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Lactobacillus strain with high adhesive power, and application thereof

A lactobacillus, high-adhesion technology, applied in applications, bacteria, dairy products, etc., can solve the problems of few model strains, lack of adhesion protection and mechanism, etc., to enhance the quality of yogurt, improve human and animal microbial homeostasis, The effect of high social benefit

Inactive Publication Date: 2015-01-21
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, domestic and foreign studies have shown that the adhesion of probiotics and lactobacillus is strain-specific, and there are few model strains with high adhesion performance, and there is still a lack of protection and mechanism research data related to adhesion

Method used

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  • Lactobacillus strain with high adhesive power, and application thereof
  • Lactobacillus strain with high adhesive power, and application thereof
  • Lactobacillus strain with high adhesive power, and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The present invention obtains mucus and chyme from the intestinal tract of weaned piglets at the age of 28 days, and then applies anaerobic culture on the plate, purifies and cultures a single clone to obtain pure colonies, and obtains 85 strains of Lactobacillus (partial strains such as figure 1), including 36 strains of jejunal chyme, 3 strains of ileal chyme, 18 strains of jejunal mucus and 28 strains of ileal mucus (such as figure 2 ).

[0037] The obtained pure bacteria were stained with Methylene blue, and the lactic acid bacteria were initially screened out according to the morphology under the microscope, and then 23 kinds of sugar fermentation biochemical tubes were used to confirm the type of lactobacillus. The biochemical tubes were lactose, maltose, mannitol, sucrose, arabinose, and xylose , trehalose, raffinose, fructose, rhamnose, galactose, cellobiose, melebiose, melezitose, mannose, sorbitol, arabitol, salicin, escin, gluconate, Arginine hydrolysis, D-...

Embodiment 2

[0080] Embodiment 2 contains the dairy product of bacterial strain of the present invention

[0081] Raw material formula (according to 1 ton):

[0082]

[0083] Different probiotic strains have more specific and different characteristics and functions, and the beneficial effects on human health after taking are also different. On the basis of the combination of three specific probiotics, Lactobacillus acidophilus, Bifidobacterium lactis and Bifidobacterium longum, the present invention adds Lactobacillus ZJ617 with high adhesion force to make yogurt, and the yogurt product of the present invention is processed The results of relevant clinical medical testing and evaluation show that the yogurt containing the ZJ617 probiotic combination of the present invention has a comprehensive and superior effect of promoting human intestinal health, which is higher than that reported in the prior art. Lactobacillus acidum, Bifidobacterium lactis and Bifidobacterium longum are the sum ...

Embodiment 3

[0086] A probiotic microbial feed additive includes the high-adhesion Lactobacillus ZJ617, the feed additive is composed of Lactobacillus ZJ617 and a carrier, and the mass ratio of Lactobacillus ZJ617 to the carrier is generally controlled at 2-3:15-30 The carrier is any one or a mixture of two or more of microcrystalline kaolinite, shell powder, sweet potato starch and corn starch.

[0087] Specifically, the present embodiment provides a formula in which Lactobacillus ZJ617 is mixed with a carrier in a mass ratio of 3:16, the carrier is a mixture of microcrystalline kaolinite, shell powder, sweet potato starch and corn starch, and the mass ratio is 1:1 :1:2. The lactobacillus ZJ617 and the carrier are stirred at 300-350 rpm for 10-20 minutes and mixed evenly to prepare the probiotic microbial feed additive.

[0088] The probiotic microbial feed additive provided by the invention is used in aquatic feed, and its added amount is 0.5-2% of the total feed weight; used in full-pr...

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Abstract

The invention relates to field of feeding probiotics, and particularly relates to a lactobacillus strain with high adhesive power, and an application thereof. The lactobacillus has a good adhesion function for suppressing pathogenic bacteria. The lactobacillus strain with high adhesive power is preserved in China General Microbiological Culture Collection Center (Institute of Microbiology Chinese Academy of Sciences, No.3 Yard No.1, West Beichen Road, Chaoyang District, Beijing 100101, China) on December 9th, 2013, with a preservation number of CGMCC No. 8542. A strain number is ZJ617; and a classification name is lactobacillus reuteri. The lactobacillus strain (ZJ617) with high adhesive power is a strain providing efficiently high adhesion power mode for studying an adhesion mechanism of probiotics or lactobacillus and has important practical significance for developing novel microecological preparations, applying microorganism feed additives, enhancing yoghourt quality, and improving microorganism steady state of human and animals.

Description

technical field [0001] The invention relates to the field of probiotics for feeding, in particular to a strain of lactobacillus with high adhesion and application thereof. Background technique [0002] Human beings, animals and plants that exist in nature have a large number of microorganisms participating in activities no matter on the surface or in the body. Microorganisms and hosts have their own physiological and biochemical systems, but there is a relationship of mutual promotion and mutual restriction between the two. The normal physiological and biochemical parameters and pathological parameters of the host are the result of the interaction between the two. The intestinal tract of humans and animals is a microenvironment with biodiversity, in which there are hundreds of microbial flora, and the interaction between different microbial flora constitutes the intestinal microbial homeostasis. The composition of intestinal flora in normal humans and animals is relatively...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16A23C9/127C12R1/225
CPCA23C9/127C12N1/205C12R2001/225
Inventor 汪海峰刘建新高侃章文明张宏福王翀
Owner ZHEJIANG FORESTRY UNIVERSITY
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