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Applications of polyetherimide in preparing medicines for preventing influenza virus infection

A polyetherimide, influenza prevention technology, applied in the field of medicine, can solve the problems of unseen diseases, weak induction effect, etc.

Inactive Publication Date: 2017-07-07
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, the internationally renowned magazine "Nature Biotechnology" reported that PEI has a very strong adjuvant effect, and the cytokines it induces are biased towards Th2 type cytokines, such as IL-4, IL-5 and IL-13, etc., but for IL-17, The induction effect of IFN-γ and TNF-α is relatively weak (reference Polyethyleneimine is a potent mucosal adjuvant for viral glycoproteinantigens. Nat. Biotechnol. 2012Sep; 30(9):883-8.)
So far, there are no relevant reports on the prevention of diseases caused by influenza virus infection with PEI at home and abroad.

Method used

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  • Applications of polyetherimide in preparing medicines for preventing influenza virus infection
  • Applications of polyetherimide in preparing medicines for preventing influenza virus infection
  • Applications of polyetherimide in preparing medicines for preventing influenza virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Evaluation of anti-influenza virus activity in PEI animals

[0024] 1. Experimental materials

[0025] Balb / C female mice (about 10 weeks), PEI (linear 25kD, sigma), polyIC (invivogen), CTB (sigma), H1N1 (A / PR / 8 / 34), PEI, polyIC and CTB are all dissolved in PBS middle.

[0026] 2. Experimental methods and results

[0027] After Balb / C female mice were anesthetized with pentobarbital sodium, PEI (20 μg), polyIC (20 μg), CTB (2.5 μg) and PBS were administered nasally, with a volume of 50 μl, administered twice with an interval of 24 hours. 5LD was administered to mice 24 hours after the second dose 50A dose of H1N1 influenza virus was used to infect mice, and the weight loss and survival rate of mice after challenge were recorded for 14 consecutive days. In addition, the lungs of 4 mice in each group were taken on the 2nd and 4th day for the detection of lung virus titer and the lungs of 4 mice in each group were taken on the 0th day, 2nd day and 4th day F...

Embodiment 2

[0028] Example 2: Research on the Anti-Influenza Virus Mechanism of PEI in vivo

[0029] 1. Experimental materials

[0030] RNA extraction kit (Kangwei reagent), reverse transcription kit (Kangwei reagent), qPCR kit (vazyme)

[0031] 2. Experimental methods and results

[0032] On day 0, day 2 and day 4, the lungs of 4 mice in each group were used for the detection of cytokines. The method of cytokine detection is qRT-PCR (reference Intranasal treatment with poly(I·C) protects aged mice from lethal respiratory virus infections. J Virol. 2012 Nov; 86(21): 11416-24.). Previous reports showed interferon (reference Protection from pulmonary tissue damage associated with infection of cynomolgus macaques by highly pathogenic avian influenza virus (H5N1) by low dose natural human IFN-αadministered to thebuccal mucosa. Antiviral Res. 2014Oct; 110:175-80), GM-CSF (reference Delivery ofGM-CSF to Protect against Influenza Pneumonia.PLoS One.2015Apr29; 10(4):e0124593) and IFITM3 (refer...

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Abstract

The invention belongs to the technical field of medicines, and in particular relates to applications of polyetherimide in preparing medicines for preventing influenza virus infection. The influenza virus infection animal model and the animal protection experiment developed on the influenza virus infection animal model prove that after nasal-passage administration of polyetherimide in advance, the mice can resist the infection of H1N1 with the lethal dose, which proves that polyetherimide has the function of preventing influenza virus infection; the research result of the action mechanism shows that polyetherimide has the obvious advantages compared with other immunity activators, can induce antiviral factors, and has the small induction effect for IL-17 which can aggravate the conditions of influenza virus. Polyetherimide can be further developed to be prepared into the medicines for preventing influenza virus infection, and has the wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically relates to the application of polyetherimide in the preparation of medicines for preventing influenza virus infection. Background technique [0002] Influenza virus, referred to as influenza virus for short, belongs to the family Orthomyxoviridae and belongs to the genus Influenza virus. Human influenza viruses can be divided into three types A, B, and C according to the antigenicity of their nucleoproteins and matrix proteins, also known as types A, B, and C. Among them, type A (type A) influenza can be further divided into HXNX subtypes, such as H5N1 and H7N9, according to the antigenicity of hemagglutinin HA and neuraminidase NA on the surface of the virus. [0003] Influenza caused by influenza virus is a respiratory infectious disease that seriously threatens human health. Every year, seasonal influenza in the world will cause 3 to 5 million people to have severe symp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/787A61P31/16
Inventor 姜世勃陆路贺彪
Owner FUDAN UNIV
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