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Fructan-high-yielding bacillus amyloliquefaciens and application thereof

A technology of amylolytic spores and fructans, applied in the direction of application, bacteria, and microorganism-based methods, etc., to achieve good probiotic effects, enhance the body's immunity and disease resistance, and simple fermentation and cultivation conditions

Inactive Publication Date: 2018-01-16
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Exopolysaccharides derived from microorganisms have various biological functions such as anti-tumor, anti-diabetes, lowering blood lipids, and improving immunity. However, the current yield has become the main factor restricting its application.
For example, exopolysaccharides from Bacillus amyloliquefaciens from winter wheat can inhibit the growth activity of gastric cancer cells MC-4 and SGC-7901; polysaccharides from Bacillus amyloliquefaciens LPL061 have immunomodulatory effects, but the fermentation yield is only 4.46 g / L; Cellulomonas sp.GJT07 fermentation optimization results in a glycan yield of 15g / L

Method used

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  • Fructan-high-yielding bacillus amyloliquefaciens and application thereof
  • Fructan-high-yielding bacillus amyloliquefaciens and application thereof
  • Fructan-high-yielding bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 B.amyloliquefaciens 66 liquid fermentation (1T fermentation tank)

[0024] 1. Preparation of primary seed liquid: Inoculate the bacterial liquid stored in the glycerol tube into 20mL LB medium at an inoculum amount of 2‰, and incubate with shaking at 37°C and 220rpm for 10-12h.

[0025] 2. Preparation of the secondary seed solution: Inoculate the primary seed solution in 200mL LB medium according to the inoculum amount of 2%, and culture with shaking at 37°C and 200rmp for 10-12h.

[0026] 3. Preparation of the tertiary seed liquid: inoculate the secondary seed liquid into 5L fermentation medium at 37°C and pH 7.0 and cultivate until the DO rises.

[0027] 4. Preparation of 100L seed liquid: Inoculate the third-grade seed liquid into 100L fermentation medium for cultivation at 37°C and pH 7.0. The tank pressure is 0.05MPa, the ventilation is 150-300L / m, and the initial rotation speed is 150rpm. Set the speed to dissolve oxygen (40%) in series, and the maxi...

Embodiment 2

[0032] Example 2 Detection of application characteristics of strain B.amyloliquefaciens 66

[0033] Table 1 The performance of B.amyloliquefaciens 66 and its comparison with commercial products

[0034]

[0035]

[0036]

[0037] Note: ★ corresponds to relative enzyme activity, the more ★, the higher the enzyme activity.

[0038] It can be seen that compared with the commercially available probiotics, the B. amyloliquefaciens 66 strain has many advantages such as high number of viable bacteria, strong strain performance, and low production cost.

Embodiment 3

[0039] Example 3 Effect of B.amyloliquefaciens 66 liquid bacterial agent on sow reproduction and litter performance

[0040] Select 48 Landrace lactating sows with close parity and good health, and divide them into two groups on average, 24 in each group. The experiment lasts from 30 days before farrowing to the end of weaning when the suckling piglets are 22 days old. The control group is basal diet. The group was supplemented with 1ml of probiotics daily. The results are shown in the table below:

[0041] Table 2 The effect of B.amyloliquefaciens 66 liquid bacterial agent on the reproduction and rearing performance of sows

[0042]

[0043] From the above results, it can be seen that compared with the control group, the sows fed B.amyloliquefaciens 66 liquid bacterial preparation produced a relatively higher average number of healthy litters, a relatively lower rate of weak litters and stillbirths, and an average litter weight increased by 28.8%, indicating that the p...

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Abstract

The invention provides fructan-high-yielding bacillus amyloliquefaciens and application thereof. The preservation number of B. amyloliquefaciens 66 at China General Microbiological Culture CollectionCenter is CGMCC No. 14363. The strain as a feed probiotic for producing functional fructan is used as a good immune activator, can effectively improve the activity of feeding biological interferon andmacrophages, and stimulate the immune function of a biological organism by producing non-specific immunomodulatory factors, and enhance the immunity and disease resistance of the organism. The straincan also produce amylase, protease, cellulase, hemicellulase and other digestive enzymes in the growth process to enhance the nutritional metabolism in the host. At the same time, the yield of extracellular fructan which is fermentatively produced by B. amyloliquefaciens is up to 100g / L. Therefore, the strain amyloliquefaciens 66 disclosed in the invention not only has good application potentialin the field of feed additives, but also shows great development potential and market value in the fields of biomedicine and functional food.

Description

technical field [0001] The invention belongs to the field of feed additives and biotechnology, and relates to a strain of bacillus amyloliquefaciens with high fructan yield and its application. Background technique [0002] Bacillus inoculum is a probiotic preparation widely used in animal husbandry. Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) belongs to Gram-positive, aerobic rod-shaped bacteria and is generally recognized as safe (Generally Recognized As Safe, GRAS) strains. This strain is widely distributed in nature, and it produces many types of secondary metabolites, strong antibacterial activity, wide antibacterial spectrum, and has functional characteristics such as activating immunity and promoting growth, showing a good effect of probiotics for feeding. application potential. However, although most of the probiotics on the market currently have complete labeling functions, they are not clear about their effectiveness. Up to now, the quality of product...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04A23K10/18C12R1/07
Inventor 宋诙郑宏臣甄杰郑雯谭明
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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