazosulfide new compound structure and its application for promoting plant cell secondary metabolic product growth. Said invention selects and uses taxus chinensis cell as application system, and proceeds from structure of immunoactivating agent thiobiazole to design and synthesize new type plant cell inductive activating agent. The test results show that the capacity of said invented benzothiobiazole compound for promoting production of taxane is similar to or higher than that of existent methyl jasmonate.