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Lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and microbial strains, applied in the direction of Lactobacillus, application, bacteria, etc., can solve problems such as human health hazards, affecting product flavor, human heart and central nervous system damage, etc.

Active Publication Date: 2021-09-10
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive biogenic amines will not only cause harm to human health, but also cause the expansion of blood vessels, arteries and microvessels, such as high blood pressure, headache, abdominal cramps, diarrhea and vomiting, and also cause damage to the human heart and central nervous system. It also affects the flavor of the product

Method used

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  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Screening and Identification of Biogenic Amine Degrading Bacteria

[0041] 1.1 Isolation and purification of biogenic lactic acid bacteria

[0042] Take 25g of meat sample and mix it homogeneously in 225mL sterile normal saline, take 1mL and add it to MRS broth medium for enrichment culture, and then use 0.8% normal saline for gradient dilution (from 10 -1 to 10 -7 ), take 200 μL of the diluted solution and spread it on the MRS solid medium containing 0.8% calcium carbonate and cultivate it for 24 hours. 3 times. Such as figure 1 As shown, the amine-producing bacteria are purple within 5 minutes, and the non-amine-producing bacteria are yellow, and the color is developed twice in parallel. The strains showing yellow color twice were separated and purified on MRS medium for 3 times, and then stored on a slant plane at 4°C for future use.

[0043] 1.2 Initial screening of strains

[0044] Lactic acid bacteria that can be used as a starter for fermented meat products...

Embodiment 2

[0126] The application of No. 19 Lactobacillus plantarum of embodiment 1 in making Sichuan flavor sausage, concrete process is as follows:

[0127] 2.1 Processing technology formula

[0128] Beef 8kg, pig fat 2kg, salt 0.25kg, white sugar 0.1kg, chili powder 0.1kg, pepper powder 0.04kg, thirteen spices 0.005kg, monosodium glutamate 0.015kg, glucose 0.01kg, garlic powder 0.005kg, black pepper 0.002kg 、NaNO 2 0.00075kg, liquor 0.1kg.

[0129] 2.2 Key points of processing operation

[0130] Raw meat: remove beef skin, bone and connective tissue, and wash with water;

[0131] Grinding: Pre-cool the raw meat at 4°C, put the beef and pig fat together into a sausage slicer;

[0132] Pickling: add salt pickling preparation, pickle at 4°C for 4 hours;

[0133] Stirring: adding spices and accessories to the marinated meat;

[0134] Inoculation starter: the inoculum concentration is 10 7 CFU / g, the starter is made by mixing Lactobacillus plantarum, Pediococcus pentosaceus and Stap...

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Abstract

The invention provides lactobacillus plantarum and application thereof. The lactobacillus plantarum is named as LPZN19 and preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO: 22770. The lactobacillus plantarum is used as a food leavening agent to prepare fermented food, such as Sichuan sausages, biogenic amines in the Sichuan sausages can be degraded, the degradation rate of total biogenic amines after fermentation (30 days) is completed is 28.79%, and the degradation rates of histamine, tyramine, cadaverine, putrescine, tryptamine, phenylethylamine and spermidine correspondingly reach 41.95%, 24.07%, 54.19%, 10.76%, 24.90%, 8.07% and 5.3%. The lactobacillus plantarum is screened from the fermented sausages and has excellent probiotic characteristics, so that the lactobacillus plantarum is particularly suitable for production of fermented meat products such as preserved meat and hams, and important practical significance is achieved in research and development of functional lactic acid bacteria leavening agents, establishment of lactic acid bacteria strain resource libraries and solving of food safety problems to a certain extent.

Description

technical field [0001] The invention belongs to the technical field of microbial fermented food, and in particular relates to a strain of Lactobacillus plantarum and its application. Background technique [0002] Sichuan-flavored sausage is one of Sichuan's traditional fermented meat products. It usually uses chili, pepper, and pepper ("three peppers") as auxiliary materials. It generally needs to be fermented and smoked. "Intestine" is deeply loved by consumers because of its rich aroma and delicious taste. [0003] The fermentation process of Sichuan-style sausage mainly depends on the environment and the action of microorganisms in raw meat, and these microorganisms may produce biogenic amines (Biogenic amines, BAs). BAs are biologically active small molecular weight nitrogen-containing organic compounds formed by the decarboxylation of amino acids or the amination of aldehydes and ketones. In fermented sausages, the common BAs mainly include histamine, tyramine, cadave...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L5/20A23L13/60A23L13/40C12R1/25C12R1/01C12R1/44
CPCC12N1/20A23L5/28A23L13/65A23L13/45A23V2400/427A23V2400/169Y02A40/90
Inventor 杨勇王艺伦裴慧洁邓霖兰沁洁刘雨萱黄晓红敖晓琳刘书亮
Owner SICHUAN AGRI UNIV
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