54 results about "Immunoactivators" patented technology

The present invention discloses a kind of diazosulfide new compound structure and its application for promoting plant cell secondary metabolic product growth. Said invention selects and uses taxus chinensis cell as application system, and proceeds from structure of immunoactivating agent thiobiazole to design and synthesize new type plant cell inductive activating agent. The test results show that the capacity of said invented benzothiobiazole compound for promoting production of taxane is similar to or higher than that of existent methyl jasmonate.

The present invention is directed to a method of activating or augmenting an immune system of a mammal. The method comprises the step of administering a isothiocyanate (ITC) based agent, such as, N-acetylcysteine conjugate of phenethyl isothiocyanate (PEITC-NAC) or phenethyl isothiocyanate (PEITC), to a mammal in an amount sufficient to activate or augment the immune response in the mammal. The invention is further directed to a method of activating an antigen-specific immune response by administering to a subject an ITC based agent to in an amount sufficient to activate or augment the response of a B cell system or innate immunity to an antigen.

The invention relates to penicillium protein elicitor EPTP and application thereof in improving plant disease resistance. The protein sequence of the EPTP protein elicitor is shown as SEQ ID NO.2, and the molecular weight is 19247 Da. EPTP induces significant increase of activity of phenylalanine ammonialyase, peroxidase and polyphenol oxidase, and when the elicitor induces tobacco anaphylactic reaction, an anaphylactic reaction marker gene hin1 is induced to express; it is indicated through semiquantitative RT-PCR results that EPTP can induce tobacco to obtain systemic acquired resistance, cause up-regulation of the tobacco blade resistance gene transcription level, and induce expression of resistance gene related to the salicylic acid path and the jasmonic acid path. After being applied, EPTP can improve resistance to viruses and pathogenic bacteria by plants, takes effects efficiently, does not pollute environment, reduces dosage of chemical pesticide and has wide application prospects as a plant immunity activator.

The invention relates to the technical field of medicines, and particularly relates to a peptide-modified multi-walled carbon nanotube carrier as well as a preparation method and application thereof. The invention provides the peptide-modified multi-walled carbon nanotube carrier which is formed by a nano-carrier MHR of a multi-walled carbon nanotube modified by short peptide composed of three arginines and six histidines. The nano-carrier provided by the invention can enhance solubility and dispersibility of carbon nanotubes in an aqueous solution, and reduce biological organism toxicity of the multi-walled carbon nanotubes; the prepared nano-carrier forms an MHR-CPG nano immune-activator by electrostatic adsorption of negatively charged immune-activator CpGDNA and the like; the nano-carrier provided by the invention can use MHR-CPG to stimulate the anti-tumor immune efficacy of the body, opens up a new treatment strategy for treatment of patients with prostate cancer, and also provides new theoretical basis and ideas for application of carbon nanotubes in drug carriers.

The invention provides an anti-tumor vaccine compound which is prepared by physically mixing ROS-rich cell microvesicle and immune activating agent. The invention provides a preparation method for theanti-tumor vaccine compound. The invention provides an anti-tumor vaccine compound injection. The invention provides an application of the anti-tumor vaccine compound. The anti-tumor vaccine compoundis applied to tumors, such as lung cancer, breast cancer, glioma, ovarian cancer, gastric cancer, colorectal cancer, osteogenic sarcoma, prostatic cancer, cervical cancer, malignant pleural effusionor melanoma. According to the invention, the problems of low immune activation efficiency, inaccuracy and few antigens of the present vaccine can be solved.

The invention relates to application of natural host defense peptides Alligatorin4 to preparing aquatic pathogenic bacterium resistant medicines, aquaculture animal immunity activators and aquaculture animal feed. The application has the advantages that as shown by results of research on Chinese mitten crab which is an experimental animal, immunity functions of the Chinese mitten crab can be enhanced by the natural host defense peptides Alligatorin4, the bacterial infection resistance of the Chinese mitten crab can be improved, and the survival rate of the Chinese mitten crab can be increased; the natural host defense peptides Alligatorin4 have broad application prospects in the field of aquaculture.

The invention provides a pesticide composition containing one of fenoxanil as effective ingredient, probenazole as plant immunoactivator and rabcide as protective bactericide, as well as the rest auxiliary ingredients which are allowed and acceptable in pesticide and, wherein the mass percent of the effective ingredient in the composition is 5%-80%. The pesticide composition according to the invention is mainly used for the prevention and the treatment of paddy rice diseases, and is favorably effective to rice blast especially.

The invention provides fructan-high-yielding bacillus amyloliquefaciens and application thereof. The preservation number of B. amyloliquefaciens 66 at China General Microbiological Culture CollectionCenter is CGMCC No. 14363. The strain as a feed probiotic for producing functional fructan is used as a good immune activator, can effectively improve the activity of feeding biological interferon andmacrophages, and stimulate the immune function of a biological organism by producing non-specific immunomodulatory factors, and enhance the immunity and disease resistance of the organism. The straincan also produce amylase, protease, cellulase, hemicellulase and other digestive enzymes in the growth process to enhance the nutritional metabolism in the host. At the same time, the yield of extracellular fructan which is fermentatively produced by B. amyloliquefaciens is up to 100g / L. Therefore, the strain amyloliquefaciens 66 disclosed in the invention not only has good application potentialin the field of feed additives, but also shows great development potential and market value in the fields of biomedicine and functional food.

The present invention discloses a CpG-ODN having a specific immuno-stimulatory effect on pigs and an application thereof. A nucleotide sequence of the CpG-ODN is shown in SEQ ID NO:1. The CpG-ODN canimprove induction of innate immunity and specific humoral and cell-mediated immune responses of the pigs, and can be transferred into a plasmid vector pFAR4 to construct a recombinant plasmid pFAR4-CpG to prepare a preparation containing the recombinant plasmid pFAR4-CpG. The preparation can effectively activate natural immune functions of the pigs, also successfully replaces or partially replaces antibiotics as an immune activator, can also effectively activate acquired immune functions of the pigs, especially cellular immunity, can be used as an immune adjuvant to improve a protective effect of vaccines, also has little toxic and side effects on body, has great potential, can be widely used in live pig breeding industry, and is conducive to reducing breeding cost, reducing incidence ofthe pigs, and improving survival rate and breeding efficiency.

The invention relates to the technical field of biological nano-medicines, and specifically relates to a preparation method and application of a hybrid nano-tumor vaccine. The preparation method comprises the following steps: fully mixing a tumor cell lysis solution, a polypeptide hydrogel aqueous solution and a CpG-ODN aqueous solution, and dropwise adding a sodium chloride aqueous solution intothe mixed solution until the concentration of sodium chloride in the mixed solution is 0.9 wt%; and allowing the mixed solution stand to obtain the hybrid nano-tumor vaccine stably loaded with a tumorkilling polypeptide / CpG / lysis. According to the method disclosed by the invention, a polypeptide hydrogel nano vector is used as a bridge for loading a tumor cell antigen and an immunoactivator; theprepared tumor vaccine has a proper particle size, and can increase cell uptake and cytoplasm positioning of the tumor cell vaccine, activate inherent immunity and adaptive immunity of an organism toa greater extent, enhance an anti-tumor immune effect, and solve the problems that a common tumor antigen vaccine is easy to degrade, low in immunogenicity, low in immune response activation efficiency and the like. The hybrid gel vaccine can be applied to preparation of tumor vaccine drugs.

A vaccine having a good vaccination effect, which comprises an antigen; a peptide selected from the group consisting of a peptide having the amino acid sequence of SEQ ID NO: 1, a peptide having the amino acid sequence of SEQ ID NO: 2, and a peptide derived from a peptide having the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2; and an immune activator.

The present invention is based on the finding that combined use of a multispecific antibody comprising: (1) a cancer-specific antigen-binding domain, (2) a CD3-binding domain, and (3) a domain comprising an Fc region having decreased Fcγ receptor-binding activity with a tumor necrosis factor (TNF) receptor superfamily agonist antibody can reduce side effects such as liver injury observed when the agonist antibody is prescribed alone, and achieve effective therapeutic effects.

The invention discloses a novel composite nano preparation based on sonodynamic / immune combined therapy and a preparation method and application thereof, the novel composite nano preparation comprises a sound-sensitive agent, an immune activator, an active oxygen enhancing drug and liposome, the sound-sensitive agent and the active oxygen enhancing drug are entrapped in a liposome phospholipid bimolecular layer, and the immune activator is embedded on the lipidosome phospholipid bimolecular layer. The method comprises the following steps: (1) preparing a sound-sensitive agent, a cinnamyl aldehyde derivative, MPLA, a lecithin and DSPE-PEG5k into a solution; (2) evaporating out the solvent in the solution obtained in the step (1), and dispersing the drug-loaded lipid film in a buffer solution to obtain a drug-loaded liposome suspension; and (3) enabling the solution obtained in the step (2) to pass through a polycarbonate film to obtain the composite nano-liposome. The preparation can induce anti-tumor reaction, not only can prevent the development of in-situ solid tumors, but also can prevent the tumors from metastasis to far-end tissues. The composition is especially suitable for a combined treatment preparation of sonodynamic therapy and immunotherapy.

The invention discloses a formula of a biological feed additive capable of resisting diseases and promoting growth. The formula is simple in process and good in using effect, and includes the following raw materials in percentage: 90% of feedstuff, 5% of bacillus, 3% of yeast and 2% of lactic acid bacteria. The preparation process includes the following steps: preparing materials, mixing, fermenting and packaging. The core technology of the invention is that three types of bacteria, namely the bacillus, the yeast and the lactic acid bacteria, are added, wherein the lactic acid bacteria adheres to the stomach and intestine of animals for promoting digestion; yeast only adheres to the intestine, is free of infection by any other bacteria, can infect other bacteria and resist harmful bacteria, and promotes intestinal absorption; and in addition, zymosan, a metabolite generated with yeast, is contained, and as a plant polysaccharide, the zymosan is an immune activator, and can activate the immunity of animal bodies, improve disease resistant capabilities of animals and reduce the incidence; as growth inhibiting bacteria, the bacillus can kill and inhibit various harmful bacteria and pathogens in the animal bodies and helps the animals break down and digest food.

The invention relates to a fruit tree weak parasitic disease green prevention and control method which includes the steps: improving soil environments by bio-organic fertilizers and mineral ecologicalfertilizers, and spraying induced immune activators to activate disease-resistant mechanisms of plants; spraying plant source sterilizing agents in an auxiliary manner before weak parasitic diseaseshappen. According to the method, improvement of soil, induced immune activation and the plant source sterilizing agents are combined, immunity and disease resistance of walnuts are improved, canker ofthe walnuts is effectively controlled, production capability of fruit trees is improved, a fruit bearing period is prolonged, and the method is of great significance for guarantee of production benefits of walnut framers and facilitating of development of rural economy walnut industry.

[PROBLEMS] To provide a fraction containing the active ingredient contained in plant worms. To provide a means efficacious in immunopotentiation, an effective means of preventing or treating cancer and a composition useful in producing health foods. [MEANS FOR SOLVING PROBLEMS] It is intended to provide a precipitation fraction obtained by extracting plant worms mycelium with hot water and adding ethanol to the thus obtained extract. It is also intended to provide a composition for oral intake which contains the above precipitation fraction. It is further intended to provide foods, drinks, immunopotentiators, anticancer agents and so on containing the above composition for oral intake which are characterized by having an anticancer effect, an effect of preventing cancer or an immunopotentiation effect. It is furthermore intended to provide a method of effectively producing the above-described composition for oral intake.

An animal immunopotentiator, cytokine adjuvant-chicken Newcastle disease vaccine (cc strain) is prepared by coordination of the cytokine (thymopeptide or transfer factor) with low-virulent immunoactivator of Newcastle disease.

Cancer immunotherapy is enhanced by co-expression of cancer associated or tumor-specific (neo)epitopes with co-stimulatory molecules and / or other immune activators. Where desired, treatment may be enhanced by administration of a immune checkpoint inhibitor.

The invention relates to application of natural host defense peptide Alligatorin5 to preparation of aquatic pathogenic bacterium resisting medicine. The invention also discloses application of the natural host defense peptide Alligatorin5 to preparation of an aquaculture animal immune activating agent. The invention further discloses application of the natural host defense peptide Alligatorin5 to preparation of aquaculture animal feed. The results of study using hairy crabs as experimental animals show that the Alligatorin5 can enhance the immune function of the hairy crabs; the immunity of the hairy crabs on bacterial infection is improved; the survival rate of the hairy crabs is improved. The Alligatorin5 has wide application prospects in the field of aquaculture.

Provided are methods and compositions for modulating an immune response or for treating a disease or condition in a subject, such as cancer, infection, autoimmune disease, allergy, and asthma. The methods involve systemically administering to a subject a particle comprising a surface and an interior, wherein the surface of the particle comprises an antigen, the interior of the particle comprises an immune modulating agent, and the subject is or has been primed to mount an antibody response to the antigen. The antibody response to the particle permits Fc receptor-bearing target cells to take up the particle, thereby delivering the immune modulating agent to the target cells and modulating an immune response of the subject. In various embodiments, the immune modulating agent can be selected from the group consisting of therapeutic agents, immune activators, and immune suppressors. In certain embodiments, the immune activator is a TLR agonist, e.g, a CpG oligodeoxynucleotide.

The invention belongs to the technical field of medicines, and in particular relates to applications of polyetherimide in preparing medicines for preventing influenza virus infection. The influenza virus infection animal model and the animal protection experiment developed on the influenza virus infection animal model prove that after nasal-passage administration of polyetherimide in advance, the mice can resist the infection of H1N1 with the lethal dose, which proves that polyetherimide has the function of preventing influenza virus infection; the research result of the action mechanism shows that polyetherimide has the obvious advantages compared with other immunity activators, can induce antiviral factors, and has the small induction effect for IL-17 which can aggravate the conditions of influenza virus. Polyetherimide can be further developed to be prepared into the medicines for preventing influenza virus infection, and has the wide application prospect.

The invention relates to application of a DNA enzyme in the pharmaceutical field and a preparation method thereof. The DNA enzyme which is an immune activator extracted from a micrococcus catarrhalis is applied into preparing medicines, thus curing or preventing various diseases. When the DNA enzyme is prepared, a purely cultivated micrococcus catarrhalis is inoculated on a culture medium for cultivation; a lawn is washed and diluted to liquid containing mycelium; the effective component DNA enzyme which is hotly dipped is cooled and then is sucked out the supernatant fluid after being centrifugally separated; and then the supernatant fluid is processed by high pressure and is cooled to room temperature, in this way, the DNA enzyme which is an immune activator extracted from a micrococcus catarrhalis is obtained. The invention applies the immune activator DNA medicines into curing diseases for the first time, aiming at remedying the errors of gene orders and repairing the damages through activating gene origins, thus curing and preventing diseases and improving nonspecific immunity, and realizing the purpose of curing genes in the real sense. The preparation method is simple and practicable, without any toxic and side effects.

The invention discloses an application of hypholomine B in the preparation of an immune activator, the hypholomine Bcan effectively promote the secretion of INF-gamma and other cytokines by macrophages, INF-gamma and other cytokines have strong antiviral, immunoregulatory and antitumor activities; that hypholomin B can effectively enhance the phagocytosis activity of the macrophages on Escherichiacoli and kill tumor cells is confirmed. The hypholomethine B can promote the proliferation of RAW264.7 cells and promote the phagocytic activity of the RAW264.7 cells, and the hypholomethine B can promote the killing activity of the macrophages to tumor cells in a dose-dependent manner. Therefore, the new application of the hypholomin B in the aspect of immunoregulation can be used as a new indexof the effective components of phellinus linteus, and also can provide a thought for further scientific utilization of the phellinus linteus, thereby effectively improving the economic benefit of thephellinus linteus.

An immunogenic agent suitable for preventing, treating or immunizing against one or a plurality of different pathogen comprises an immunogenic agent which comprises one or a plurality of pathogen-derived proteins, fragments, variants or derivatives thereof displayed on a lipid vesicle and a carrier protein such as diptheria toxoid located in an intravesicular space. The immunogenic agent may be suitable for intranasal administration and may be capable of eliciting a mucosal immune response. The immunogenic agent may further comprise an activator of innate immunity such as trehalose-6,6'-dibehenate and / or a bile salt such as sodium deoxycholate. The one or plurality of pathogens may be group A streptococcus, viruses or hookworms.

The invention discloses a chicken specificity immune activator CpG-ODN and an application thereof, and a nucleotide sequence of the CpG-ODN is shown as SEQ ID NO: 1. The CpG-ODN can enhance an induction effect of innate immunity and specific humoral and cell-mediated immune responses of chickens, and can be transferred into a plasmid vector pVAX1 to construct a recombinant plasmid pVAX1-CpG, and apreparation containing the recombinant plasmid pVAX1-CpG is prepared. The preparation can effectively activate a natural immune function of the chickens, and also successfully replace or partially replace antibiotics as an immune activator; the preparation can also effectively activate an acquired immune function of the chickens, especially cellular immunity, and can be used as an immune adjuvantto enhance a protective effect of vaccines; and the preparation has little toxic and side effects on a body, has great potential, can be widely applied to a breeding industry of raw chickens, is beneficial to reduction of breeding cost, enables incidence of a disease of the raw chickens to be reduced, and improves the survival rate and the breeding efficiency of the chickens.

The present invention discloses a kind of diazosulfide new compound structure and its application for promoting plant cell secondary metabolic product growth. Said invention selects and uses taxus chinensis cell as application system, and proceeds from structure of immunoactivating agent thiobiazole to design and synthesize new type plant cell inductive activating agent. The test results show that the capacity of said invented benzothiobiazole compound for promoting production of taxane is similar to or higher than that of existent methyl jasmonate.