90results about How to "Good probiotic properties" patented technology

The present invention discloses a fruit and vegetable probiotic tablet and a preparation method thereof. The fruit and vegetable probiotic tablet uses probiotic powders such as lactobacillus plantarum powder as the main raw material, and the preparation method scientifically mixes modified dietary fibers, fruit and vegetable powder, oligosaccharides, plant extracts, protein powder, tea leaf extracts and traditional Chinese medicine extracts and etc., thus improves the content of soluble celluloses which are of real significance for probiotic flora, enhances the physiological activity of celluloses, thereby increases the species of intestinal probiotic flora as well as significantly enhances the colonization ability and time of endogenous and exogenous probiotics in the human intestinal tracts, effectively inhibits the growth and reproduction of harmful intestinal bacteria, especially gram-negative bacteria, and fully regulates the composition of the intestinal probiotic flora. The prepared fruit and vegetable probiotic tablet has a high biological activity, a long human intestinal colonization time, and a significant weight loss effect, and is suitable for a wide range of people.

The invention relates to Bacteroides fragilis and applications thereof, particularly to Bacteroides fragilis ZY-312 having the preservation number of CGMCC No.10685, and applications of the Bacteroides fragilis ZY-312 in preparation of medicines, pharmaceutical compositions, foods, health products and food additives for prevention and / or treatment of antibiotic-associated diarrhea, wherein the Bacteroides fragilis ZY-312 is preserved in the China general microbiological culture collection center on April 2, 2015, has the preservation number of CGMCC No.10685, does not contain enterotoxin gene bft, has characteristics of significantly-improved cholate resistance and significantly-improved gastric acid resistance compared to the existing Bacteroides fragilis, and can effectively prevent and / or treat antibiotic-associated diarrhea.

The invention discloses a nutritional health food with the function of improving gastrointestinal function and a preparation method thereof. The nutritional health food can be used for regulating the intestinal flora to improve the gastrointestinal function from the aspects of increasing the probiotics type and quantity, increasing the probiotics nutrient substance, inhibiting the growth of harmful bacteria and enhancing the immunity of human body. According to the preparation method, probiotics powder such as lactobacillus plantarum is used as the main raw material, and edible fungi extracts and modified dietary fibers are scientifically compounded; saussurea involucrata culture extracts which are capable of remarkably enhancing the immunity of human body, resisting inflammatory and easing pain, and have the functions of oxidation resistance, radiation resistance and fatigue resistance are used, so that while the intestinal probiotics flora types is increased, the fix planting capacity of the endogenous probiotics and exogenous probiotics in the intestinal tracts of human body is remarkably enhanced, and fix planting time of the endogenous probiotics and exogenous probiotics in the intestinal tracts of human body is prolonged remarkably, the growth and reproduction of the harmful bacteria, particularly the Gram negative bacteria, in the intestinal tracts are effectively inhibited, the forming of the probiotic floras in the intestinal tracts is fully regulated and the nutritional health food which is good in probiotic property, capable of remarkably enhancing the immunity of human body and has the effect of improving the gastrointestinal function is prepared.

The invention discloses a health food capable of improving intestinal floras and a preparation method of the health food. The intestinal floras are adjusted on a plurality of aspects of increasing the probiotics variety and quantity, adding probiotic nutrient substances, inhibiting the harmful bacterial growth and increasing the human immunity, so that the gastrointestinal functions are improved. With probiotic powder such as lactobacillus plantarum as a main raw material, an edible mushroom extract is scientifically compounded, and dietary fibers are modified, so that the human immunity can be significantly improved; a saussurea involucrate culture extract has the efficacies of resisting inflammation, relieving pain, and resisting oxidation, radiation and fatigue; the variety of the intestinal beneficial bacteria floras is increased; the field planting capacity of endogenous and exogenous probiotics in human intestinal tracts is significantly enhanced and the field planting time of endogenous and exogenous probiotics in human intestinal tracts is significantly prolonged; the growth and the reproduction of intestinal harmful bacteria, especially gram negative bacteria, are effectively inhibited; the components of the intestinal beneficial bacteria floras are fully adjusted; and the health food which is high in probiotic property, and is capable of significantly improving the human immunity and improving the intestinal floras is prepared.

The invention discloses a probiotic-containing health food and a preparation method thereof, wherein intestinal florae are adjusted in many aspects of increasing the types and quantity of probiotics, increasing the nutrient substances of the probiotics, inhibiting the growth of harmful fungi, enhancing human immunity and the like, so as to improve gastrointestinal functions. Probiotic powders of lactobacillus plantarum and the like are taken as main raw materials, and edible fungi extract, modified dietary fibres, a saussurea involucrata culture extract capable of remarkably enhancing the human immunity, resisting inflammation and easing pain and having anti-oxidization, anti-radiation, anti-fatigue functions and the like are scientifically compounded, and then the planting capacity and the planting time of endogenous and exogenous probiotics in human intestinal tracts are remarkably improved and prolonged, the growth and reproduction of intestinal harmful fungi, especially, Gram-negative bacterium, are effectively inhibited, and the composition of intestinal probiotic florae is adequately regulated while the types of the intestinal probiotic florae are increased; therefore, a health food with high probiotic property, capable of remarkably enhancing human immunity and containing probiotics is prepared.

The invention discloses weight-losing probiotic tablets and a preparation method thereof. According to the weight-losing probiotic tablets and the preparation method thereof, probiotic powder such as lactobacillus plantarum is taken as a main raw material, and modified dietary fibres, fruit and vegetable powder, oligosaccharides, plant extract, protein powder, tea leaf extract, Chinese herb extracts and the like are scientifically compounded, thus increasing the content of soluble celluloses with real significance in probiotic groups, and enhancing the physiological activity of the celluloses, and then remarkably improving the planting capacity and the planting time of endogenous and exogenous probiotics in human intestinal tracts, effectively suppressing the growth and the reproduction of intestinal harmful bacteria, in particular, Gram negative bacteria, and adequately regulating the composition of the intestinal probiotic groups while increasing the types of the intestinal probiotic groups; the prepared weight-losing probiotic tablets are high in bioactivity, long in planting time in the human intestinal tracts, remarkable in weight-losing effect, and wide in the range of appropriate crowds.

The invention discloses lactobacillus plantarum with oxidation resistance and preservation number of CGMCC15780, and application of the lactobacillus plantarum. The lactobacillus plantarum is a lactobacillus strain with high oxidation resistance, which is screened from traditional fermented yak yogurt and silage in the Qinghai-Tibet Plateau. In vivo and in vitro tests prove that the lactobacillusplantarum has relatively good oxidation resistance, and can be used for effectively promoting increase of the activity of antioxidative enzyme in a body. The lactobacillus plantarum having relativelygood hydrophobic performance can increase the opportunity of adhering to the intestinal walls and achieving the probiotic effect, does not have toxic or side effect, and can be used as health-protecting foods or medicines to enhance the body immunity, relieve senescence, beautify, regulate intestinal flora and the like.

The invention discloses a solid-state fermentation method for bacillus natto-saccharomycete composite viable bacteria preparation with rice bran as a matrix, which includes the following steps: fermentation medium preparation: rice bran and soya bean meal are chosen as main materials, and are weighed according to a fermentation medium formula, the components of the medium are 85 to 95 percent by weight of the rice bran, 2 to 10 percent by weight of the soya bean meal, 1 to 5 percent by weight of glucose and the balance of water, the materials are kept sufficiently moist, and are not agglomerated, the pH value of the materials is regulated to 6.0 to 6.5 by NaOH solution, and the materials are sterilized for later use; solid-state fermentation: as for the inoculation proportion of the fermentation strain, the weight ratio of bacillus natto seed fermentation broth to saccharomyces cerevisiae seed fermentation broth is (5 to 7):(5 to 3), the inoculation proportion is 5 to 10 percent by weight, the solid-state fermentation time is 4 to 6 days, and thereby the composite viable bacteria preparation is obtained; and composite viable bacteria preparation drying and packaging are carried out. The composite viable bacteria preparation has the characteristics of high viable count, low cost, simple process, little investment, low energy consumption, no waste water, little environment pollution, convenient post-processing and the like.

The invention discloses a health food for resisting fatigue and improving gastrointestinal functions and a preparation method of the health food. Intestinal floras are adjusted on a plurality of aspects of increasing the probiotics variety and quantity, adding probiotic nutrient substances, inhibiting the harmful bacterial growth and increasing the human immunity, so that the gastrointestinal functions are improved. With probiotic powder such as lactobacillus plantarum as a main raw material, an edible mushroom extract is scientifically compounded, and dietary fibers are modified, so that the human immunity can be significantly improved; a saussurea involucrate culture extract has the efficacies of resisting inflammation, relieving pain, and resisting oxidation, radiation and fatigue; the variety of the intestinal beneficial bacteria floras is increased; the field planting capacity and the field planting time of endogenous and exogenous probiotics in human intestinal tracts are significantly enhanced; the growth and the reproduction of the intestinal harmful bacteria, especially gram negative bacteria, are effectively inhibited; the components of the intestinal beneficial bacteria flora are fully adjusted; and the health food which is high in probiotic property, and is capable of significantly improving the human immunity, resisting the fatigue and improving the gastrointestinal functions is prepared.

The invention provides a composition for adjusting intestinal microecology of a human body. The composition comprises stachyose and galactooligosaccharides as active ingredients. The composition provided by the invention can be used for effectively adjusting the intestinal bacteria community function.

The invention relates to a preparation method of a health beverage containing dioscorease rhizome extract and bifidobacterium bifidum and the prepared beverage, belonging to the technical field of foods and beverages. The preparation method comprises the steps of sterilizing fermentation culture solution of which the main raw materials include Chinese yam extract, xylooligosaccharide, soybean polysaccharide, soy peptone, skimmed milk, calcium lactate, compound concentrate Goji and the like according to specific conditions, inoculating lactobacillus plantarum solution and bifidobacterium bifidum solution sequentially, fermenting to obtain fermented milk base material in an anaerobic fermentation tank under certain tank pressure, filling or blending filling to obtain the health beverage containing dioscorease rhizome extract and bifidobacterium bifidum. The health beverage containing dioscorease rhizome extract and bifidobacterium bifidum has high viable organism content, short fermentation time and high viable organism survival rate, and the bifidobacterium bifidum has good fermentation property, acid resistance and gastric acid resistance, and has good probiotic effect.

The invention discloses a Lactobacillus plantarum strain BX62 with antioxidant ability. A preservation number is CGMCC15779. And the application of the strain is also included in the invention. The Lactobacillus plantarum strain BX62 is isolate from that traditional fermented yak yoghurt of the Qinghai-Tibet plateau, and is prove to be capable of remarkably improving the T-AOC, GSH-Px, T-SOD activity, and decrease the content of MDA in mice serum. The strain has good antioxidant ability and can effectively promote the activity of antioxidant enzymes in vivo. The hydrophobic ability of the strain increases the chance for it to adhere to the intestinal wall and play a probiotic role. The strain has no toxic and side effects and can be used in the preparation of fermented foods and health products, cosmetics and medicines.

The invention discloses a lactobacillus reuteri 22 and application thereof. The lactobacillus reuteri 22 disclosed by the invention is separated from healthy chicken flocks, is classified and named aslactobacillus reuteri, and has a preservation number of CGMCC No.17932. By detecting the performance of probiotics, the invention finds that the lactobacillus reuteri 22 has good acid resistance, cholate resistance and adhesion performance, and can inhibit growth of pathogenic bacteria. Through Animal assays, in a healthy physiological state, the lactobacillus reuteri 22 does not influence the level of inflammatory factors, but can increase the number of goblet cells and promote the increase of expression levels of related genes such as compact protein, defensin and lysozyme, can increase thelength of intestinal villi and the depth of crypt, and has the potential of maintaining intestinal mucosa barriers. Therefore, the strain is expected to be developed into probiotics for maintaining achicken intestinal mucosa barrier and guaranteeing green and healthy chicken breeding.

The invention relates to a preparation method for a health drink containing grosvener siraitia extract and active bifidobacterium longum, and relates to a drink prepared by the preparation method, belonging to the technical field of food drink. The preparation comprises the following steps: sterilizing a fermentation culture solution which takes grosvener siraitia extract, xylooligosaccharide, soybean polysaccharide, soy peptone, skim milk, calcium lactate 3, composite barbary wolfberry fruit concentrated juice, and the like as the main raw materials according to a special condition; inoculating a plant lactobacillus plantarum bacterium solution and a bifidobacterium longum bacterium solution in sequence, keeping a certain tank pressure in an anaerobic fermentation tank to obtain fermentation milk base stock, filling or mixing and filling, thereby obtaining the health drink containing grosvener siraitia extract and active bifidobacterium longum. The health drink containing grosvener siraitia extract and active bifidobacterium longum has high viable organism content, short fermentation time and a high viable organism survival rate, and has a remarkable prebiotics effect, wherein the fermentation, the acid resistance and the gastric acid resistance of the bifidobacterium longum are good.

The invention discloses a multi-joint bacterium lacticum composition. The multi-joint bacterium lacticum composition is an advantage bacterial strain selected by a separating screen in the vagina of ahealthy female in China and at least comprises three active components of Lactobacillus johnsonii (Lactobacillus johnsonii), Lactobacillusgasseri (Lactobacillusgasseri), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus crispatus ( Lactobacillus crispatus ) and Lactobacillus jensenii (Lactobacillus jensenii ).

The invention provides lactobacillus helveticus and application thereof in the bee breeding process. The lactobacillus helveticus strain is preserved in the China General Microbiological Culture Collection Center on July 2, 2018, has a preservation number of CGMCC No.16042, and is classified and namely as lactobacillus helveticus KM7. After the lactobacillus helveticus is cultured in an MRS agar culture medium, the lactobacillus helveticus, and has a round, milky white, neat-edged, smooth-faced, raised and non-transparent colonial form. The lactobacillus helveticus KM7 is spontaneously separated from intestines of Eastern worker bees which are cultured in the bee farm of the bee science department of animal science and technology college of the Yunnan Agricultural University in Kunming, Yunnan Province and freely digest flower honey in spring, has relatively good bacteriostatic activity, and is sensitive to multiple antibiotics. The lactobacillus helveticus KM7 is added into syrup feeding bees in an amount of 108CFU / mL, so that the floral microecological structure in the intestines of bees can be improved, the intestinal immunity of bees can be enhanced, and the survival rate of bees can be improved.

The invention provides a composite probiotic-fermented alfalfa sprout powder preparation and a preparation method and application thereof. Tactobacillus plantarum SCI-01, lactobacillus plantarum P-8, bifidobacterium animalis lactis V9 and lactobacillus casei Zhang are taken as fermentation bacteria, and the preservation numbers of the tactobacillus plantarum SCI-01, the lactobacillus plantarum P-8, the bifidobacterium animalis lactis V9 and the lactobacillus casei Zhang are CGMCC No.11930, CGMCC No.6312, CGMCC No.5470 and CGMCC No.5469; a fermentation culture medium is prepared from, by weight, 970-1,000 parts of alfalfa sprout powder, 8-12 parts of sucrose and 8-12 parts of soybean protein powder, and the material-water ratio is 1:1; the initial pH value is 6.0, the total inoculation quantity is 1*10<7> CFU / g, fermentation is performed at 37 DEG C, and fermentation stops when the pH value is 5.0; a fermentation product is subjected to vacuum freeze drying to obtain the probiotic alfalfa raw powder preparation, the live bacterium number of probiotics reaches 1.0*10<10> CFU / g or above, and the probiotic alfalfa raw powder preparation can be prepared into tablets, powder or capsules after being directly mixed with xylitol.

The present invention provides a composition for regulating human body intestinal micro-ecology. The composition comprises stachyose and oligomannose as effective active ingredients. The composition provided by the invention can effectively regulate intestinal flora functions.

The invention relates to the technical field of microorganisms, in particular to probiotic lactobacillus plantarum and application thereof in preparation of low-salt fermented meat food. The invention provides a strain of lactobacillus plantarum CMRC 19L, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.11347. The lactobacillus plantarum CMRC 19L is high in growth speed, capable of inhibiting growth of pathogenic bacteria, excellent in meat fermentation performance, resistant to the gastrointestinal tract environment, high in adhesiveness to intestinal mucus protein and intestinal epithelioid cells and good in probiotic performance. As a leavening agent, the strain can ensure the edible safety and flavor quality of the low-salt fermented meat food, and significantly improve the nutritional value of the fermented meat food.

The invention provides lactobacillus plantarum and application thereof. The lactobacillus plantarum is named as LPZN19 and preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO: 22770. The lactobacillus plantarum is used as a food leavening agent to prepare fermented food, such as Sichuan sausages, biogenic amines in the Sichuan sausages can be degraded, the degradation rate of total biogenic amines after fermentation (30 days) is completed is 28.79%, and the degradation rates of histamine, tyramine, cadaverine, putrescine, tryptamine, phenylethylamine and spermidine correspondingly reach 41.95%, 24.07%, 54.19%, 10.76%, 24.90%, 8.07% and 5.3%. The lactobacillus plantarum is screened from the fermented sausages and has excellent probiotic characteristics, so that the lactobacillus plantarum is particularly suitable for production of fermented meat products such as preserved meat and hams, and important practical significance is achieved in research and development of functional lactic acid bacteria leavening agents, establishment of lactic acid bacteria strain resource libraries and solving of food safety problems to a certain extent.

The invention discloses a genetic engineering subunit oral vaccine strain for preventing porcine epidemic diarrhea as well as a construction method and an application thereof. Autochthonous lactic acid bacteria strains which are separated from intestinal tract of pigs are used as transferring active carriers of vaccine antigens, PEDV S protein neutralizing antigen core epitope domain COE is selected as an immunogen, and at the same time transport speed of the vaccine antigen is effectively improved, immune response time of bodies is shortened, an antigen transporting cell M cell targeting peptide Col and dendritic cell targeting peptide DCpep for antigen processing and presentation is introduced, a constitutive expression carrier is used for constructing a recombined lactic acid bacteria preparation for expressing the PEDV S protein neutralizing antigen core epitope domain COE and a M cell targeting peptide Col and a dendritic cell targeting peptide DCpep, so that the vaccine antigen secretes along with breeding of genetic engineering bacterial strains, and disadvantages of induction type recombined lactic acid bacteria are avoided. An effective technical means is provided for developing the genetic engineering subunit oral vaccine strain for preventing porcine epidemic diarrhea is provided.

The invention relates to field of feeding probiotics, and particularly relates to a lactobacillus strain with high adhesive power, and an application thereof. The lactobacillus has a good adhesion function for suppressing pathogenic bacteria. The lactobacillus strain with high adhesive power is preserved in China General Microbiological Culture Collection Center (Institute of Microbiology Chinese Academy of Sciences, No.3 Yard No.1, West Beichen Road, Chaoyang District, Beijing 100101, China) on December 9th, 2013, with a preservation number of CGMCC No. 8542. A strain number is ZJ617; and a classification name is lactobacillus reuteri. The lactobacillus strain (ZJ617) with high adhesive power is a strain providing efficiently high adhesion power mode for studying an adhesion mechanism of probiotics or lactobacillus and has important practical significance for developing novel microecological preparations, applying microorganism feed additives, enhancing yoghourt quality, and improving microorganism steady state of human and animals.

The invention relates to a lactobacillus plantarum RS-09 with high adhesion and applications thereof, and belongs to the field of microorganisms. The strain is separated from apple juice, and is preserved in in the China General Microbiological Culture Collection Center with a preservation number of CGMCC NO.17118; has stronger acid resistance and choline salt resistance, can be stored for a longtime, has higher adhesion capacity to human colon cancer cells HT-29, and can be stably planted in intestinal tracts, wherein the planting rate of the cecum is highest; can obviously reduce the content of TNF-alpha in serum, increase the content of IL-10 and IL-4 in the serum, and has the function of immunoregulation; and has strong bacteriostatic effect on salmonella enteritis, has prevention andtreatment effects on enteritis diseases caused by flora disorder, can be applied to the field of functional foods, not only has practical production value, but also has very important significance onbody health.

The invention discloses a strain of German-style lactobacillus which is separated from traditional fermented yak yoghurt in Tibetan Plateau and has oxidation resistance and bacteriocin producing capability. The preservation number is CGMCC No.11247, the activity of GSH-Px and T-SOD in mouse bodies can be remarkably improved, the content of MDA in serum of mice is decreased, and the German-style lactobacillus has the immunoregulation function and can be applied to preparation of fermented milk products and health-care food and medicine.

The invention provides lactobacillus paracasei SLP16 for producing polysaccharides, application of the lactobacillus paracasei SLP16 and a feed additive prepared from the lactobacillus paracasei SLP16, and relates to the technical field of feed additives. The lactobacillus paracasei SLP16 disclosed by the invention is Lactobacillus paracasei SLP16; the Latin name is Lactobacillus paracasei, the name of the preservation unit is Guangdong Microbial Culture Collection Center; the address is Floor 5, Building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou city; the preservation date is July 5, 2021; and the preservation number is GDMCCNo: 61769. The SLP16 disclosed by the invention is still stable after passage, can inhibit escherichia coli and staphylococcus aureus, has good cholate-resistant and acid-resistant effects, has cohesiveness, hydrophobicity and oxidation resistance, and also can generate exopolysaccharides.

The invention belongs to the technical field of biology, and provides lactobacillus reuteri and application thereof in a bee breeding process. The lactobacillus reuteri is preserved in the China General Microbiological Culture Collection Center on July 2, 2018, has a preservation number of CGMCC No.16043, and is classified and named as lactobacillus reuteri LP4. The lactobacillus reuteri is cultured in an MRS agar culture-medium, has round, milky white, neat-edged, smooth-faced, raised and non-transparent colonial form. The lactobacillus reuteri LP4 is spontaneously separated from intestines of Eastern worker bees which are bred by beekeepers in the Luoping County of Yunnan Province and freely digest rape flower honey in spring, has relatively good bacteriostatic activity, and is sensitiveto multiple antibiotics. The lactobacillus reuteri LP4 is added into syrup feeding bees in an amount of 108CFU / mL, so that the floral microecological structure in the intestines of bees can be improved, the intestinal immunity of bees can be enhanced, and the survival rate of bees can be improved.

The invention discloses a composition for treating colitis, medicine and a preparing method thereof. According to the composition for treating colitis, probiotics and bovine coloctrum growth factors are used in cooperation. Balb / c mice are used as test objects, a colitis model is set up, and the colitis resisting effect of the medicine is studied from the aspects of the apparent state, the body weight change, the colon length, the pathology state and the like of the test mice. The test results show that the effect of the medicine is better than that of medicine singly using the probiotics or the bovine coloctrum growth factors; the the probiotics and the bovine coloctrum growth factors have a cooperative synergism effect, the medicine has a good colitis resistant effect, colitis will not relapse within a short term, and intestinal mucosa injuries caused by colitis can be well repaired.

The invention discloses a fermented fruit and vegetable juice and a making method thereof. The raw materials comprise vegetables and fruits, the vegetables include white radish, carmine radish, red radish, celery, cabbage, Chinese cabbage, cucumber and carrot, the fruits comprise apple, pear, orange, pineapple, small persimmon, kiwi and strawberry, and the sum of the added amount of vegetables andfruits in the juice is 20-50% of the total weight of the product, and the ratio of vegetables to fruits is 1 :2-2:1, and the fermentation is carried out using a probiotic which is a mixture of one orboth of Lactobacillus plantarum and Lactobacillus paracasei. The fermented fruit and vegetable juice of the invention is obtained by adding fruits and vegetables to be fermented together, and the fermented fruit and vegetable juice has moderate acidity, good flavor and nutrient composition of fruits and vegetables, and is a nutritious and safe fruit and vegetable juice product. The fermented fruit and vegetable juice not only improves the sensory quality of fermented fruit and vegetable juice, but also increases the nutritional value, prolongs the shelf life, and meets the modern consumer's healthy consumption concept.

The invention discloses a double-protein fermented milk beverage rich in active Lactobacillus plantarum and a preparation method thereof, and belongs to the technical field of fermentation engineering. The preparation method includes the following steps: inoculating Lactobacillus plantarum into double-protein base material components with the addition of proliferating factors and inorganic salts for fermentation to obtain fermented milk, and then adding stabilizers, sweeteners and water into the fermented milk for blending to obtain the fermented milk beverage; the preparation method can successfully realize the proliferation and fermentation of the Lactobacillus plantarum in the double-protein base material, and can greatly shorten the fermentation time of the Lactobacillus plantarum in the double-protein base material; the fermented milk beverage obtained by the preparation method is high in probiotic content, and the viable count can reach 5.0 x 10 8(superscript) cfu / mL or above; regarding the fermented milk beverage, the product system is stable, the protein content meets the requirements of national standards, sourness and sweetness are palatable, and the cost can be controlled; and the fermented milk beverage obtained by the preparation method is performed with low temperature storage at 0-10 DEG C for 21 days, and the viable count can be stabilized at 3.0 x 10 7(superscript) cfu / mL or above.