Lactobacillus plantarum and application thereof
A technology of Lactobacillus plantarum and microbial strains, applied in the direction of Lactobacillus, application, bacteria, etc., can solve problems such as human health hazards, affecting product flavor, human heart and central nervous system damage, etc.
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Embodiment 1
[0040] Screening and Identification of Biogenic Amine Degrading Bacteria
[0041] 1.1 Isolation and purification of biogenic lactic acid bacteria
[0042] Take 25g of meat sample and mix it homogeneously in 225mL sterile normal saline, take 1mL and add it to MRS broth medium for enrichment culture, and then use 0.8% normal saline for gradient dilution (from 10 -1 to 10 -7 ), take 200 μL of the diluted solution and spread it on the MRS solid medium containing 0.8% calcium carbonate and cultivate it for 24 hours. 3 times. Such as figure 1 As shown, the amine-producing bacteria are purple within 5 minutes, and the non-amine-producing bacteria are yellow, and the color is developed twice in parallel. The strains showing yellow color twice were separated and purified on MRS medium for 3 times, and then stored on a slant plane at 4°C for future use.
[0043] 1.2 Initial screening of strains
[0044] Lactic acid bacteria that can be used as a starter for fermented meat products...
Embodiment 2
[0126] The application of No. 19 Lactobacillus plantarum of embodiment 1 in making Sichuan flavor sausage, concrete process is as follows:
[0127] 2.1 Processing technology formula
[0128] Beef 8kg, pig fat 2kg, salt 0.25kg, white sugar 0.1kg, chili powder 0.1kg, pepper powder 0.04kg, thirteen spices 0.005kg, monosodium glutamate 0.015kg, glucose 0.01kg, garlic powder 0.005kg, black pepper 0.002kg 、NaNO 2 0.00075kg, liquor 0.1kg.
[0129] 2.2 Key points of processing operation
[0130] Raw meat: remove beef skin, bone and connective tissue, and wash with water;
[0131] Grinding: Pre-cool the raw meat at 4°C, put the beef and pig fat together into a sausage slicer;
[0132] Pickling: add salt pickling preparation, pickle at 4°C for 4 hours;
[0133] Stirring: adding spices and accessories to the marinated meat;
[0134] Inoculation starter: the inoculum concentration is 10 7 CFU / g, the starter is made by mixing Lactobacillus plantarum, Pediococcus pentosaceus and Stap...
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